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ITGA5 elisa kit :: Human integrin, alpha 5 (fibronectin receptor, alpha polypeptide) ELISA Kit

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Catalog # MBS705816
Unit / Price
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Typical Testing Data/Standard Curve (for reference only)
Product Name

integrin, alpha 5 (fibronectin receptor, alpha polypeptide) (ITGA5), ELISA Kit

Popular Item
Full Product Name

Human Integrin alpha-5, ITGA5 ELISA Kit

Product Synonym Names
Human Integrin alpha-5 (ITGA5) Elisa kit; CD49e; FNRA; VLA5A; fibronectin receptor; alpha subunit; integrin alpha 5; very late activation protein 5; alpha subunit; integrin; alpha 5 (fibronectin receptor; alpha polypeptide)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS705816 Testing Data
OMIM
135620
3D Structure
ModBase 3D Structure for P08648
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human ITGA5. No significant cross-reactivity or interference between Human ITGA5 and analogues was observed.
Samples
Serum, plasma, cell culture supernates, urine and cerebrospinal fluid (CSF)
Assay Type
Sandwich
Detection Range
0.312 ng/ml-20 ng/ml
Sensitivity
0.078 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Cell Adhesion
Protein Biological Process 3
Cell adhesion
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ITGA5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forITGA5purchase
MBS705816 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the integrin, alpha 5 (fibronectin receptor, alpha polypeptide) (ITGA5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ITGA5. The ELISA analytical biochemical technique of the MBS705816 kit is based on ITGA5 antibody-ITGA5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ITGA5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ITGA5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ITGA5 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ITGA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ITGA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ITGA5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ITGA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of ITGA5 elisa kit
ITGA5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS705816. Click to request current manual
NCBI/Uniprot data below describe general gene information for ITGA5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
114,536 Da
NCBI Official Full Name
integrin alpha-5
NCBI Official Synonym Full Names
integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
NCBI Official Symbol
ITGA5  [Similar Products]
NCBI Official Synonym Symbols
FNRA; CD49e; VLA5A
  [Similar Products]
NCBI Protein Information
integrin alpha-5; VLA-5; integrin alpha-F; CD49 antigen-like family member E; fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; very late activation protein 5, alpha subunit
UniProt Protein Name
Integrin alpha-5
UniProt Synonym Protein Names
CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; VLA-5; CD_antigen: CD49eCleaved into the following 2 chains:Integrin alpha-5 heavy chain; Integrin alpha-5 light chain
Protein Family
UniProt Gene Name
ITGA5  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
ITA5_HUMAN
NCBI Summary for ITGA5
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq, Jul 2008]
UniProt Comments for ITGA5
Function: Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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