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anti-KDM1A antibody :: Rabbit KDM1A Antibody

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Catalog # MBS8502059
Unit / Price
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  0.1 mL  /  $310 +1 FREE 8GB USB
Western blot
Product Name

KDM1A, Antibody

Popular Item
Full Product Name

KDM1A Antibody

Product Synonym Names
KDM1; AOF2; BHC110; KIAA0601; LSD1; KDM1A
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
609132
3D Structure
ModBase 3D Structure for O60341
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity purification
Immunogen
Recombinant protein of human KDM1A
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-KDM1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-KDM1A antibody
Lysine-specific demethylase 1 (LSD1; also known as AOF2 and BHC110) is a nuclear amine oxidase homolog that acts as a histone demethylase and transcription cofactor (1). Gene activation and repression is specifically regulated by the methylation state of distinct histone protein lysine residues. For example, methylation of histone H3 at Lys4 facilitates transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes (2, 3). In contrast, methylation of histone H3 at Lys9 facilitates transcriptional repression by recruiting HP1 (4, 5). LSD1 is a component of the CoREST transcriptional co-repressor complex that also contains CoREST, CtBP, HDAC1 and HDAC2. As part of this complex, LSD1 demethylates mono-methyl and di-methyl histone H3 at Lys4 through a FAD-dependent oxidation reaction to facilitate neuronal-specific gene repression in non-neuronal cells (1, 6, 7). In contrast, LSD1 associates with androgen receptor in human prostate cells to demethylate mono-methyl and di-methyl histone H3 at Lys9 and facilitate androgen receptor-dependent transcriptional activation (8). Therefore, depending on gene context LSD1 can function as either a transcriptional co-repressor or co-activator. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine (8).
Applications Tested/Suitable for anti-KDM1A antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-KDM1A antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Western blot of anti-KDM1A antibody
Western blot analysis of extracts of PC12 celllysate using KDM1A antibody.
anti-KDM1A antibody Western blot image
NCBI/Uniprot data below describe general gene information for KDM1A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
95kDa
NCBI Official Full Name
lysine-specific histone demethylase 1A isoform a
NCBI Official Synonym Full Names
lysine demethylase 1A
NCBI Official Symbol
KDM1A  [Similar Products]
NCBI Official Synonym Symbols
AOF2; CPRF; KDM1; LSD1; BHC110
  [Similar Products]
NCBI Protein Information
lysine-specific histone demethylase 1A
UniProt Protein Name
Lysine-specific histone demethylase 1A
UniProt Synonym Protein Names
BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2
UniProt Gene Name
KDM1A  [Similar Products]
UniProt Synonym Gene Names
AOF2; KDM1; KIAA0601; LSD1  [Similar Products]
NCBI Summary for KDM1A
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
UniProt Comments for KDM1A
Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.
Product References and Citations for anti-KDM1A antibody
1. Shi, Y. et al. (2004) Cell 119, 941-953. 2. Wysocka, J. et al. (2006) Nature 442, 86-90. 3. Wysocka, J. et al. (2005) Cell 121, 859-872. 4. Jacobs, S.A. and Khorasanizadeh, S. (2002) Science 295, 2080-2083. 5. Nielsen, P.R. et al. (2002) Nature 416, 103-107. 6. Shi, Y.J. et al. (2005) Mol. Cell 19, 857-864. 7. Lee, M.G. et al. (2005) Nature 437, 432-435. 8. Metzger, E. et al. (2005) Nature 437, 436-439.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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