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KISS1 elisa kit :: Human Kisspeptin 1 (KISS1) ELISA Kit

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Catalog # MBS454153
Unit / Price
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  48-Strip-Wells  /  $400 +1 FREE 8GB USB
  96-Strip-Wells  /  $535 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,425 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,710 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Kisspeptin 1 (KISS1), ELISA Kit

Popular Item
Full Product Name

Human Kisspeptin 1 (KISS1) ELISA Kit

Product Synonym Names
Metastin; KiSS-1 Metastasis-Suppressor
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
U43527 mRNA
3D Structure
ModBase 3D Structure for Q15726
Species Reactivity
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Type
Sandwich
Detection Range
31.25-2000pg/mL
Sensitivity
12.9pg/mL
Intra Assay
CV<10%
Inter Assay
CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of KISS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for KISS1purchase
MBS454153 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Kisspeptin 1 (KISS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KISS1. The ELISA analytical biochemical technique of the MBS454153 kit is based on KISS1 antibody-KISS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KISS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KISS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Typical Testing Data/Standard Curve (for reference only) of KISS1 elisa kit
KISS1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS454153. Click to request current manual
NCBI/Uniprot data below describe general gene information for KISS1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
14,705 Da
NCBI Official Full Name
metastasis-suppressor KiSS-1 preproprotein
NCBI Official Synonym Full Names
KiSS-1 metastasis-suppressor
NCBI Official Symbol
KISS1  [Similar Products]
NCBI Official Synonym Symbols
HH13; KiSS-1
  [Similar Products]
NCBI Protein Information
metastasis-suppressor KiSS-1
UniProt Protein Name
Metastasis-suppressor KiSS-1
UniProt Synonym Protein Names
Kisspeptin-1
Protein Family
UniProt Gene Name
KISS1  [Similar Products]
NCBI Summary for KISS1
This gene is a metastasis suppressor gene that suppresses metastases of melanomas and breast carcinomas without affecting tumorigenicity. The encoded protein may inhibit chemotaxis and invasion and thereby attenuate metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A protein product of this gene, kisspeptin, stimulates gonadotropin-releasing hormone (GnRH)-induced gonadotropin secretion and regulates the pubertal activation of GnRH nuerons. A polymorphism in the terminal exon of this mRNA results in two protein isoforms. An adenosine present at the polymorphic site represents the third position in a stop codon. When the adenosine is absent, a downstream stop codon is utilized and the encoded protein extends for an additional seven amino acid residues. [provided by RefSeq, Mar 2012]
UniProt Comments for KISS1
Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca2+ levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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