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KL elisa kit :: Human Klotho ELISA Kit

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Catalog # MBS2886510
Unit / Price
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  48-Strip-Wells  /  $355 +1 FREE 8GB USB
  96-Strip-Wells  /  $475 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,030 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,700 +4 FREE 8GB USB
Typical Testing Data/Standard Curve
Product Name

Klotho, ELISA Kit

Popular Item
Full Product Name

Human Klotho ELISA Kit

Product Synonym Names
Klotho; KL; 3.2.1.31
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2886510 Testing Data
OMIM
211900
3D Structure
ModBase 3D Structure for Q9UEF7
Species Reactivity
Assay Type
Sandwich
Detection Range
7.8-500 pg/mL
Sensitivity
2.4 pg/mL
Intra-Assay CV
<=4.7%
Inter-Assay CV
<=7.1%
Recovery
104%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of KL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for KLpurchase
MBS2886510 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Klotho, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KL. The ELISA analytical biochemical technique of the MBS2886510 kit is based on KL antibody-KL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
KL elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve of KL elisa kit
KL elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS2886510. Click to request current manual
NCBI/Uniprot data below describe general gene information for KL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
62,135 Da
NCBI Official Full Name
klotho
NCBI Official Synonym Full Names
klotho
NCBI Official Symbol
NCBI Protein Information
klotho
UniProt Protein Name
Klotho
Protein Family
UniProt Gene Name
UniProt Entry Name
KLOT_HUMAN
NCBI Summary for KL
This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008]
UniProt Comments for KL
Klotho: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.2.1.31; Membrane protein, integral; Hydrolase

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: apical plasma membrane; extracellular region; extracellular space; integral to membrane; integral to plasma membrane; intracellular; plasma membrane

Molecular Function: beta-glucosidase activity; beta-glucuronidase activity; fibroblast growth factor binding; fibroblast growth factor receptor binding; hormone activity; signal transducer activity; vitamin D binding

Biological Process: activation of MAPKK activity; aging; axon guidance; calcium ion homeostasis; carbohydrate metabolic process; energy reserve metabolic process; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of bone mineralization; Ras protein signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway

Disease: Tumoral Calcinosis, Hyperphosphatemic, Familial
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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