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ku86 elisa kit :: Rabbit ku86 ELISA Kit

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Catalog # MBS745281
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ku86 elisa kit
Product Name

ku86, ELISA Kit

Full Product Name

Rabbit ku86 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
194364
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
5.0-100ng/mL
Sensitivity
1.0ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of ku86 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ku86purchase
MBS745281 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ku86, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ku86. The ELISA analytical biochemical technique of the MBS745281 kit is based on ku86 antibody-ku86 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ku86 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ku86. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Product Categories/Family for ku86 elisa kit
NCBI/Uniprot data below describe general gene information for ku86. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
82,705 Da
NCBI Official Full Name
Ku86 autoantigen related protein 1, partial
NCBI Official Synonym Full Names
X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
NCBI Official Symbol
XRCC5  [Similar Products]
NCBI Official Synonym Symbols
KU80; KUB2; Ku86; NFIV; KARP1; KARP-1
  [Similar Products]
NCBI Protein Information
X-ray repair cross-complementing protein 5; TLAA; CTC85; CTCBF; nuclear factor IV; Ku autoantigen, 80kDa; DNA repair protein XRCC5; thyroid-lupus autoantigen; 86 kDa subunit of Ku antigen; lupus Ku autoantigen protein p86; Ku86 autoantigen related protein 1; CTC box-binding factor 85 kDa subunit; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit
UniProt Protein Name
X-ray repair cross-complementing protein 5
UniProt Synonym Protein Names
86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; Ku80; Ku86; Lupus Ku autoantigen protein p86; Nuclear factor IV; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
UniProt Gene Name
XRCC5  [Similar Products]
UniProt Synonym Gene Names
G22P2; CTC85; CTCBF; TLAA  [Similar Products]
UniProt Entry Name
XRCC5_HUMAN
NCBI Summary for ku86
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]
UniProt Comments for ku86
Ku80: the 80-kilodalton subunit of the Ku complex, also known as ATP-dependant DNA helicase II. A single stranded DNA-dependent ATP-dependent helicase. It functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70.

Protein type: Nuclear receptor co-regulator; RNA-binding; DNA-binding; Nucleolus; EC 3.6.4.-; EC 3.6.1.-; Helicase

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nucleoplasm; nuclear telomere cap complex; membrane; nuclear chromosome, telomeric region; plasma membrane; nucleolus; nucleus; cytosol

Molecular Function: protein C-terminus binding; ATP-dependent DNA helicase activity; protein binding; DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; telomeric DNA binding; damaged DNA binding; double-stranded telomeric DNA binding; 5'-deoxyribose-5-phosphate lyase activity; ATP binding

Biological Process: response to drug; positive regulation of neurogenesis; viral reproduction; transcription, DNA-dependent; DNA repair; DNA duplex unwinding; double-strand break repair via nonhomologous end joining; DNA recombination; cell proliferation; double-strand break repair; positive regulation of interferon type I production; innate immune response; brain development; negative regulation of transcription, DNA-dependent; telomere maintenance
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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