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anti-JAK2 antibody :: JAK2 Antibody

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Catalog # MBS852339
Unit / Price
  0.1 mg  /  $215 +1 FREE 8GB USB
Western Blot (WB)
Product Name

JAK2, Antibody

Popular Item
Full Product Name

JAK2 (Phospho-Tyr570) Antibody

Product Synonym Names
EC 2.7.10.2; JAK-2; JAK2; Janus kinase 2; kinase Jak2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
147796
3D Structure
ModBase 3D Structure for O60674
Species Reactivity
Human, Mouse, Rat
Specificity
JAK2 (Phospho-Tyr570) antibody detects endogenous levels of JAK2 only when phosphorylated at tyrosine 750.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human JAK2 around the phosphorylation site of tyrosine 750 (G-D-YP-G-Q).
All Sites
Human: Tyr750
Mouse: Tyr750
Rat: Tyr750
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-JAK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-JAK2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-JAK2 antibody
WB: 1:500-1:1000
ELISA: 1:1000

Western Blot (WB) of anti-JAK2 antibody
Western blot analysis of extracts from 293 cells, treated with etoposide (25uM, 24hours), using JAK2 (Phospho-Tyr570) antibody.
anti-JAK2 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for JAK2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
130,674 Da
NCBI Official Full Name
tyrosine-protein kinase JAK2 isoform a
NCBI Official Synonym Full Names
Janus kinase 2
NCBI Official Symbol
NCBI Official Synonym Symbols
JTK10; THCYT3
  [Similar Products]
NCBI Protein Information
tyrosine-protein kinase JAK2
UniProt Protein Name
Tyrosine-protein kinase JAK2
UniProt Synonym Protein Names
Janus kinase 2; JAK-2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
JAK-2  [Similar Products]
NCBI Summary for JAK2
This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008]
UniProt Comments for JAK2
Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins (PubMed:7615558). Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins (PubMed:9618263). Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B) (PubMed:21368206). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation (PubMed:20098430). Plays a role in cell cycle by phosphorylating CDKN1B (PubMed:21423214). Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin (PubMed:19783980).
Product References and Citations for anti-JAK2 antibody
Saltzman A., Biochem. Biophys. Res. Commun. 246:627-633(1998). Dalal I., Blood 91:844-851(1998). Peeters P., Blood 90:2535-2540(1997).

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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