• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

LAMB2 elisa kit :: Rat Laminin ELISA Kit

Scan QR to view Datasheet
Catalog # MBS824860
Unit / Price
  96 Tests  /  $470 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Laminin (LAMB2), ELISA Kit

Popular Item
Also Known As

Rat Laminin ELISA Kit

Product Synonym Names
LAMS; Laminin subunit beta-2; Laminin B1s chain; Laminin-11 subunit beta; Laminin-14 subunit beta; Laminin-15 subunit beta; Laminin-3 subunit beta; Laminin-4 subunit beta; Laminin-7 subunit beta; Laminin-9 subunit beta; S-laminin subunit beta; S-LAM beta
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
1801
3D Structure
ModBase 3D Structure for P15800
Species Reactivity
Specificity
The Rat Laminin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat Laminin proteins within the range of 156 pg/ml - 10000 pg/ml.
Samples
Cell Culture Supernatants, Serum, Plasma
Sensitivity
10 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LAMB2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forLAMB2purchase
MBS824860 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Laminin (LAMB2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LAMB2. The ELISA analytical biochemical technique of the MBS824860 kit is based on LAMB2 antibody-LAMB2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LAMB2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LAMB2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LAMB2 elisa kit
Principle of the Assay: The Rat Laminin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Laminin in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Rat Laminin coated on a 96-well plate. Standards and samples are pipetted into the wells and Laminin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Laminin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Laminin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2. Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide. The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.
Applications Tested/Suitable for LAMB2 elisa kit
Sandwich ELISA (SE)

Typical Testing Data/Standard Curve (for reference only) of LAMB2 elisa kit
LAMB2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS824860. Click to request current manual
NCBI/Uniprot data below describe general gene information for LAMB2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
196,474 Da
NCBI Official Full Name
laminin subunit beta-2
NCBI Official Synonym Full Names
laminin, beta 2
NCBI Official Symbol
Lamb2  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
laminin subunit beta-2; S-LAM beta; laminin chain B3; laminin chain beta 2; S-laminin subunit beta; laminin-3 subunit beta; laminin-4 subunit beta; laminin-7 subunit beta; laminin-9 subunit beta; laminin-11 subunit beta; laminin-14 subunit beta; laminin-15 subunit beta
UniProt Protein Name
Laminin subunit beta-2
UniProt Synonym Protein Names
Laminin chain B3; Laminin-11 subunit beta; Laminin-14 subunit beta; Laminin-15 subunit beta; Laminin-3 subunit beta; Laminin-4 subunit beta; Laminin-7 subunit beta; Laminin-9 subunit beta; S-laminin subunit beta; S-LAM beta
Protein Family
UniProt Gene Name
Lamb2  [Similar Products]
UniProt Synonym Gene Names
S-LAM beta  [Similar Products]
UniProt Entry Name
LAMB2_RAT
NCBI Summary for LAMB2
a component of the basal lamina; may be involved in motor neuron function [RGD, Feb 2006]
UniProt Comments for LAMB2
LAMB2: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Defects in LAMB2 are the cause of Pierson syndrome (PIERSS); also known as microcoria-congenital nephrotic syndrome. Pierson syndrome is characterized by nephrotic syndrome with neonatal onset, diffuse mesangial sclerosis and eye abnormalities with microcoria as the leading clinical feature. Death usually occurs within the first weeks of life. Disease severity depends on the mutation type: nontruncating LAMB2 mutations may display variable phenotypes ranging from a milder variant of Pierson syndrome to isolated congenital nephrotic syndrome. Defects in LAMB2 are the cause of nephrotic syndrome type 5 with or without ocular abnormalities (NPHS5). NPHS5 is a renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. NPHS5 is characterized by very early onset of progressive renal failure. A subset of patients may develop mild ocular anomalies, such as myopia, nystagmus, and strabismus.

Protein type: Secreted; Extracellular matrix; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular matrix; laminin-3 complex; basement membrane; basal lamina; synapse

Molecular Function: integrin binding

Biological Process: cellular morphogenesis during differentiation; axon guidance; Schwann cell development; visual perception; retina development in camera-type eye; axon extension involved in regeneration; synapse organization and biogenesis; cell adhesion; astrocyte development; neurite development; neuromuscular junction development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000