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KIAA1199 elisa kit :: Human KIAA1199 ELISA Kit

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Catalog # MBS701499
Unit / Price
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Typical Testing Data/Standard Curve (for reference only)
Product Name

KIAA1199, ELISA Kit

Popular Item
Full Product Name

Human KIAA1199 ELISA Kit

Product Synonym Names
Human KIAA1199 ELISA Kit; TMEM2L; transmembrane protein 2-like; KIAA1199
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS701499 Testing Data
OMIM
608366
3D Structure
ModBase 3D Structure for Q8WUJ3
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human KIAA 1199. No significant cross-reactivity or interference between human KIAA 1199 and analogues was observed.
Samples
serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
1 ng/ml -24 ng/ml
Sensitivity
<0.4 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<10%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of KIAA1199 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for KIAA1199purchase
MBS701499 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the KIAA1199, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KIAA1199. The ELISA analytical biochemical technique of the MBS701499 kit is based on KIAA1199 antibody-KIAA1199 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KIAA1199 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KIAA1199. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
KIAA1199 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KIAA1199 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for KIAA1199. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIAA1199 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of KIAA1199 elisa kit
KIAA1199 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS701499. Click to request current manual
NCBI/Uniprot data below describe general gene information for KIAA1199. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
152,998 Da
NCBI Official Full Name
cell migration-inducing and hyaluronan-binding protein
NCBI Official Synonym Full Names
cell migration inducing protein, hyaluronan binding
NCBI Official Symbol
CEMIP  [Similar Products]
NCBI Official Synonym Symbols
CCSP1; TMEM2L; KIAA1199
  [Similar Products]
NCBI Protein Information
cell migration-inducing and hyaluronan-binding protein; colon cancer secreted protein 1
UniProt Protein Name
Cell migration-inducing and hyaluronan-binding protein
UniProt Gene Name
CEMIP  [Similar Products]
UniProt Synonym Gene Names
KIAA1199  [Similar Products]
UniProt Entry Name
CEMIP_HUMAN
UniProt Comments for KIAA1199
Function: Mediates depolymerization of hyaluronic acid (HA) via the cell membrane-associated clathrin-coated pit endocytic pathway. Binds to hyaluronic acid. Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product, a process that may occur through rapid vesicle endocytosis and recycling without intracytoplasmic accumulation or digestion in lysosomes. Involved in hyaluronan catabolism in the dermis of the skin and arthritic synovium. Positively regulates epithelial-mesenchymal transition (EMT), and hence tumor cell growth, invasion and cancer dissemination. In collaboration with HSPA5/BIP, promotes cancer cell migration in a calcium and PKC-dependent manner. May be involved in hearing.4 PublicationsManual assertion based on experiment in:Ref.10

Catalytic activity: Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.2 PublicationsManual assertion based on experiment in:Ref.14

Enzyme regulation: Activity is up-regulated by histamine.1 PublicationManual assertion based on experiment in:Ref.14
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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