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Kim-1 elisa kit :: Human Kim-1 ELISA Kit

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Catalog # MBS355384
Unit / Price
  96 Tests  /  $440 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Kim-1, ELISA Kit

Popular Item
Also Known As

Kim-1 Human ELISA Kit

Product Synonym Names
Human Kim-1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
Samples
Human serum, plasma, urine, cell culture supernatant or tissue samples.
Assay Type
Sandwich
Detection Range
31.2 pg/ml-2000 pg/ml
Preparation and Storage
Store at 2-8 degree C for 6 months.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Kim-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forKim-1purchase
MBS355384 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Kim-1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Kim-1. The ELISA analytical biochemical technique of the MBS355384 kit is based on Kim-1 antibody-Kim-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Kim-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Kim-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Kim-1 elisa kit
Principle of the Assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-KIM-1 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-KIM-1 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the KIM-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of KIM-1 can be calculated.

Background: KIM1, also known as Hepatitis A virus cellular receptor 1, is a protein that in humans is encoded by the HAVCR1 gene, which maps to 5q33.2. It is a major cause of orally transmitted acute hepatitis, infects primate cells, but not dog or rat cells, after binding to the HAV cellular receptor (HAVCR). Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Khademi et al. (2004) found that differential expression of TIMs by Th1 and Th2 cells may be implicated in different phases of an autoimmune disease.
Applications Tested/Suitable for Kim-1 elisa kit
Quantitative sELISA (EIA)
Application Notes for Kim-1 elisa kit
For quantitative detection of KIM-1 in human serum, plasma, urine, cell culture supernatant or tissue samples.

Typical Testing Data/Standard Curve (for reference only) of Kim-1 elisa kit
Kim-1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS355384. Click to request current manual
NCBI/Uniprot data below describe general gene information for Kim-1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
NCBI Official Full Name
kidney injury molecule-1
UniProt Comments for Kim-1
May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair ().
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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