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KLK4 elisa kit :: Human kallikrein-related peptidase 4 ELISA Kit

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Catalog # MBS917102
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
KLK4 elisa kit
Product Name

kallikrein-related peptidase 4 (KLK4), ELISA Kit

Popular Item
Also Known As

Human Kallikrein-4, KLK4 ELISA Kit

Product Synonym Names
Human Kallikrein-4 (KLK4) ELISA kit; AI2A1; ARM1; EMSP; EMSP1; KLK-L1; MGC116827; MGC116828; PROSTASE; PRSS17; PSTS; androgen-regulated message 1; enamel matrix serine protease 1; kallikrein 4 (prostase; enamel matrix; prostate) ; kallikre; kallikrein-related peptidase 4
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
254
OMIM
603767
3D Structure
ModBase 3D Structure for Q9Y5K2
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human KLK4. No significant cross-reactivity or interference between human KLK4 and analogues was observed.
Samples
Serum, plasma, tissue homogenates.
Assay Type
Sandwich
Detection Range
0.156 ng/ml -10 ng/ml.
Sensitivity
The minimum detectable dose of human KLK4 is typically less than 0.039 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of KLK4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forKLK4purchase
MBS917102 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the kallikrein-related peptidase 4 (KLK4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KLK4. The ELISA analytical biochemical technique of the MBS917102 kit is based on KLK4 antibody-KLK4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KLK4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KLK4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
KLK4 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KLK4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLK4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS917102. Click to request current manual
NCBI/Uniprot data below describe general gene information for KLK4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
27,032 Da
NCBI Official Full Name
kallikrein-4 preproprotein
NCBI Official Synonym Full Names
kallikrein-related peptidase 4
NCBI Official Symbol
NCBI Official Synonym Symbols
ARM1; EMSP; PSTS; AI2A1; EMSP1; KLK-L1; PRSS17; kallikrein
  [Similar Products]
NCBI Protein Information
kallikrein-4; prostase; serine protease 17; kallikrein-like protein 1; androgen-regulated message 1; enamel matrix serine protease 1; enamel matrix serine proteinase 1
UniProt Protein Name
Kallikrein-4
UniProt Synonym Protein Names
Enamel matrix serine proteinase 1; Kallikrein-like protein 1; KLK-L1; Prostase; Serine protease 17
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
EMSP1; PRSS17; PSTS; KLK-L1  [Similar Products]
UniProt Entry Name
KLK4_HUMAN
NCBI Summary for KLK4
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. In some tissues its expression is hormonally regulated. The expression pattern of a similar mouse protein in murine developing teeth supports a role for the protein in the degradation of enamel proteins. Alternate splice variants for this gene have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for KLK4
KLK4: Involved in enamel formation. Defects in KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1). AI2A1 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: Protease; Secreted, signal peptide; EC 3.4.21.-; Secreted

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular region

Molecular Function: serine-type peptidase activity; metal ion binding; serine-type endopeptidase activity

Biological Process: extracellular matrix disassembly; protein catabolic process; proteolysis

Disease: Amelogenesis Imperfecta, Hypomaturation Type, Iia1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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