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KCNJ11 elisa kit :: Human ATP sensitive inward rectifier potassium channel 11 (KCNJ11) ELISA Kit

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Catalog # MBS7224583
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KCNJ11 elisa kit
Product Name

ATP sensitive inward rectifier potassium channel 11 (KCNJ11), ELISA Kit

Full Product Name

Human ATP sensitive inward rectifier potassium channel 11 (KCNJ11) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
610582
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
50-1000pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of KCNJ11 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for KCNJ11purchase
MBS7224583 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ATP sensitive inward rectifier potassium channel 11 (KCNJ11) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KCNJ11. The ELISA analytical biochemical technique of the MBS7224583 kit is based on KCNJ11 antibody-KCNJ11 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KCNJ11 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KCNJ11. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Product Categories/Family for KCNJ11 elisa kit
NCBI/Uniprot data below describe general gene information for KCNJ11. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
33,263 Da
NCBI Official Full Name
ATP-sensitive inward rectifier potassium channel 11 isoform 1
NCBI Official Synonym Full Names
potassium inwardly-rectifying channel, subfamily J, member 11
NCBI Official Symbol
KCNJ11  [Similar Products]
NCBI Official Synonym Symbols
BIR; HHF2; PHHI; IKATP; TNDM3; KIR6.2
  [Similar Products]
NCBI Protein Information
ATP-sensitive inward rectifier potassium channel 11; beta-cell inward rectifier subunit; inward rectifier K(+) channel Kir6.2; inwardly rectifying potassium channel KIR6.2; potassium channel inwardly rectifing subfamily J member 11; potassium channel, inwardly rectifying subfamily J member 11
UniProt Protein Name
ATP-sensitive inward rectifier potassium channel 11
UniProt Synonym Protein Names
IKATP; Inward rectifier K(+) channel Kir6.2; Potassium channel, inwardly rectifying subfamily J member 11
UniProt Gene Name
KCNJ11  [Similar Products]
UniProt Entry Name
KCJ11_HUMAN
NCBI Summary for KCNJ11
Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and is found associated with the sulfonylurea receptor SUR. Mutations in this gene are a cause of familial persistent hyperinsulinemic hypoglycemia of infancy (PHHI), an autosomal recessive disorder characterized by unregulated insulin secretion. Defects in this gene may also contribute to autosomal dominant non-insulin-dependent diabetes mellitus type II (NIDDM), transient neonatal diabetes mellitus type 3 (TNDM3), and permanent neonatal diabetes mellitus (PNDM). Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Oct 2009]
UniProt Comments for KCNJ11
Kir6.2: This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium. Subunit of ATP-sensitive potassium channels (KATP). Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation. Defects in KCNJ11 are the cause of familial hyperinsulinemic hypoglycemia type 2 (HHF2); also known as persistent hyperinsulinemic hypoglycemia of infancy (PPHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. It causes nesidioblastosis, a diffuse abnormality of the pancreas in which there is extensive, often disorganized formation of new islets. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur. Defects in KCNJ11 are a cause of diabetes mellitus permanent neonatal (PNDM). PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy. Defects in KCNJ11 are the cause of transient neonatal diabetes mellitus type 3 (TNDM3). Neonatal diabetes mellitus, defined as insulin-requiring hyperglycemia within the first month of life, is a rare entity. In about half of the neonates, diabetes is transient and resolves at a median age of 3 months, whereas the rest have a permanent form of diabetes. In a significant number of patients with transient neonatal diabetes mellitus, diabetes type 2 appears later in life. The onset and severity of TNDM3 is variable with childhood-onset diabetes, gestational diabetes or adult-onset diabetes described. Defects in KCNJ11 may contribute to non-insulin- dependent diabetes mellitus (NIDDM), also known as diabetes mellitus type 2. Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ11 subfamily.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, potassium

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: voltage-gated potassium channel complex; mitochondrion; integral to plasma membrane; endoplasmic reticulum; T-tubule; ATP-sensitive potassium channel complex; nuclear envelope; axolemma; cytosol; cell soma; plasma membrane; endosome; myelin sheath

Molecular Function: potassium ion binding; protein C-terminus binding; voltage-gated potassium channel activity; G-protein activated inward rectifier potassium channel activity; heat shock protein binding; ATP-activated inward rectifier potassium channel activity; ankyrin binding; ATP binding

Biological Process: response to drug; synaptic transmission; regulation of membrane potential; potassium ion import; energy reserve metabolic process; glucose metabolic process; response to testosterone stimulus; regulation of insulin secretion; response to ATP; response to estradiol stimulus; neurological system process; negative regulation of insulin secretion

Disease: Maturity-onset Diabetes Of The Young, Type 13; Hyperinsulinemic Hypoglycemia, Familial, 2; Diabetes Mellitus, Transient Neonatal, 3; Diabetes Mellitus, Permanent Neonatal; Diabetes Mellitus, Noninsulin-dependent
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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