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INHBA elisa kit :: Human Activin A ELISA Kit

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Catalog # MBS175781
Unit / Price
  96-Strip-Wells  /  $380 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Activin A (INHBA), ELISA Kit

Popular Item
Also Known As

Human Activin A PicoKine ELISA Kit

Product Synonym Names
Inhibin beta A chain; inhibin, beta A; Activin beta A chain; erythroid differentiation factor; erythroid differentiation protein; follicle stimulating hormone releasing protein; FSH releasing protein; Inhibin; inhibin beta A chain
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
426
3D Structure
ModBase 3D Structure for A4D1W7
Species Reactivity
Specificity
Natural and recombinant human Activin A
Samples
Cell culture supernates, serum, plasma (heparin, EDTA) and saliva
Assay Type
Quantitative Sandwich
Detection Range
15.6pg/ml- 1000pg/ml
Sensitivity
<12pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of INHBA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forINHBApurchase
MBS175781 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Activin A (INHBA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing INHBA. The ELISA analytical biochemical technique of the MBS175781 kit is based on INHBA antibody-INHBA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect INHBA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, INHBA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
INHBA elisa kit
Principle of the Assay: The Human Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Activin A has been precoated onto 96-well plates. Standards(Expression system for standard: CHO ; Immunogen sequence: G311-S426) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Leptin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. Acvidin-Biotin-Peroxidase Compiex was added and unbound conugates were washed away with PBS or TBS buffer HRP substrate TMB was used to visualize HRP enzymatic tion. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Activin A amount of sample captured in plate.

Background: Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of Ire transforming growth factor-beta superfamily, is expressed loca!ly by the mesenchymal component of the hemopoietlc microenvironment. its expression is regulated on the mRNA level by different cytoKines. and the biologilt activity of the protein is... controlled by several inhibitory molecules.' Inhibins and activins are members of ine transforming growth factor beta superfamily and are known to modulate the growth and differentiation of severe I cell types 2 Inhibins and activins inhibit and activate. respectively. the secretion of follitropin by the pituitary glanj. Inhibins/activins are Involved in regulating a number of diverse functions such as hypothalamic and pituitary horlnone secretion, gonadal hormone secretion, germ celi development and maturation. erythroid differentiation. insulin secretion. nerve cell survival embryonic axial development or bone growth depending on their subunit composition. Inhibins appear to oppose the functions of activins.

Typical Testing Data/Standard Curve (for reference only) of INHBA elisa kit
Human Activin A PicoKine ELISA Kit standard curve
INHBA elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS175781. Click to request current manual
NCBI/Uniprot data below describe general gene information for INHBA. It may not necessarily be applicable to this product.
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
47,442 Da
NCBI Official Synonym Full Names
inhibin, beta A
NCBI Official Symbol
INHBA  [Similar Products]
NCBI Official Synonym Symbols
EDF; FRP
  [Similar Products]
NCBI Protein Information
inhibin beta A chain; Inhibin, beta-1; activin beta-A chain; FSH-releasing protein; erythroid differentiation factor; erythroid differentiation protein; follicle-stimulating hormone-releasing protein; inhibin, beta A (activin A, activin AB alpha polypeptide)
UniProt Protein Name
Inhibin, beta A (Activin A, activin AB alpha polypeptide)
UniProt Synonym Protein Names
Inhibin, beta A (Activin A, activin AB alpha polypeptide)
Protein Family
UniProt Gene Name
INHBA  [Similar Products]
UniProt Entry Name
A4D1W7_HUMAN
NCBI Summary for INHBA
The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome. [provided by RefSeq, Jul 2008]
UniProt Comments for INHBA
INHBA: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p15-p13

Cellular Component: extracellular region

Molecular Function: identical protein binding; protein binding; growth factor activity; protein heterodimerization activity; peptide hormone binding; hormone activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; activin receptor signaling pathway; mesodermal cell differentiation; defense response; palate development; negative regulation of cell cycle; negative regulation of interferon-gamma biosynthetic process; odontogenesis; negative regulation of cell proliferation; cell surface receptor linked signal transduction; hair follicle development; cell-cell signaling; ovarian follicle development; erythrocyte differentiation; cell differentiation; cell cycle arrest; negative regulation of B cell differentiation; hemoglobin biosynthetic process; response to drug; nervous system development; regulation of follicle-stimulating hormone secretion; positive regulation of erythrocyte differentiation; male gonad development; progesterone secretion; negative regulation of follicle-stimulating hormone secretion; regulation of transcription from RNA polymerase II promoter; negative regulation of macrophage differentiation; regulation of MAPKKK cascade; negative regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; hemopoietic progenitor cell differentiation; negative regulation of cell growth; positive regulation of follicle-stimulating hormone secretion; cell development; growth; G1/S transition of mitotic cell cycle
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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