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KISS1 elisa kit :: Mouse KiSS-1 metastasis-suppressor ELISA Kit

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Catalog # MBS916129
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

KiSS-1 metastasis-suppressor (KISS1), ELISA Kit

Popular Item
Also Known As

Mouse Metastasis-suppressor KiSS-1, KISS1 ELISA Kit

Product Synonym Names
Mouse Metastasis-suppressor KiSS-1 (KISS1) ELISA kit; KiSS-1; METASTIN; MGC39258; kisspeptin; malignant melanoma metastasis-suppressor; KiSS-1 metastasis-suppressor
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS916129 Testing Data
Sequence Length
3D Structure
ModBase 3D Structure for Q6Y4S4
Species Reactivity
This assay has high sensitivity and excellent specificity for detection of mouse KISS1. No significant cross-reactivity or interference between mouse KISS1 and analogues was observed.
Serum, plasma, cell culture supernates, tissue homogenates
Assay Type
Detection Range
12.5 pg/ml-800 pg/ml.
The minimum detectable dose of mouse KISS1 is typically less than 3.125 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of KISS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forKISS1purchase
MBS916129 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the KiSS-1 metastasis-suppressor (KISS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KISS1. The ELISA analytical biochemical technique of the MBS916129 kit is based on KISS1 antibody-KISS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KISS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KISS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
KISS1 elisa kit
Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KISS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KISS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KISS1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KISS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of KISS1 elisa kit
KISS1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS916129. Click to request current manual
NCBI/Uniprot data below describe general gene information for KISS1. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
14,117 Da
NCBI Official Full Name
metastasis-suppressor KiSS-1
NCBI Official Synonym Full Names
KiSS-1 metastasis-suppressor
NCBI Official Symbol
Kiss1  [Similar Products]
NCBI Official Synonym Symbols
  [Similar Products]
NCBI Protein Information
metastasis-suppressor KiSS-1; metastin; kisspeptin-1; 0610012C01Rik; Kiss1 metastasis-suppressor
UniProt Protein Name
Metastasis-suppressor KiSS-1
UniProt Synonym Protein Names
Kisspeptin-1Cleaved into the following 2 chains:MetastinAlternative name(s):Kisspeptin-52
Protein Family
UniProt Gene Name
Kiss1  [Similar Products]
UniProt Entry Name
UniProt Comments for KISS1
KiSS-1: Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell- matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine- tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Belongs to the KISS1 family.

Protein type: Cell adhesion; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; neuron projection; cell soma; apical plasma membrane; cytoplasm; cytosol

Molecular Function: protein binding; G-protein-coupled receptor binding; kisspeptin receptor binding

Biological Process: positive regulation of growth hormone secretion; generation of ovulation cycle rhythm; negative regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); elevation of cytosolic calcium ion concentration; positive regulation of MAPKKK cascade; negative regulation of cell migration; positive regulation of synaptic transmission; positive regulation of luteinizing hormone secretion
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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