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KARSL elisa kit :: Mouse Lysyl tRNA Synthetase ELISA Kit

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Catalog # MBS2533484
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Lysyl tRNA Synthetase (KARSL), ELISA Kit

Popular Item
Also Known As

Mouse KARSL (Lysyl tRNA Synthetase) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
597
OMIM
601421
Species Reactivity
Specificity
This kit recognizes natural and recombinantMouseKARS. No significant cross-reactivity or interference between MouseKARS and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
62.5-4000pg/mL
Sensitivity
Min: 37.5pg/mL; Max: 4000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of KARSL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forKARSLpurchase
MBS2533484 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lysyl tRNA Synthetase (KARSL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KARSL. The ELISA analytical biochemical technique of the MBS2533484 kit is based on KARSL antibody-KARSL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KARSL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KARSL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
KARSL elisa kit
Intended Uses: This ELISA kit applies to the invitro quantitative determination of MouseKARSconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to KARS. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for KARSand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain KARS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of KARS.You can calculate the concentration of KARSin the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of KARSL elisa kit
KARSL elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2533484. Click to request current manual
NCBI/Uniprot data below describe general gene information for KARSL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
71,497 Da
NCBI Official Full Name
Lysyl tRNA Synthetase
NCBI Official Synonym Full Names
lysyl-tRNA synthetase
NCBI Official Symbol
NCBI Official Synonym Symbols
KRS; KARS1; KARS2; CMTRIB; DFNB89
  [Similar Products]
NCBI Protein Information
lysine--tRNA ligase
UniProt Protein Name
Lysine--tRNA ligase
UniProt Synonym Protein Names
Lysyl-tRNA synthetase; LysRS
UniProt Gene Name
UniProt Synonym Gene Names
KIAA0070; LysRS  [Similar Products]
UniProt Entry Name
SYK_HUMAN
NCBI Summary for KARSL
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for KARSL
KARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. Homodimer; also part of a multisubunit complex that groups AIMP1, AIMP2, EEF1A1 and tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro. Interacts with AIMP2 (via N- terminus) and MITF. Interacts directly with HIV-1 virus GAG protein. Up-regulated by DARS and EEF1A1, but not by AIMP2. Belongs to the class-II aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 6.1.1.6; Translation; Ligase

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: aminoacyl-tRNA synthetase multienzyme complex; cytoplasm; cytosol; extracellular region; microtubule cytoskeleton; mitochondrial matrix; mitochondrion; nucleus; plasma membrane

Molecular Function: amino acid binding; ATP binding; lysine-tRNA ligase activity; metal ion binding; protein binding; tRNA binding

Biological Process: diadenosine tetraphosphate biosynthetic process; gene expression; lysyl-tRNA aminoacylation; selenium metabolic process; tRNA aminoacylation for protein translation; tRNA processing; viral reproduction

Disease: Charcot-marie-tooth Disease, Recessive Intermediate B; Deafness, Autosomal Recessive 89
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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