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INH-B elisa kit :: Mouse inhibin B ELISA Kit

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Catalog # MBS700883
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Typical Testing Data/Standard Curve (for reference only)
Product Name

inhibin B (INH-B), ELISA Kit

Popular Item
Full Product Name

Mouse inhibin B, INH-B ELISA Kit

Product Synonym Names
Mouse inhibin B; INH-B ELISA Kit; EDF; FRP; FSH-releasing protein; Inhibin; beta-1; OTTHUMP00000207260; erythroid differentiation factor; follicle-stimulating hormone-releasing protein; inhibin beta A; inhibin; beta A (activin A; activin; inhibin B
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS700883 Testing Data
3D Structure
ModBase 3D Structure for Q04998
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse INH-B. No significant cross-reactivity or interference between Mouse INH-B and analogues was observed.
Detection Range
0.8 pg/ml-200 pg/ml
Sensitivity
0.2 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of INH-B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for INH-Bpurchase
MBS700883 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the inhibin B (INH-B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing INH-B. The ELISA analytical biochemical technique of the MBS700883 kit is based on INH-B antibody-INH-B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect INH-B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, INH-B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
INH-B elisa kit
Introduction: Inhibin is a peptide that is an inhibitor of FSH synthesis and secretion, and participates in the regulation of the menstrual cycle. Inhibin contains an alpha and beta subunit linked by disulfidebonds. Two forms of inhibin differ in their beta subunits (A or B), while their alpha subunits are identical. Inhibin belongs to the transforming growth factor-beta (TGF-beta) superfamily. In women, FSH stimulates the secretion of inhibin from the granulosa cells of the ovarian follicles in ovary. In turn, inhibin suppresses FSH.Inhibin secretion is diminished by GnRH, and enhanced by insulin-like growth factor-1 (IGF-1). Inhibins have been defined based on their activity of suppressing pituitary gonadotropin secretion. Thus, the serum concentrations of Inhibin B and FSH are inversely correlated, and at low serum levels of Inhibin B, FSH concentration goes up. Inhibin B serum concentration increases gradually in the follicular phase to a broad peak at 7 days prior to the LH surge, and may constitute the limiting factor for the duration of the inter-cycle FSH rise. A decrease and a second peak follow this peak one-day past the LH surge. Most of the serum Inhibin B concentration originates from large follicles, since such follicles secrete 10 fold higher concentrations than do the small follicles. Inhibin B concentrations drop to a very low concentration at the luteal phase, and is undetectable during pregnancy.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated INH-B and antibody preparation specific for INH-B, and incubated. Then substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of INH-B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of INH-B elisa kit
INH-B elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS700883. Click to request current manual
NCBI/Uniprot data below describe general gene information for INH-B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
47,392 Da
NCBI Official Full Name
inhibin beta A chain
NCBI Official Synonym Full Names
inhibin beta-A
NCBI Official Symbol
Inhba  [Similar Products]
NCBI Protein Information
inhibin beta A chain; activin beta-A chain
UniProt Protein Name
Inhibin beta A chain
UniProt Synonym Protein Names
Activin beta-A chain
Protein Family
UniProt Gene Name
Inhba  [Similar Products]
UniProt Entry Name
INHBA_MOUSE
UniProt Comments for INH-B
Function: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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