• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

KARS elisa kit :: Mouse Lysyl tRNA Synthetase (KARS) ELISA Kit

Scan QR to view Datasheet
Catalog # MBS2023685 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $270 +1 FREE 8GB USB
  48-Strip-Wells  /  $505 +1 FREE 8GB USB
  96-Strip-Wells  /  $685 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,770 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,025 +6 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Lysyl tRNA Synthetase (KARS), ELISA Kit

Popular Item
Also Known As

Lysyl tRNA Synthetase (KARS) ELISA Kit

Product Synonym Names
KARS2; LysRS; Lysine tRNA Ligase
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
625
OMIM
601421
3D Structure
ModBase 3D Structure for Q15046
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Lysyl tRNA Synthetase (KARS).
No significant cross-reactivity or interference between Lysyl tRNA Synthetase (KARS) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates and Other Biological Fluids
Detection Range
31.2-2000pg/mL
Sensitivity
< 12.9pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Lysyl tRNA Synthetase (KARS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Lysyl tRNA Synthetase (KARS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of KARS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forKARSpurchase
MBS2023685 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lysyl tRNA Synthetase (KARS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KARS. The ELISA analytical biochemical technique of the MBS2023685 kit is based on KARS antibody-KARS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KARS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KARS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
KARS elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysyl tRNA Synthetase (KARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysyl tRNA Synthetase (KARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysyl tRNA Synthetase (KARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysyl tRNA Synthetase (KARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for KARS elisa kit

Typical Standard Curve/Testing Data of KARS elisa kit
KARS elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS2023685. Click to request current manual
NCBI/Uniprot data below describe general gene information for KARS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
71,497 Da
NCBI Official Full Name
Lysyl tRNA Synthetase
NCBI Official Synonym Full Names
lysyl-tRNA synthetase
NCBI Official Symbol
NCBI Official Synonym Symbols
KRS; KARS1; KARS2; CMTRIB; DFNB89
  [Similar Products]
NCBI Protein Information
lysine--tRNA ligase
UniProt Protein Name
Lysine--tRNA ligase
UniProt Synonym Protein Names
Lysyl-tRNA synthetase; LysRS
UniProt Gene Name
UniProt Synonym Gene Names
KIAA0070; LysRS  [Similar Products]
UniProt Entry Name
SYK_HUMAN
NCBI Summary for KARS
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for KARS
KARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. Homodimer; also part of a multisubunit complex that groups AIMP1, AIMP2, EEF1A1 and tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro. Interacts with AIMP2 (via N- terminus) and MITF. Interacts directly with HIV-1 virus GAG protein. Up-regulated by DARS and EEF1A1, but not by AIMP2. Belongs to the class-II aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; Mitochondrial; Ligase; EC 6.1.1.6

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: microtubule cytoskeleton; aminoacyl-tRNA synthetase multienzyme complex; mitochondrion; mitochondrial matrix; cytoplasm; plasma membrane; extracellular region; cytosol; nucleus

Molecular Function: amino acid binding; protein binding; metal ion binding; lysine-tRNA ligase activity; ATP binding; tRNA binding

Biological Process: tRNA aminoacylation for protein translation; viral reproduction; lysyl-tRNA aminoacylation; tRNA processing; gene expression; diadenosine tetraphosphate biosynthetic process

Disease: Deafness, Autosomal Recessive 89; Charcot-marie-tooth Disease, Recessive Intermediate B
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000