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ITIH3 elisa kit :: Human Inter-alpha-trypsin inhibitor heavy chain H3 ELISA Kit

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Catalog # MBS2885234
Unit / Price
  48-Strip-Wells  /  $400 +1 FREE 8GB USB
  96-Strip-Wells  /  $530 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,305 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $4,250 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Inter-alpha-trypsin inhibitor heavy chain H3 (ITIH3), ELISA Kit

Popular Item
Also Known As

Human Inter-alpha-trypsin inhibitor heavy chain H3 ELISA Kit

Product Synonym Names
Inter-alpha-trypsin inhibitor heavy chain H3; ITI heavy chain H3; ITI-HC3; Inter-alpha-inhibitor heavy chain 3; Serum-derived hyaluronan-associated protein; SHAP; ITIH3
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2885234 Testing Data
Sequence Length
890
OMIM
146650
3D Structure
ModBase 3D Structure for Q06033
Species Reactivity
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ITIH3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forITIH3purchase
MBS2885234 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Inter-alpha-trypsin inhibitor heavy chain H3 (ITIH3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ITIH3. The ELISA analytical biochemical technique of the MBS2885234 kit is based on ITIH3 antibody-ITIH3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ITIH3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ITIH3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
ITIH3 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of ITIH3 elisa kit
ITIH3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2885234. Click to request current manual
NCBI/Uniprot data below describe general gene information for ITIH3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
99,328 Da
NCBI Official Full Name
inter-alpha-trypsin inhibitor heavy chain H3 preproprotein
NCBI Official Synonym Full Names
inter-alpha-trypsin inhibitor heavy chain 3
NCBI Official Symbol
ITIH3  [Similar Products]
NCBI Official Synonym Symbols
H3P; SHAP; ITI-HC3
  [Similar Products]
NCBI Protein Information
inter-alpha-trypsin inhibitor heavy chain H3
UniProt Protein Name
Inter-alpha-trypsin inhibitor heavy chain H3
UniProt Synonym Protein Names
Serum-derived hyaluronan-associated protein; SHAP
UniProt Gene Name
ITIH3  [Similar Products]
UniProt Synonym Gene Names
ITI heavy chain H3; ITI-HC3; Inter-alpha-inhibitor heavy chain 3; SHAP  [Similar Products]
UniProt Entry Name
ITIH3_HUMAN
NCBI Summary for ITIH3
This gene encodes the heavy chain subunit of the pre-alpha-trypsin inhibitor complex. This complex may stabilize the extracellular matrix through its ability to bind hyaluronic acid. Polymorphisms of this gene may be associated with increased risk for schizophrenia and major depressive disorder. This gene is present in an inter-alpha-trypsin inhibitor family gene cluster on chromosome 3. [provided by RefSeq, Jul 2015]
UniProt Comments for ITIH3
ITIH3: May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes. Belongs to the ITIH family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Inhibitor

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: extracellular region

Molecular Function: endopeptidase inhibitor activity; serine-type endopeptidase inhibitor activity

Biological Process: hyaluronan metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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