• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

KCNH2 elisa kit :: Human Potassium voltage gated channel subfamily H member 2 (KCNH2) ELISA Kit

Scan QR to view Datasheet
Catalog # MBS7221798
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Potassium voltage gated channel subfamily H member 2 (KCNH2), ELISA Kit

Popular Item
Also Known As

Human Potassium voltage gated channel subfamily H member 2 (KCNH2) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
613688
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of KCNQ2. No significant cross-reactivity or interference between KCNQ2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between KCNQ2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of KCNH2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forKCNH2purchase
MBS7221798 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Potassium voltage gated channel subfamily H member 2 (KCNH2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KCNH2. The ELISA analytical biochemical technique of the MBS7221798 kit is based on KCNH2 antibody-KCNH2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KCNH2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KCNH2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
KCNH2 elisa kit
Intended Uses: This KCNQ2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human KCNQ2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: KCNQ2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-KCNQ2 antibody and an KCNQ2-HRP conjugate. The assay sample and buffer are incubated together with KCNQ2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KCNQ2 concentration since KCNQ2 from samples and KCNQ2-HRP conjugate compete for the anti-KCNQ2 antibody binding site. Since the number of sites is limited, as more sites are occupied by KCNQ2 from the sample, fewer sites are left to bind KCNQ2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KCNQ2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for KCNH2 elisa kit

Typical Testing Data/Standard Curve (for reference only) of KCNH2 elisa kit
KCNH2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7221798. Click to request current manual
NCBI/Uniprot data below describe general gene information for KCNH2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
115,636 Da
NCBI Official Full Name
Potassium voltage-gated channel subfamily H member 2
NCBI Official Synonym Full Names
potassium voltage-gated channel, subfamily H (eag-related), member 2
NCBI Official Symbol
KCNH2  [Similar Products]
NCBI Official Synonym Symbols
ERG1; HERG; LQT2; SQT1; ERG-1; H-ERG; HERG1; Kv11.1
  [Similar Products]
NCBI Protein Information
potassium voltage-gated channel subfamily H member 2; eag homolog; eag-related protein 1; ether-a-go-go-related protein 1; ether-a-go-go-related gene potassium channel 1; voltage-gated potassium channel subunit Kv11.1; ether-a-go-go-related potassium channel protein
UniProt Protein Name
Potassium voltage-gated channel subfamily H member 2
UniProt Synonym Protein Names
Eag homolog; Ether-a-go-go-related gene potassium channel 1; ERG-1; Eag-related protein 1; Ether-a-go-go-related protein 1; H-ERG; hERG-1; hERG1; Voltage-gated potassium channel subunit Kv11.1
UniProt Gene Name
KCNH2  [Similar Products]
UniProt Synonym Gene Names
ERG; ERG1; HERG; ERG-1; Eag-related protein 1; Ether-a-go-go-related protein 1; H-ERG; hERG-1; hERG1  [Similar Products]
UniProt Entry Name
KCNH2_HUMAN
NCBI Summary for KCNH2
This gene encodes a voltage-activated potassium channel belonging to the eag family. It shares sequence similarity with the Drosophila ether-a-go-go (eag) gene. Mutations in this gene can cause long QT syndrome type 2 (LQT2). Transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Comments for KCNH2
Kv11.1: the ether-a-go-go related gene is a pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel is associated with cardiac arrhythmias and rhythmic excitability of the pituitary. Channel properties are modulated by cAMP and subunit assembly. Mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr). The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimer with Kv11.2 and Kv11.3. Interacts with ALG10B. Heteromultimer with KCNE1 and KCNE2. Defects in Kv11.1 are the cause of long QT syndrome type 2 (LQT2), a heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. Deafness is often associated with LQT2. Defects in Kv11.1 are the cause of short QT syndrome type 1 (SQT1), a heart disorder characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death. Four isoforms of the human protein are produced by alternative splicing. Isoform 3 has no channel activity by itself, but modulates channel characteristics when associated with isoform 1.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Channel, potassium

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: voltage-gated potassium channel complex; cell surface; cytoplasm; plasma membrane; nuclear envelope

Molecular Function: identical protein binding; voltage-gated potassium channel activity; protein binding; protein homodimerization activity; delayed rectifier potassium channel activity; ubiquitin protein ligase binding; inward rectifier potassium channel activity; two-component sensor activity

Biological Process: synaptic transmission; regulation of membrane potential; two-component signal transduction system (phosphorelay); potassium ion homeostasis; regulation of the rate of heart contraction by hormone; cardiac muscle contraction

Disease: Long Qt Syndrome 2; Short Qt Syndrome 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000