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KIAA1524 elisa kit :: Human cancerous inhibitor of PP2A (CIP2A) ELISA Kit

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Catalog # MBS7205249
Unit / Price
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  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

cancerous inhibitor of PP2A (CIP2A) (KIAA1524), ELISA Kit

Popular Item
Full Product Name

Human cancerous inhibitor of PP2A (CIP2A) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS7205249 COA
Species Reactivity
This assay has high sensitivity and excellent specificity for detection of CIP2A. No significant cross-reactivity or interference between CIP2A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CIP2A and all the analogues, therefore, cross reaction may still exist in some cases.
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Detection Range
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of KIAA1524 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for KIAA1524purchase
MBS7205249 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cancerous inhibitor of PP2A (CIP2A) (KIAA1524) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KIAA1524. The ELISA analytical biochemical technique of the MBS7205249 kit is based on KIAA1524 antibody-KIAA1524 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KIAA1524 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KIAA1524. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
KIAA1524 elisa kit
Intended Uses: This CIP2A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CIP2A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: CIP2A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CIP2A antibody and an CIP2A-HRP conjugate. The assay sample and buffer are incubated together with CIP2A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CIP2A concentration since CIP2A from samples and CIP2A-HRP conjugate compete for the anti-CIP2A antibody binding site. Since the number of sites is limited, as more sites are occupied by CIP2A from the sample, fewer sites are left to bind CIP2A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CIP2A concentration in each sample is interpolated from this standard curve.
Product Categories/Family for KIAA1524 elisa kit

Typical Standard Curve/Testing Data of KIAA1524 elisa kit
KIAA1524 elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS7205249. Click to request current manual
NCBI/Uniprot data below describe general gene information for KIAA1524. It may not necessarily be applicable to this product.
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
84,579 Da
NCBI Official Full Name
NCBI Official Synonym Full Names
NCBI Official Symbol
KIAA1524  [Similar Products]
NCBI Official Synonym Symbols
p90; CIP2A
  [Similar Products]
NCBI Protein Information
protein CIP2A; p90 autoantigen; cancerous inhibitor of PP2A; cancerous inhibitor of protein phosphatase 2A
UniProt Protein Name
Protein CIP2A
UniProt Synonym Protein Names
Cancerous inhibitor of PP2A; p90 autoantigen
Protein Family
UniProt Gene Name
KIAA1524  [Similar Products]
UniProt Synonym Gene Names
CIP2A  [Similar Products]
UniProt Entry Name
UniProt Comments for KIAA1524
CIP2A: an oncoprotein that inhibits PP2A in human malignancies. Like the SV40 small t antigen, inhibits the phospho-serine/-threonine phosphatase PP2A. Promotes c-Myc protein stability by inhibiting the dephosphorylation of phospho-serine 62. Expressed at low levels in most of nonmalignant tissues but at high levels in human malignancies including head and neck squamous cell carcinoma (HNSCC), colon cancer, and liver cancer. Antibodies against CIP2A are present in sera from many patients with gastric or prostate cancer. Two alternatively spliced human isoforms have been described.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q13.13

Cellular Component: cytoplasm; integral to membrane; plasma membrane

Molecular Function: protein binding

Biological Process: spermatogenesis
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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