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KEAP1 elisa kit :: Chicken Kelch-like ECH-associated protein 1 (KEAP1) ELISA Kit

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Catalog # MBS7202152
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Kelch-like ECH-associated protein 1 (KEAP1), ELISA Kit

Popular Item
Also Known As

Chicken Kelch-like ECH-associated protein 1 (KEAP1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
606016
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of KEAP1. No significant cross-reactivity or interference between KEAP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between KEAP1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of KEAP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forKEAP1purchase
MBS7202152 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Kelch-like ECH-associated protein 1 (KEAP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KEAP1. The ELISA analytical biochemical technique of the MBS7202152 kit is based on KEAP1 antibody-KEAP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KEAP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KEAP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
KEAP1 elisa kit
Intended Uses: This KEAP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken KEAP1.

Principle of the Assay: KEAP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KEAP1 antibody and an KEAP1-HRP conjugate. The assay sample and buffer are incubated together with KEAP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KEAP1 concentration since KEAP1 from samples and KEAP1-HRP conjugate compete for the anti-KEAP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by KEAP1 from the sample, fewer sites are left to bind KEAP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KEAP1 concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of KEAP1 elisa kit
KEAP1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS7202152. Click to request current manual
NCBI/Uniprot data below describe general gene information for KEAP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
69,666 Da
NCBI Official Full Name
kelch-like ECH-associated protein 1
NCBI Official Synonym Full Names
kelch-like ECH-associated protein 1
NCBI Official Symbol
KEAP1  [Similar Products]
NCBI Official Synonym Symbols
INrf2; KLHL19
  [Similar Products]
NCBI Protein Information
kelch-like ECH-associated protein 1; kelch-like protein 19; cytosolic inhibitor of Nrf2; kelch-like family member 19
UniProt Protein Name
Kelch-like ECH-associated protein 1
UniProt Synonym Protein Names
Cytosolic inhibitor of Nrf2; INrf2; Kelch-like protein 19
UniProt Gene Name
KEAP1  [Similar Products]
UniProt Synonym Gene Names
INRF2; KIAA0132; KLHL19; INrf2  [Similar Products]
UniProt Entry Name
KEAP1_HUMAN
NCBI Summary for KEAP1
This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for KEAP1
KEAP1: a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Has tumor suppressor activity. Retains NRF2, BPTF and PGAM5 in the cytosol. Targets NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and of antioxidant response element-mediated detoxifying enzyme gene expression. Somatic mutations of KEAP1 or NRF2 commonly occur in solid cancers, irrespective of histological type, resulting in the constitutive transcription of cytoprotective genes. Genetic disruption of the KEAP1/CUL3 E3 ubiquitin ligase complex leads to the activation of the NF-kappaB pathway in lung cancer. Alternative activation of the cytoprotective NRF2 pathway exist in the absence of mutations in KEAP1 and HRF2: WTX, PALB2, SQSTM1, and DPP3 can competitively bind KEAP1. These proteins and others that bind KEAP1 contain an ¿ETGE¿ amino acid motif, which matches the KEAP1 interaction motif found in NRF2. Binding of these ¿ETGE¿-containing proteins can displace NRF2, freeing it to translocate to the nucleus and implement its cytoprotective transcriptional pathway. The DPP3 gene is frequently overexpressed in squamous cell lung carcinomas with high wt NRF2 activity. Its expression level significantly correlates with the response rate and progression-free survival of platinum-based first-line chemotherapy. May be a useful biomarker for predicting the chemosensitivity of patients with advanced-stage NSCLC. Broadly expressed, with highest levels in skeletal muscle. Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane.

Protein type: Ubiquitin conjugating system; Endoplasmic reticulum; Transcription regulation; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; endoplasmic reticulum; cytoplasm; microtubule organizing center; midbody; cytosol

Molecular Function: protein binding; transcription factor binding

Biological Process: transcription, DNA-dependent; in utero embryonic development; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of transcription factor activity; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; regulation of epidermal cell differentiation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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