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IP3 elisa kit :: Bovine Inositol Triphosphate ELISA Kit

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Catalog # MBS007776
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
IP3 elisa kit
Product Name

Inositol Triphosphate (IP3), ELISA Kit

Popular Item
Full Product Name

Bovine Inositol Triphosphate ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q63269
Species Reactivity
Specificity
No significant cross-reactivity or interference between Bovine IP3 and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Assay Type
Sandwich
Detection Range
31.2 pg/ml - 1000 pg/ml.
Sensitivity
5.0 pg/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IP3 concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine IP3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IP3 and IP3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IP3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forIP3purchase
MBS007776 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Inositol Triphosphate (IP3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IP3. The ELISA analytical biochemical technique of the MBS007776 kit is based on IP3 antibody-IP3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IP3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IP3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IP3 elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IP3 concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine IP3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IP3 and IP3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS007776. Click to request current manual
NCBI/Uniprot data below describe general gene information for IP3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
304,286 Da
NCBI Official Full Name
inositol triphosphate receptor subtype 3
NCBI Official Synonym Full Names
inositol 1,4,5-trisphosphate receptor, type 3
NCBI Official Symbol
Itpr3  [Similar Products]
NCBI Official Synonym Symbols
IP3R3; IP3R3X
  [Similar Products]
NCBI Protein Information
inositol 1,4,5-trisphosphate receptor type 3; IP3R 3; IP3R-3; insP3R3; IP3 receptor; type 3 InsP3 receptor; inositol 1,4,5-triphosphate receptor 3; inositol 1,4,5-triphosphate receptor, type 3; type 3 inositol 1,4,5-trisphosphate receptor
UniProt Protein Name
Inositol 1,4,5-trisphosphate receptor type 3
UniProt Synonym Protein Names
IP3 receptor isoform 3; IP3R 3; InsP3R3; Type 3 inositol 1,4,5-trisphosphate receptor
UniProt Gene Name
Itpr3  [Similar Products]
UniProt Synonym Gene Names
IP3R 3; InsP3R3  [Similar Products]
UniProt Entry Name
ITPR3_RAT
NCBI Summary for IP3
receptor for Inositol 1,4,5-trisphosphate (IP3); involved in second messenger signaling [RGD, Feb 2006]
UniProt Comments for IP3
IP3R3: a multi-pass endoplasmic reticulum membrane receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. Expressed in intestinal crypt and villus epithelial cells. The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. Phosphorylated on tyrosine residues. Phosphorylated by AKT1 on serine and/or threonine residues. Belongs to the InsP3 receptor family.

Protein type: Membrane protein, integral; Channel, ligand-gated; Membrane protein, multi-pass; Channel, calcium

Cellular Component: nuclear outer membrane; endoplasmic reticulum membrane; endoplasmic reticulum; integral to plasma membrane; dendrite; integral to membrane; nuclear envelope; nucleoplasm; membrane; cell soma; perinuclear region of cytoplasm; apical part of cell; cytoplasm; plasma membrane; nucleolus; nucleus; receptor complex; brush border; myelin sheath

Molecular Function: inositol 1,3,4,5 tetrakisphosphate binding; calcium ion transmembrane transporter activity; inositol hexakisphosphate binding; calcium ion binding; intracellular ligand-gated calcium channel activity; phosphoinositide binding; inositol 1,4,5-triphosphate-sensitive calcium-release channel activity

Biological Process: G-protein coupled receptor protein signaling pathway; sensory perception of umami taste; elevation of cytosolic calcium ion concentration; inositol phosphate-mediated signaling; sensory perception of sweet taste; protein heterooligomerization; calcium ion transport; sensory perception of bitter taste; response to calcium ion; protein homooligomerization; memory
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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