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KL recombinant protein :: Klotho (KL) Recombinant Protein

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Catalog # MBS2010008 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $135 +1 FREE 8GB USB
  0.05 mg  /  $245 +1 FREE 8GB USB
  0.1 mg  /  $365 +1 FREE 8GB USB
  0.2 mg  /  $450 +1 FREE 8GB USB
  0.5 mg  /  $870 +1 FREE 8GB USB
  1 mg  /  $1,280 +1 FREE 8GB USB
Product Name

Klotho (KL), Recombinant Protein

Popular Item
Also Known As

Recombinant Klotho (KL)

Matching Pairs
Unconjugated Antibody: Klotho (KL) (MBS2003676)
Immunogen: Klotho (KL) (MBS2010008)
Matching Pairs
APC-CY7 Conjugated Antibody: Klotho (KL) (MBS2080284)
Immunogen: Klotho (KL) (MBS2010008)
Matching Pairs
PE Conjugated Antibody: Klotho (KL) (MBS2080286)
Immunogen: Klotho (KL) (MBS2010008)
Matching Pairs
APC Conjugated Antibody: Klotho (KL) (MBS2080288)
Immunogen: Klotho (KL) (MBS2010008)
Matching Pairs
Cy3 Conjugated Antibody: Klotho (KL) (MBS2080290)
Immunogen: Klotho (KL) (MBS2010008)
Matching Pairs
FITC Conjugated Antibody: Klotho (KL) (MBS2080291)
Immunogen: Klotho (KL) (MBS2010008)
Matching Pairs
HRP Conjugated Antibody: Klotho (KL) (MBS2080293)
Immunogen: Klotho (KL) (MBS2010008)
Matching Pairs
Biotin Conjugated Antibody: Klotho (KL) (MBS2096487)
Immunogen: Klotho (KL) (MBS2010008)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS2010008 Technical Datasheet
Chromosome Location
Chromosome: 5; NC_000071.6 (150952607..150993817). Location: 5; 5 G3
3D Structure
ModBase 3D Structure for O35082
Host
E Coli
Purity/Purification
> 90%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Concentration
Original Concentration: 200 Ug/mL (lot specific)
Organism Species
Mus musculus(Mouse)
Source
Prokaryotic expression
Residues
Leu59~Phe508
Tags
N-terminal His Tag
Subcellular Location
Membrane, Secreted
Traits
Freeze-dried powder
Buffer formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Predicted isoelectric point
8.3
Predicted Molecular Mass
52.7kDa
Accurate Molecular Mass
53kDa as determined by SDS-PAGE reducing conditions
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Other Notes
Small volumes of KL recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for KL recombinant protein
Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Sequence Information of KL recombinant protein
KL recombinant protein Sequence Information image
NCBI/Uniprot data below describe general gene information for KL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
klotho
NCBI Official Synonym Full Names
klotho
NCBI Official Symbol
NCBI Official Synonym Symbols
alpha-kl
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NCBI Protein Information
klotho; secreted form of Klotho protein
UniProt Protein Name
Klotho
Protein Family
UniProt Gene Name
UniProt Entry Name
KLOT_MOUSE
UniProt Comments for KL
Klotho: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Hydrolase; EC 3.2.1.31

Cellular Component: extracellular space; membrane; extracellular region; plasma membrane; integral to membrane

Molecular Function: signal transducer activity; protein binding; beta-glucuronidase activity; fibroblast growth factor binding; hydrolase activity; hydrolase activity, acting on glycosyl bonds; hydrolase activity, hydrolyzing O-glycosyl compounds; fibroblast growth factor receptor binding

Biological Process: calcium ion homeostasis; fibroblast growth factor receptor signaling pathway; metabolic process; carbohydrate metabolic process; energy reserve metabolic process; insulin receptor signaling pathway; positive regulation of bone mineralization; acute inflammatory response; aging
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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