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anti-IRS-1 (Paired S312) antibody :: Rabbit IRS-1 (Paired S312) Antibody

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Catalog # MBS462058
Unit / Price
  0.1 mg  /  $285 +1 FREE 8GB USB
Testing Data
Product Name

IRS-1 (Paired S312), Antibody

Also Known As

Rabbit anti IRS-1 (Paired 312) antibody

Product Synonym Names
Rabbit anti-IRS-1 (Paired S312); Anti-IRS-1 (Paired312); Insulin-receptor substrate 1 (IRS-1).
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 2; NC_000002.11 (227596033..227663506, complement). Location: 2q36
OMIM
125853
3D Structure
ModBase 3D Structure for P35568
Host
Rabbit
Species Reactivity
Human, Rat, Mouse
Specificity
This antibody recognizes ~165 kDa of human IRS-1 (without phosphorylation at Ser312). It also reacts with mouse and rat. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (lot specific)
Antigen Preparation
A synthetic peptide corresponding to the epitope ATSPA without phosphorylation of human IRS-1.
Positive Control
CHO cell lysate
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Other Notes
Small volumes of anti-IRS-1 (Paired S312) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-IRS-1 (Paired S312) antibody
Insulin Receptor Substrate-1 (IRS-1), 165 kDa cytoplasmic docking protein, is one of the major endogenous substrates of the insulin receptor kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain containing proteins, which mediate the metabolic and growth promoting functions of insulin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser312 of IRS-1 is phosphorylated by JNK and IKK and Ser789 is phosphorylated by SIK-2, a member of AMPK family. The phosphorylation of Tyr612 and Ser636/639 is mediated by the PKC and mTOR pathways, respectively and phosphorylation at Ser1101 is mediated by PKC, resulting in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity.
Applications Tested/Suitable for anti-IRS-1 (Paired S312) antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes for anti-IRS-1 (Paired S312) antibody
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 0.5-2 ug/ml

NCBI/Uniprot data below describe general gene information for IRS-1 (Paired S312). It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
0.5-2 ug/ml
NCBI Official Full Name
Insulin receptor substrate 1
NCBI Official Synonym Full Names
insulin receptor substrate 1
NCBI Official Symbol
NCBI Official Synonym Symbols
HIRS-1
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NCBI Protein Information
insulin receptor substrate 1; IRS-1; OTTHUMP00000164234
UniProt Protein Name
Insulin receptor substrate 1
UniProt Gene Name
UniProt Entry Name
IRS1_HUMAN
NCBI Summary for IRS-1 (Paired S312)
This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq]
UniProt Comments for IRS-1 (Paired S312)
Function: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit

By similarity. Ref.28

Subunit structure: Interacts with UBTF and PIK3CA

By similarity. Interacts (via phosphorylated YXXM motifs) with PIK3R1

By similarity. Interacts with ROCK1

By similarity. Interacts (via PH domain) with PHIP

By similarity. Interacts with GRB2

By similarity. Interacts with SOCS7. Interacts (via IRS-type PTB domain) with IGF1R and INSR (via the tyrosine-phosphorylated NPXY motif). Ref.5 Ref.6 Ref.7 Ref.9

Post-translational modification: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor

By similarity.Phosphorylation of Tyr-896 is required for GRB2-binding

Polymorphism: The Arg-971 polymorphism impairs the ability of insulin to stimulate glucose transport, glucose transporter translocation, and glycogen synthesis by affecting the PI3K/AKT1/GSK3 signaling pathway. The polymorphism at Arg-971 may contribute to the in vivo insulin resistance observed in carriers of this variant. Arg-971 could contribute to the risk for atherosclerotic cardiovascular diseases associated with non-insulin-dependent diabetes mellitus (NIDDM) by producing a cluster of insulin resistance-related metabolic abnormalities. In insulin-stimulated human endothelial cells from carriers of the Arg-971 polymorphism, genetic impairment of the IRS1/PI3K/PDPK1/AKT1 insulin signaling cascade results in impaired insulin-stimulated nitric oxide (NO) release and suggested that this may be a mechanism through which the Arg-971 polymorphism contributes to the genetic predisposition to develop endothelial dysfunction and cardiovascular disease. The Arg-971 polymorphism not only reduces phosphorylation of the substrate but allows IRS1 to act as an inhibitor of PI3K, producing global insulin resistance.

Involvement in disease: Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [

MIM:125853]. Ref.22 Ref.23 Ref.25 Ref.28 Ref.29

Sequence similarities: Contains 1 IRS-type PTB domain.Contains 1 PH domain.
Product References and Citations for anti-IRS-1 (Paired S312) antibody
Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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