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ISG15 recombinant protein :: Ubiquitin-like protein ISG15 Recombinant Protein

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Catalog # MBS717412
Unit / Price
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  0.01 mg  /  $160 +1 FREE 8GB USB
  0.05 mg  /  $200 +1 FREE 8GB USB
  0.1 mg  /  $295 +1 FREE 8GB USB
  0.2 mg  /  $480 +1 FREE 8GB USB
  0.5 mg  /  $790 +1 FREE 8GB USB
  1 mg  /  $1,215 +1 FREE 8GB USB
SDS-PAGE
Product Name

Ubiquitin-like protein ISG15, Recombinant Protein

Popular Item
Full Product Name

Recombinant Human Ubiquitin-like protein ISG15

Product Synonym Names
Interferon-induced 15 kDa proteinInterferon-induced 17 kDa protein; IP17Ubiquitin cross-reactive protein
Product Synonym Gene Name
G1P2; UCRP[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS717412 COA
Sequence Positions
2-157. Full length.
Sequence
GWDLTVKMLA GNEFQVSLSS SMSVSELKAQ ITQKIGVHAF QQRLAVHPSG VALQDRVPLA SQGLGPGSTV LLVVDKCDEP LSILVRNNKG RSSTYEVRLT QTVAHLKQQV SGLEGVQDDL FWLTFEGKPL EDQLPLGEYG LKPLSTVFMN LRLRGG
3D Structure
ModBase 3D Structure for Q64339
Host
E Coli
Purity/Purification
Greater than 90% as determined by SDS-PAGE. (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of ISG15 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ISG15 recombinant protein
Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Its target proteins include SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, TRIM25, STAT5A, MAPK1/ERK2 and globin. Can also isgylate: DDX58/RIG-I which inhibits its function in antiviral signaling response and EIF4E2 which enhances its cap structure-binding activity and translation-inhibition activity. Exhibits antiviral activity towards both DNA and RNA viruses, including influenza A and B virus, sindbis virus (SV) and herpes simplex type-1 (HHV-1). Plays a significant role in the control of neonatal Chikungunya virus (CHIKV) infection by acting as a putative immunomodulator of proinflammatory cytokines. Protects mice against the consequences of Chikungunya virus infection by downregulating the pathogenic cytokine response, often denoted as the cytokine storm. Plays a role in erythroid differentiation. The secreted form of ISG15 can: induce natural killer cell proliferation, act as a chotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity
Product Categories/Family for ISG15 recombinant protein

ISG15 recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
NCBI/Uniprot data below describe general gene information for ISG15. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
44.4kD
NCBI Official Full Name
ubiquitin-like protein ISG15
NCBI Official Synonym Full Names
ISG15 ubiquitin-like modifier
NCBI Official Symbol
Isg15  [Similar Products]
NCBI Official Synonym Symbols
G1p2; IP17; Irfp; UCRP; IGI15
  [Similar Products]
NCBI Protein Information
ubiquitin-like protein ISG15
UniProt Protein Name
Ubiquitin-like protein ISG15
UniProt Synonym Protein Names
Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; IP17; Ubiquitin cross-reactive protein
Protein Family
UniProt Gene Name
Isg15  [Similar Products]
UniProt Synonym Gene Names
G1p2; Ucrp; IP17  [Similar Products]
UniProt Entry Name
ISG15_MOUSE
UniProt Comments for ISG15
ISG15: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interaction with influenza B NS1 protein inhibits this conjugation. By type I interferons. Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.

Protein type: Ubiquitin-like modifier

Cellular Component: cytoplasm; extracellular region

Molecular Function: protein binding; protein tag

Biological Process: defense response to bacterium; defense response to virus; ISG15-protein conjugation; modification-dependent protein catabolic process; negative regulation of protein ubiquitination; negative regulation of viral genome replication; positive regulation of erythrocyte differentiation; regulation of interferon-gamma production
Product References and Citations for ISG15 recombinant protein
Nucleotide sequence and deduced amino acid sequence of a cDNA encoding an interferon-induced mouse 15-KDa protein.Fahey D. Sequence differences between murine ISG15, human ISG15 and ubiquitin a functional divergence from ubiquitin.Garlie N.K., Haas A.L., D'Cunha J., Schilling D., Knight E. Jr., Borden E.C. Serpin 2a is induced in activated macrophages and conjugates to a ubiquitin homolog.Hamerman J.A., Hayashi F., Schroeder L.A., Gygi S.P., Haas A.L., Hampson L., Coughlin P., Aebersold R., Aderem A.J. Immunol. 168:2415-2423(2002) Identification of a ubiquitin family protein as a novel neutrophil chemotactic factor.Owhashi M., Taoka Y., Ishii K., Nakazawa S., Uemura H., Kambara H.Biochem. Biophys. Res. Commun. 309:533-539(2003) High-throughput immunoblotting. Ubiquitin-like protein ISG15 modifies key regulators of signal transduction.Malakhov M.P., Kim K.I., Malakhova O.A., Jacobs B.S., Borden E.C., Zhang D.-E.J. Biol. Chem. 278:16608-16613(2003) Identification of interferon-stimulated gene 15 as an antiviral molecule during Sindbis virus infection in vivo.Lenschow D.J., Giannakopoulos N.V., Gunn L.J., Johnston C., O'Guin A.K., Schmidt R.E., Levine B., Virgin H.W. IVJ. Virol. 79:13974-13983(2005) Negative regulation of ISG15 E3 ligase EFP through its autoISGylation.Zou W., Wang J., Zhang D.-E.Biochem. Biophys. Res. Commun. 354:321-327(2007) ISG15 modification of the eIF4E cognate 4EHP enhances cap structure-binding activity of 4EHP.Okumura F., Zou W., Zhang D.E.Genes Dev. 21:255-260(2007) IFN-stimulated gene 15 functions as a critical antiviral molecule against influenza, herpes, and Sindbis viruses.Lenschow D.J., Lai C., Frias-Staheli N., Giannakopoulos N.V., Lutz A., Wolff T., Osiak A., Levine B., Schmidt R.E., Garcia-Sastre A., Leib D.A., Pekosz A., Knobeloch K.P., Horak I., Virgin H.W. IVProc. Natl. Acad. Sci. U.S.A. 104:1371-1376(2007) Nitrosylation of ISG15 prevents the disulfide bond-mediated dimerization of ISG15 and contributes to effective ISGylation.Okumura F., Lenschow D.J., Zhang D.E.J. Biol. Chem. 283:24484-24488(2008) Negative feedback regulation of RIG-I-mediated antiviral signaling by interferon-induced ISG15 conjugation.Kim M.J., Hwang S.Y., Imaizumi T., Yoo J.Y.J. Virol. 82:1474-1483(2008) Emerging role of ISG15 in antiviral immunity.Skaug B., Chen Z.J.Cell 143:187-190(2010) ISG15 modulates development of the erythroid lineage.Maragno A.L., Pironin M., Alcalde H., Cong X., Knobeloch K.P., Tangy F., Zhang D.E., Ghysdael J., Quang C.T.PLoS ONE 6:E26068-E26068(2011) ISG15 is critical in the control of Chikungunya virus infection independent of UbE1L mediated conjugation.Werneke S.W., Schilte C., Rohatgi A., Monte K.J., Michault A., Arenzana-Seisdedos F., Vanlandingham D.L., Higgs S., Fontanet A., Albert M.L., Lenschow D.J.PLoS Pathog. 7:E1002322-E1002322(2011) Mycobacterial disease and impaired IFN-gamma immunity in humans with inherited ISG15 deficiency.Bogunovic D., Byun M., Durfee L.A., Abhyankar A., Sanal O., Mansouri D., Salem S., Radovanovic I., Grant A.V., Adimi P., Mansouri N., Okada S., Bryant V.L., Kong X.F., Kreins A., Velez M.M., Boisson B., Khalilzadeh S., Ozcelik U., Darazam I.A., Schoggins J.W., Rice C.M., Al-Muhsen S., Behr M., Vogt G., Puel A., Bustamante J., Gros P., Huibregtse J.M., Abel L., Boisson-Dupuis S., Casanova J.L.Science 337:1684-1688(2012)

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Organs/Tissues associated with ISG15 recombinant protein
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >28 publications with ISG15 and Brain
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