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anti-IRS-2 (pS731) antibody :: Rabbit IRS-2 (pS731) Antibody

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Catalog # MBS462062
Unit / Price
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  0.1 mg  /  $285 +1 FREE 8GB USB
Testing Data
Product Name

IRS-2 (pS731), Antibody

Popular Item
Full Product Name

Rabbit anti Phospho- IRS-2 (pSer 731) antibody

Product Synonym Names
Rabbit anti-IRS-2 (p Ser731); Anti-phospho-IRS-2 (pSer731); Insulin-receptor substrate 2 (IRS-2).
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 13; NC_000013.10 (110406184..110438914, complement). Location: 13q34
OMIM
600797
3D Structure
ModBase 3D Structure for Q96RG4
Host
Rabbit
Species Reactivity
Human, Rat, Mouse
Specificity
This antibody recognizes ~180 kDa of human IRS-2 (pSer731). It also reacts with mouse and rat. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (lot specific)
Antigen Preparation
A synthetic peptide corresponding to the epitope ASSPA with a single phosphorylation site Ser 731 of human IRS-2.
Positive Control
3T3 cell lysate
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Other Notes
Small volumes of anti-IRS-2 (pS731) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-IRS-2 (pS731) antibody
Insulin Receptor Substrate-2 (IRS-2), a 180kDa cytoplasmic docking protein, is one of the major endogenous substrates of the insulin receptor kinase. The insulin receptor mediates insulin action through the phosphorylation of substrate proteins. The major substrates of the insulin receptor kinase are the insulin receptor substrate (IRS) family of proteins, IRS 1, IRS 2, IRS 3, and IRS 4. IRS 2, a signaling molecule that mediates effects of insulin, Insulin-like growth factor 1 and other cytokines by acting as a molecular adaptor between diverse receptor kinases and downstream effectors. IRS proteins contain more than 70 potential Ser / Thr phosphorylation sites for kinases like PKA (cAMP-dependent protein kinase), PKC, and MAPK. The phosphorylation of Ser / Thr residues of IRS proteins has a dual function, serving either for a positive or negative modulation of insulin signal transduction.
Applications Tested/Suitable for anti-IRS-2 (pS731) antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes for anti-IRS-2 (pS731) antibody
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 0.5-2 ug/ml

anti-IRS-2 (pS731) antibody Testing Data image
NCBI/Uniprot data below describe general gene information for IRS-2 (pS731). It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
180 kDa
NCBI Official Full Name
insulin receptor substrate 2
NCBI Official Synonym Full Names
insulin receptor substrate 2
NCBI Official Symbol
NCBI Official Synonym Symbols
IRS-2
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NCBI Protein Information
insulin receptor substrate 2; OTTHUMP00000018696
UniProt Protein Name
Insulin receptor substrate 2 insertion mutant
UniProt Gene Name
UniProt Entry Name
Q96RG4_HUMAN
NCBI Summary for IRS-2 (pS731)
This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq]
UniProt Comments for IRS-2 (pS731)
Sequence similarities: Contains 1 PH domain. RuleBase RU003350
Product References and Citations for anti-IRS-2 (pS731) antibody
Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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