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KL elisa kit :: Mouse klotho ELISA Kit

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Catalog # MBS702609
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

klotho, ELISA Kit

Popular Item
Full Product Name

Mouse Klotho ELISA Kit

Product Synonym Names
Mouse Klotho ELISA Kit; klotho
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS702609 Testing Data
3D Structure
ModBase 3D Structure for O35082
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse klotho. No significant cross-reactivity or interference between mouse klotho and analogues was observed.
Samples
Serum, plasma, cell culture supernates
Detection Range
3.12 pg/ml -200 pg/mL
Sensitivity
<0.78 pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of KL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forKLpurchase
MBS702609 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the klotho, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KL. The ELISA analytical biochemical technique of the MBS702609 kit is based on KL antibody-KL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
KL elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for klotho has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any klotho present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for klotho is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of klotho bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of KL elisa kit
KL elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS702609. Click to request current manual
NCBI/Uniprot data below describe general gene information for KL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
116,398 Da
NCBI Official Full Name
klotho
NCBI Official Synonym Full Names
klotho
NCBI Official Symbol
NCBI Official Synonym Symbols
alpha-kl
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NCBI Protein Information
klotho; secreted form of Klotho protein
UniProt Protein Name
Klotho
Protein Family
UniProt Gene Name
UniProt Entry Name
KLOT_MOUSE
UniProt Comments for KL
Function: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1.6 PublicationsManual assertion based on experiment in:Ref.1

Catalytic activity: A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.2 PublicationsManual assertion based on experiment in:Ref.13

Enzyme regulation: Inhibited by D-saccharic acid 1,4-lactone and taurocholic acid.1 PublicationManual assertion based on experiment in:Ref.13
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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