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IRF3 recombinant protein :: Interferon regulatory factor-3 Recombinant Protein

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Catalog # MBS203096
Unit / Price
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  0.1 mg  /  $310 +1 FREE 8GB USB
  0.5 mg  /  $720 +1 FREE 8GB USB
SDS-Page
Product Name

Interferon regulatory factor-3 (IRF3), Recombinant Protein

Full Product Name

Interferon regulatory factor-3, 1-112aa, Human, E Coli

Product Synonym Names
IRF3; Interferon regulatory factor-3; IRF-3; Interferon regulatory factor 3; IRF 3.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MGTPKPRILP WLVSQLDLGQ LEGVAWVNKS RTRFRIPWKH GLRQDAQQED FGIFQAWAEA TGAYVPGRDK PDLPTWKRNF RSALNRKEGL RLAEDRSKDP HDPHKIYEFV NS
OMIM
Z56281 mRNA
3D Structure
ModBase 3D Structure for Q14653
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4)
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Antigen Species
Human
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of IRF3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
IRF3 recombinant protein
Members of the Interferon regulatory factor (IRF) family regulate gene expression critical to immune response, hemopoiesis, and proliferation. IRF-3 is a member of the IRF family, and is distinct from other family members. Its transcriptional activity is regulated solely by posttranslational modifications. It plays a crucial role in activation of innate immunity and inflammation in response to viral infection. Recombinant human IRF3 was expressed in E Coli and purified by using conventional chromatography techniques
Product Categories/Family for IRF3 recombinant protein
Applications Tested/Suitable for IRF3 recombinant protein
SDS-PAGE

NCBI/Uniprot data below describe general gene information for IRF3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
13.0kDa (112aa), confirmed by MALDI-TOF. [Similar Products]
NCBI Official Full Name
interferon regulatory factor 3 isoform 1
NCBI Official Synonym Full Names
interferon regulatory factor 3
NCBI Official Symbol
NCBI Official Synonym Symbols
IIAE7
  [Similar Products]
NCBI Protein Information
interferon regulatory factor 3
UniProt Protein Name
Interferon regulatory factor 3
UniProt Gene Name
UniProt Synonym Gene Names
IRF-3  [Similar Products]
NCBI Summary for IRF3
This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
UniProt Comments for IRF3
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
Product References and Citations for IRF3 recombinant protein
Pietila TE et al., J Immunol. (2007) 178 (1):253-61; DeFilippis VR et al., J Virol., (2006) 80 (2):1032-7;

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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