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KHSRP elisa kit :: Human KH-type splicing regulatory protein ELISA Kit

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Catalog # MBS924450
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Typical Testing Data/Standard Curve (for reference only)
Product Name

KH-type splicing regulatory protein (KHSRP), ELISA Kit

Popular Item
Full Product Name

Human Far upstream element-binding protein 2, KHSRP ELISA Kit

Product Synonym Names
Human Far upstream element-binding protein 2 (KHSRP) ELISA kit; FBP2; FUBP2; KSRP; MGC99676; FUSE binding protein 2; far upstream element-binding protein 2; KH-type splicing regulatory protein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS924450 Testing Data
OMIM
603445
3D Structure
ModBase 3D Structure for Q92945
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human KHSRP. No significant cross-reactivity or interference between human KHSRP and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates
Assay Type
Quantitative Sandwich
Detection Range
39 pg/ml -2500 pg/ml
Sensitivity
9.75 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of KHSRP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forKHSRPpurchase
MBS924450 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the KH-type splicing regulatory protein (KHSRP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KHSRP. The ELISA analytical biochemical technique of the MBS924450 kit is based on KHSRP antibody-KHSRP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KHSRP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KHSRP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
KHSRP elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KHSRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KHSRP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KHSRP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KHSRP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of KHSRP elisa kit
KHSRP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS924450. Click to request current manual
NCBI/Uniprot data below describe general gene information for KHSRP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
73,115 Da
NCBI Official Full Name
far upstream element-binding protein 2
NCBI Official Synonym Full Names
KH-type splicing regulatory protein
NCBI Official Symbol
KHSRP  [Similar Products]
NCBI Official Synonym Symbols
FBP2; KSRP; FUBP2
  [Similar Products]
NCBI Protein Information
far upstream element-binding protein 2; p75; FUSE binding protein 2; FUSE-binding protein 2; KH type-splicing regulatory protein
UniProt Protein Name
Far upstream element-binding protein 2
UniProt Synonym Protein Names
KH type-splicing regulatory protein; KSRP; p75
UniProt Gene Name
KHSRP  [Similar Products]
UniProt Synonym Gene Names
FUBP2; FUSE-binding protein 2; KSRP  [Similar Products]
UniProt Entry Name
FUBP2_HUMAN
NCBI Summary for KHSRP
The KHSRP gene encodes a multifunctional RNA-binding protein implicated in a variety of cellular processes, including transcription, alternative pre-mRNA splicing, and mRNA localization (Min et al., 1997 [PubMed 9136930]; Gherzi et al., 2004 [PubMed 15175153]).[supplied by OMIM, Apr 2010]
UniProt Comments for KHSRP
KHSRP: an RNA binding and processing protein that binds to the dendritic targeting element and may play a role in mRNA trafficking. Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3' untranslated regions, possibly by recruiting degradation machinery to ARE-containing mRNAs. Interacts with APC which is part of the apolipoprotein B mRNA editing enzyme complex. The interaction is not required for activity of the catalytic component, APOBEC1, but may confer stability on the complex.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; membrane; stress granule; cytosol

Molecular Function: mRNA binding; DNA binding

Biological Process: RNA splicing, via transesterification reactions; mRNA transport; transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA splicing; mRNA catabolic process; gene expression; mRNA processing
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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