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KPNB1 elisa kit :: Human karyopherin (importin) beta 1 ELISA Kit

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Catalog # MBS9319158
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
KPNB1 elisa kit
Product Name

karyopherin (importin) beta 1 (KPNB1), ELISA Kit

Full Product Name

Human Importin subunit beta-1, KPNB1 ELISA Kit

Product Synonym Names
Human Importin subunit beta-1 (KPNB1) ELISA kit; IMB1; IPO1; IPOB; Impnb; MGC2155; MGC2156; MGC2157; NTF97; importin 1; importin 90; importin beta-1 subunit; karyopherin beta 1; nuclear factor p97; karyopherin (importin) beta 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
602738
3D Structure
ModBase 3D Structure for Q14974
Species Reactivity
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of KPNB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forKPNB1purchase
MBS9319158 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the karyopherin (importin) beta 1 (KPNB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KPNB1. The ELISA analytical biochemical technique of the MBS9319158 kit is based on KPNB1 antibody-KPNB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KPNB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KPNB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
NCBI/Uniprot data below describe general gene information for KPNB1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
97,170 Da
NCBI Official Full Name
importin subunit beta-1 isoform 2
NCBI Official Synonym Full Names
karyopherin (importin) beta 1
NCBI Official Symbol
KPNB1  [Similar Products]
NCBI Official Synonym Symbols
IMB1; IPO1; IPOB; Impnb; NTF97
  [Similar Products]
NCBI Protein Information
importin subunit beta-1; PTAC97; importin 1; importin 90; importin-90; nuclear factor p97; importin beta-1 subunit; karyopherin subunit beta-1; pore targeting complex 97 kDa subunit
UniProt Protein Name
Importin subunit beta-1
UniProt Synonym Protein Names
Importin-90; Karyopherin subunit beta-1; Nuclear factor p97; Pore targeting complex 97 kDa subunit; PTAC97
Protein Family
UniProt Gene Name
KPNB1  [Similar Products]
UniProt Synonym Gene Names
NTF97; PTAC97  [Similar Products]
UniProt Entry Name
IMB1_HUMAN
NCBI Summary for KPNB1
Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]
UniProt Comments for KPNB1
KPNB1: a nuclear import protein nuclear protein import that functions either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re- exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus. Forms a complex with an importin alpha subunit. Forms a heterodimer with IPO7. Interacts with IPO7, SNUPN, RPL23A and XPO1. Two isoforms of the human protein have been reported. Belongs to the importin beta family.

Protein type: Nuclear import; Karyopherin

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: nucleoplasm; nuclear membrane; membrane; cytoplasm; nuclear envelope; nuclear pore; cytosol

Molecular Function: protein domain specific binding; protein binding; enzyme binding; zinc ion binding; nuclear localization sequence binding; Ran GTPase binding; protein transporter activity

Biological Process: ribosomal protein import into nucleus; viral reproduction; apoptosis; protein import into nucleus; protein import into nucleus, translocation; cytokine and chemokine mediated signaling pathway; viral infectious cycle; NLS-bearing substrate import into nucleus; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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