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INHbetaC elisa kit :: Human Inhibin beta C ELISA Kit

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Catalog # MBS729519
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Inhibin beta C (INHbetaC), ELISA Kit

Popular Item
Also Known As

Human Inhibin beta C ELISA Kit

Product Synonym Names
Human Inhibin b C ELISA Kit
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
601233
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of INHbetaC. No significant cross-reactivity or interference between INHbetaC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between INHbetaC and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
50-1000pg/mL
Sensitivity
1.0pg/mL
Intended Uses
This INHbetaC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human INHbetaC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of INHbetaC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forINHbetaCpurchase
MBS729519 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Inhibin beta C (INHbetaC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing INHbetaC. The ELISA analytical biochemical technique of the MBS729519 kit is based on INHbetaC antibody-INHbetaC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect INHbetaC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, INHbetaC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
INHbetaC elisa kit
Principle of the assay: INHbetaC ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for INHbetaC. Standards or samples are then added to the microtiter plate wells and INHbetaC if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of INHbetaC present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for INHbetaC are added to each well to "sandwich" the INHbetaC immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain INHbetaC and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INHbetaC concentration in each sample is interpolated from this standard curve.
Product Categories/Family for INHbetaC elisa kit

Typical Testing Data/Standard Curve (for reference only) of INHbetaC elisa kit
INHbetaC elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS729519. Click to request current manual
NCBI/Uniprot data below describe general gene information for INHbetaC. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
38,238 Da
NCBI Official Full Name
inhibin beta C chain preproprotein
NCBI Official Synonym Full Names
inhibin, beta C
NCBI Official Symbol
INHBC  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
inhibin beta C chain; activin beta-C chain
UniProt Protein Name
Inhibin beta C chain
UniProt Synonym Protein Names
Activin beta-C chain
Protein Family
UniProt Gene Name
INHBC  [Similar Products]
UniProt Entry Name
INHBC_HUMAN
NCBI Summary for INHbetaC
This gene encodes the beta C chain of inhibin, a member of the TGF-beta superfamily. This subunit forms heterodimers with beta A and beta B subunits. Inhibins and activins, also members of the TGF-beta superfamily, are hormones with opposing actions and are involved in hypothalamic, pituitary, and gonadal hormone secretion, as well as growth and differentiation of various cell types. [provided by RefSeq, Jul 2008]
UniProt Comments for INHbetaC
INHBC: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; hormone activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: regulation of apoptosis; regulation of MAPKKK cascade; cell development; growth
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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