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IRAK1 elisa kit :: Human IRAK1 ELISA Kit

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Catalog # MBS9501345
Unit / Price
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  2x96 Wells  /  $535 +1 FREE 8GB USB
IRAK1 elisa kit
Product Name

IRAK1, ELISA Kit

Popular Item
Full Product Name

Phospho-IRAK1 (Thr209) Colorimetric Cell-Based ELISA Kit

Product Synonym Names
IL1RAK; IRAK; IRAK-1; Interleukin-1 receptor-associated kinase 1; Pelle-like protein kinase; kinase IRAK1; mPLK
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
L76191 mRNA
3D Structure
ModBase 3D Structure for P51617
Species Reactivity
Form/Format
Two 96-Well Plates
Samples
Researchers plate their cell line of choice
Assay Type
Phospho Cell-Based ELISA
Sensitivity
>=5000 cells/well
Reactivity Note
Human: T209
Detection Method
Colorimetric 450 nm
Preparation and Storage
Store at 4 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IRAK1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for IRAK1purchase
MBS9501345 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IRAK1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IRAK1. The ELISA analytical biochemical technique of the MBS9501345 kit is based on IRAK1 antibody-IRAK1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IRAK1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IRAK1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Product Categories/Family for IRAK1 elisa kit
Sample Manual Insert of MBS9501345. Click to request current manual
NCBI/Uniprot data below describe general gene information for IRAK1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
76537
NCBI Official Full Name
interleukin-1 receptor-associated kinase 1 isoform 2
NCBI Official Synonym Full Names
interleukin 1 receptor associated kinase 1
NCBI Official Symbol
IRAK1  [Similar Products]
NCBI Official Synonym Symbols
IRAK; pelle
  [Similar Products]
NCBI Protein Information
interleukin-1 receptor-associated kinase 1
UniProt Protein Name
Interleukin-1 receptor-associated kinase 1
UniProt Gene Name
IRAK1  [Similar Products]
UniProt Synonym Gene Names
IRAK; IRAK-1  [Similar Products]
NCBI Summary for IRAK1
This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for IRAK1
IRAK1: a TKL kinase of the IRAK family. Involved in Toll/IL-1 signaling. One of two putative serine/threonine kinases that become associated with the interleukin-1 receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Extensively phosphorylated after its association with IL1-R-1. Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Three isoforms of the human protein and produced by alternative splicing. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

Protein type: EC 2.7.11.1; IRAK family; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: cytoplasm; cytosol; endosome membrane; interleukin-1 receptor complex; lipid droplet; nucleus; plasma membrane

Molecular Function: ATP binding; heat shock protein binding; kinase activity; NF-kappaB-inducing kinase activity; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase activity; protein serine/threonine kinase activity

Biological Process: activation of MAPK activity; activation of NF-kappaB-inducing kinase; aging; cellular response to heat; cellular response to hypoxia; JNK cascade; lipopolysaccharide-mediated signaling pathway; MyD88-dependent toll-like receptor signaling pathway; negative regulation of apoptosis; negative regulation of NF-kappaB transcription factor activity; nucleotide-binding oligomerization domain containing signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of interferon type I production; positive regulation of MAP kinase activity; positive regulation of NF-kappaB transcription factor activity; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-templated; protein amino acid phosphorylation; protein autophosphorylation; protein oligomerization; regulation of cytokine-mediated signaling pathway; response to lipopolysaccharide; signal transduction; toll-like receptor 2 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transmembrane receptor protein serine/threonine kinase signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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