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KITLG recombinant protein :: STEM CELL FACTOR Recombinant Protein

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Catalog # MBS232270
Unit / Price
  0.01 mg  /  $440 +1 FREE 8GB USB
KITLG recombinant protein
Product Name

STEM CELL FACTOR (KITLG), Recombinant Protein

Also Known As

RECOMBINANT HUMAN STEM CELL FACTOR

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
273
OMIM
145250
3D Structure
ModBase 3D Structure for P21583
Purity/Purification
>98% by SDS PAGE and HPLC analysis
Form/Format
Purified recombinant protein expressed in E Coli - lyophilised
Concentration
Total protein concentration 0.1mg/ml after reconstitution (lot specific)
Reconstitution
Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
Buffer Solution
10mM Acetic acid
Activity
5 x 105 U/mg as determined by dose-dependent stimulation of proliferation on human TF-1 cells.
Target Species
Human
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution
Other Notes
Small volumes of KITLG recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
KITLG recombinant protein
Stem cell factor (SCF) is a haematopoietic growth factor acting during early stages of haemopoiesis. SCF stimulates proliferation of myeloid, erythroid and lymphoid progenitors in bone marrow cultures and appears to act synergistically with the colony stimulating factors.
Applications Tested/Suitable for KITLG recombinant protein
ELISA (EIA), Functional Assays (FN), Western Blot (WB)
Application Notes for KITLG recombinant protein
ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well;
Functional Assays: Minimum Dilution: 0.2; Maximum Dilution: 20.0ng/ml;
Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
NCBI/Uniprot data below describe general gene information for KITLG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Protein Molecular Weight: 18.4kD (165 Amino acid sequence)[Similar Products]
NCBI Official Full Name
kit ligand isoform b
NCBI Official Synonym Full Names
KIT ligand
NCBI Official Symbol
KITLG  [Similar Products]
NCBI Official Synonym Symbols
SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7
  [Similar Products]
NCBI Protein Information
kit ligand; c-Kit ligand; familial progressive hyperpigmentation 2; mast cell growth factor; steel factor; stem cell factor
UniProt Protein Name
Kit ligand
UniProt Synonym Protein Names
Mast cell growth factor; MGF; Stem cell factor; SCF; c-Kit ligandSoluble KIT ligand; sKITLG
Protein Family
UniProt Gene Name
KITLG  [Similar Products]
UniProt Synonym Gene Names
MGF; SCF; MGF; SCF; sKITLG  [Similar Products]
UniProt Entry Name
SCF_HUMAN
NCBI Summary for KITLG
This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for KITLG
SCF: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Belongs to the SCF family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: extracellular space; cytoskeleton; cytoplasm; extracellular region; integral to membrane; plasma membrane

Molecular Function: protein binding; growth factor activity; cytokine activity; stem cell factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; embryonic hemopoiesis; germ cell programmed cell death; male gonad development; signal transduction; positive regulation of MAP kinase activity; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of melanocyte differentiation; negative regulation of mast cell apoptosis; innate immune response; positive regulation of myeloid leukocyte differentiation; positive regulation of Ras protein signal transduction; cell adhesion; neural crest cell migration; positive regulation of DNA replication

Disease: Hyperpigmentation, Familial Progressive, 2; Skin/hair/eye Pigmentation, Variation In, 7
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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