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IOD3 recombinant protein :: Type III iodothyronine deiodinase Recombinant Protein

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Catalog # MBS9422645
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  0.01 mg  /  $230 +1 FREE 8GB USB
  0.05 mg  /  $290 +1 FREE 8GB USB
  0.1 mg  /  $455 +1 FREE 8GB USB
  0.2 mg  /  $705 +1 FREE 8GB USB
  0.5 mg  /  $1,145 +1 FREE 8GB USB
  1 mg  /  $1,800 +2 FREE 8GB USB
IOD3 recombinant protein
Product Name

Type III iodothyronine deiodinase (IOD3), Recombinant Protein

Popular Item
Full Product Name

Recombinant Rat Type III iodothyronine deiodinase

Product Synonym Names
5DIII; DIOIII; Type 3 DI; Type-III 5'-deiodinase
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
: 37-271aa(U132S); Partial, provide the complete extracellular domain.
Sequence
DFQCIRRRVL LTAREESTAE HEDPPLCVSD SNRMCTVESL RAVWHGQKLD YFKSAHLGCS APNTEVVMLE GRRLCKILDF SQGKRPLVVN FGSCTSPPFM ARLQAYRRLA AQH VGIAD FLLVYIEEAH PSDGWLSTDA SYQIPQHQCL QDRLAAAQLM LQGAPGCRVV VDTMDNSSNA AYGAYFERLY IVLEGKVVYQ GGRGPEGYKI SELRMWLEQY QQGLMGTK GSGQVVIQV
3D Structure
ModBase 3D Structure for P49897
Host
Yeast
Species Reactivity
Rat
Purity/Purification
Greater than 90% as determined by SDS-PAGE.
Form/Format
20mM Tris-HCl based buffer, pH8.0
Tag information
EP, YP, BP, MP: Tag type will be determined during the manufacturing process.

The expected tag for each expression system is list as follows:
YP: N-terminal 6xHis-tagged; EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged
Tag Info
N-terminal 6xHis-tagged
Note
The amino acid at position 132 is originally a "U", which has already been mutated to be "S" in the above sequence. This is due to the "U", or selenocysteine, which is encoded by UGA, is normally used as a stop codon in prokaryotic cells. Thus leading to early termination of translation. So we have substituted this "U" to simply be an "S" so that translation can proceed. Changing to cysteine may result in undesired disulfide bond formation, so serine is chosen since it is structurally related to both selenocysteine and cysteine.
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
Other Notes
Small volumes of IOD3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
IOD3 recombinant protein
Responsible for the deiodination of T4 (3, 5, 3', 5'-tetraiodothyronine) into RT3 (3, 3', 5'-triiodothyronine) and of T3 (3, 5, 3'-triiodothyronine) into T2 (3, 3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing prature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during bryological development.
NCBI/Uniprot data below describe general gene information for IOD3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
29.4kD
NCBI Official Full Name
thyroxine 5-deiodinase
NCBI Official Synonym Full Names
iodothyronine deiodinase 3
NCBI Official Symbol
NCBI Official Synonym Symbols
5DIII; DIOIII
  [Similar Products]
NCBI Protein Information
thyroxine 5-deiodinase
UniProt Protein Name
Thyroxine 5-deiodinase
UniProt Synonym Protein Names
5DIII; DIOIII; Type 3 DI; Type III iodothyronine deiodinase
UniProt Gene Name
UniProt Synonym Gene Names
Itdi3; Txdi3  [Similar Products]
NCBI Summary for IOD3
This is an intronless, imprinted gene that is preferentially expressed from the paternal allele. The encoded protein belongs to the iodothyronine deiodinase family, and catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5' triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. It is highly expressed in placenta, fetal and neonatal tissues, and thought to prevent premature exposure of developing fetal tissues to adult levels of thyroid hormones. It thus plays a critical role in mammalian development by regulating circulating fetal thyroid hormone concentration. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jun 2016]
UniProt Comments for IOD3
DIO3: Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development. Belongs to the iodothyronine deiodinase family.

Protein type: EC 1.97.1.11; Membrane protein, integral; Oxidoreductase

Chromosomal Location of Human Ortholog: 6q32

Molecular Function: thyroxine 5'-deiodinase activity; thyroxine 5-deiodinase activity

Biological Process: apoptosis; positive regulation of multicellular organism growth; response to hypoxia; retinal cone cell development; thyroid hormone catabolic process; thyroid hormone metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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