• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

LAMC2 elisa kit :: Human Laminin ELISA Kit

Scan QR to view Datasheet
Catalog # MBS355438
Unit / Price
  96 Tests  /  $440 +1 FREE 8GB USB
Typical Testing Data/Standard Curve
Product Name

Laminin (LAMC2), ELISA Kit

Popular Item
Full Product Name

Laminin Human ELISA Kit

Product Synonym Names
Human Laminin
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 1; NC_000001.10 (183147952..183214262). Location: 1q25-q31
OMIM
150292
3D Structure
ModBase 3D Structure for Q13753
Species Reactivity
Samples
Human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Assay Type
Quantitative Sandwich
Sensitivity
< 10 pg/ml
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LAMC2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forLAMC2purchase
MBS355438 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Laminin (LAMC2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LAMC2. The ELISA analytical biochemical technique of the MBS355438 kit is based on LAMC2 antibody-LAMC2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LAMC2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LAMC2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LAMC2 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Laminin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Laminin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Laminin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Laminin can be calculated.

Background/Introduction: Laminins are major proteins in the basal lamina, a protein network foundation for most cells and organs. Fifteen laminin trimers have been identified. The laminins are combinations of different alpha-, beta-, and gamma-chains. The laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, adhesion as well as phenotype and survival. Recently, several publications have demonstrated that laminins can be used to culture cells, such as pluripotent stem cells, that are difficult to culture on other substrates. Rodin et al. showed that recombinant laminin 511 can be used to create a totally xeno-free and defined cell culture environment to culture human pluripotent ES cells and human iPS cells.
Applications Tested/Suitable for LAMC2 elisa kit
Quantitative sELISA (EIA)
Application Notes for LAMC2 elisa kit
For quantitative detection of Laminin in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.

Typical Testing Data/Standard Curve of LAMC2 elisa kit
LAMC2 elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS355438. Click to request current manual
NCBI/Uniprot data below describe general gene information for LAMC2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
130,976 Da[Similar Products]
NCBI Official Full Name
Laminin
NCBI Official Synonym Full Names
laminin, gamma 2
NCBI Official Symbol
LAMC2  [Similar Products]
NCBI Official Synonym Symbols
B2T; CSF; EBR2; BM600; EBR2A; LAMB2T; LAMNB2
  [Similar Products]
NCBI Protein Information
laminin subunit gamma-2; BM600-100kDa; laminin B2t chain; CSF 140 kDa subunit; nicein subunit gamma; kalinin subunit gamma; ladsin 140 kDa subunit; epiligrin subunit gamma; cell-scattering factor 140 kDa subunit; large adhesive scatter factor 140 kDa subunit
UniProt Protein Name
Laminin subunit gamma-2
UniProt Synonym Protein Names
Cell-scattering factor 140 kDa subunit; CSF 140 kDa subunit; Epiligrin subunit gamma; Kalinin subunit gamma; Kalinin/nicein/epiligrin 100 kDa subunit; Ladsin 140 kDa subunit; Laminin B2t chain; Laminin-5 subunit gamma; Large adhesive scatter factor 140 kDa subunit; Nicein subunit gamma
Protein Family
UniProt Gene Name
LAMC2  [Similar Products]
UniProt Synonym Gene Names
LAMB2T; LAMNB2; CSF 140 kDa subunit  [Similar Products]
UniProt Entry Name
LAMC2_HUMAN
NCBI Summary for LAMC2
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature. [provided by RefSeq, Aug 2011]
UniProt Comments for LAMC2
Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ladsin exerts cell-scattering activity toward a wide variety of cells, including epithelial, endothelial, and fibroblastic cells. Ref.7

Subunit structure: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Gamma-2 is a subunit of laminin-5 (laminin-332 or epiligrin/kalinin/nicein).

Subcellular location: Secreted › extracellular space › extracellular matrix › basement membrane. Note: Major component.

Tissue specificity: The large variant is expressed only in specific epithelial cells of embryonic and neonatal tissues. In 17-week old embryo the small variant is found in cerebral cortex, lung, and distal tubes of kidney, but not in epithelia except for distal tubuli.

Domain: The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.Domain IV is globular.

Involvement in disease: Epidermolysis bullosa, junctional, Herlitz type (H-JEB) [MIM:226700]: An infantile and lethal form of junctional epidermolysis bullosa, a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement In the Herlitz type, death occurs usually within the first six months of life. Occasionally, children survive to teens. It is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic.Note: The disease is caused by mutations affecting the gene represented in this entry.

Miscellaneous: Binds heparin

By similarity.

Sequence similarities: Contains 8 laminin EGF-like domains.Contains 1 laminin IV type A domain.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000