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KRAS elisa kit :: Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog ELISA Kit

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Catalog # MBS936810
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Typical Testing Data/Standard Curve (for reference only)
Product Name

v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), ELISA Kit

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Full Product Name

Human GTPase KRas, KRAS ELISA Kit

Product Synonym Names
Human GTPase KRas (KRAS) ELISA kit; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RASK2; K-ras p21 protein; Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog; PR310 c-K-ras oncogene; c-K-ras2 protein; ; v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS936810 Testing Data
OMIM
615278
3D Structure
ModBase 3D Structure for P01116
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human KRAS. No significant cross-reactivity or interference between human KRAS and analogues was observed.
Samples
Serum, plasma, tissue homogenates.
Assay Type
Sandwich
Detection Range
23.5 pg/ml -1500 pg/ml.
Sensitivity
5.8 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of KRAS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forKRASpurchase
MBS936810 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KRAS. The ELISA analytical biochemical technique of the MBS936810 kit is based on KRAS antibody-KRAS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KRAS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KRAS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
KRAS elisa kit
Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KRAS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KRAS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRAS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRAS bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of KRAS elisa kit
KRAS elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS936810. Click to request current manual
NCBI/Uniprot data below describe general gene information for KRAS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,656 Da
NCBI Official Full Name
GTPase KRas isoform b
NCBI Official Synonym Full Names
Kirsten rat sarcoma viral oncogene homolog
NCBI Official Symbol
NCBI Official Synonym Symbols
NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
  [Similar Products]
NCBI Protein Information
GTPase KRas; K-Ras 2; c-Ki-ras; oncogene KRAS2; K-ras p21 protein; c-Kirsten-ras protein; PR310 c-K-ras oncogene; transforming protein p21; cellular c-Ki-ras2 proto-oncogene; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog
UniProt Protein Name
GTPase KRas
UniProt Synonym Protein Names
K-Ras 2; Ki-Ras; c-K-ras; c-Ki-rasCleaved into the following chain:GTPase KRas, N-terminally processed
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
KRAS2; RASK2  [Similar Products]
UniProt Entry Name
RASK_HUMAN
NCBI Summary for KRAS
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
UniProt Comments for KRAS
KRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts with PHLPP. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein; G protein, monomeric; Motility/polarity/chemotaxis; Oncoprotein; G protein, monomeric, Ras

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: extrinsic to internal side of plasma membrane; focal adhesion; mitochondrion; membrane; cytoplasm; plasma membrane; cytosol; lipid raft

Molecular Function: protein binding; GDP binding; GTP binding; GMP binding; protein complex binding; LRR domain binding

Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; activation of MAPKK activity; nerve growth factor receptor signaling pathway; response to glucocorticoid stimulus; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; small GTPase mediated signal transduction; positive regulation of cell proliferation; visual learning; negative regulation of neuron apoptosis; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; positive regulation of nitric-oxide synthase activity; MAPKKK cascade; response to mineralocorticoid stimulus; social behavior; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; Ras protein signal transduction; insulin receptor signaling pathway; innate immune response; striated muscle cell differentiation; positive regulation of protein amino acid phosphorylation; actin cytoskeleton organization and biogenesis; vascular endothelial growth factor receptor signaling pathway; blood coagulation; leukocyte migration

Disease: Bladder Cancer; Gastric Cancer, Hereditary Diffuse; Pancreatic Cancer; Noonan Syndrome 3; Leukemia, Acute Myeloid; Lung Cancer; Breast Cancer; Schimmelpenning-feuerstein-mims Syndrome; Cardiofaciocutaneous Syndrome 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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