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Ki67P elisa kit :: Human Ki-67 Protein (Ki67P) ELISA Kit

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Catalog # MBS2704118 (SPECIAL PROMOTIONAL PRICING for a limited time)
Unit / Price
  24-Strip-Wells  /  $220 +1 FREE 8GB USB
  48-Strip-Wells  /  $355 +1 FREE 8GB USB
  96-Strip-Wells  /  $475 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,975 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,675 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Ki-67 Protein (Ki67P), ELISA Kit

Popular Item
Also Known As

Human Ki-67 Protein (Ki67P) ELISA Kit

Product Synonym Names
MKI67; KIA; Ki-67; Antigen Identified By Monoclonal Antibody Ki-67
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
2,936
3D Structure
ModBase 3D Structure for Q61769
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Ki-67 Protein (Ki67P).
No significant cross-reactivity or interference between Ki-67 Protein (Ki67P) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
Detection Range
0.78-50ng/mL
Sensitivity
< 0.32ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Ki-67 Protein (Ki67P) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Ki-67 Protein (Ki67P) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Ki67P elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forKi67Ppurchase
MBS2704118 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ki-67 Protein (Ki67P) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Ki67P. The ELISA analytical biochemical technique of the MBS2704118 kit is based on Ki67P antibody-Ki67P antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Ki67P antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Ki67P. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Ki67P elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ki-67 Protein (Ki67P). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ki-67 Protein (Ki67P). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ki-67 Protein (Ki67P), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ki-67 Protein (Ki67P) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of Ki67P elisa kit
Ki67P elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2704118. Click to request current manual
NCBI/Uniprot data below describe general gene information for Ki67P. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
324,718 Da
NCBI Official Full Name
Ki-67
NCBI Official Synonym Full Names
antigen identified by monoclonal antibody Ki 67
NCBI Official Symbol
Mki67  [Similar Products]
NCBI Official Synonym Symbols
Ki67; Ki-67; D630048A14Rik
  [Similar Products]
NCBI Protein Information
proliferation marker protein Ki-67; antigen KI-67
UniProt Protein Name
Proliferation marker protein Ki-67
UniProt Synonym Protein Names
Antigen identified by monoclonal antibody Ki-67 homologCurated; Antigen KI-67 homologCurated; Antigen Ki67 homologCurated
UniProt Gene Name
Mki67  [Similar Products]
UniProt Synonym Gene Names
Antigen KI-67 homologCurated; Antigen Ki67 homologCurated  [Similar Products]
UniProt Comments for Ki67P
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (). Does not contribute to the internal structure of mitotic chromosomes (PubMed:26949251). May play a role in chromatin organization (PubMed:26949251). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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