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lasA recombinant protein :: Protease lasA (lasA) Recombinant Protein

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Catalog # MBS1185217
Unit / Price
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  0.01 mg (E-Coli)  /  $235 +1 FREE 8GB USB
  0.05 mg (E-Coli)  /  $320 +1 FREE 8GB USB
  0.1 mg (E-Coli)  /  $535 +1 FREE 8GB USB
  0.2 mg (E-Coli)  /  $855 +1 FREE 8GB USB
  0.5 mg (E-Coli)  /  $1,125 +1 FREE 8GB USB
  1 mg (E-Coli)  /  $1,725 +2 FREE 8GB USB
SDS-PAGE
Product Name

Protease lasA (lasA), Recombinant Protein

Popular Item
Full Product Name

Recombinant Pseudomonas aeruginosa Protease lasA (lasA)

Product Synonym Names
Protease lasA; EC=3.4.24.-; Staphylolytic protease
Product Synonym Gene Name
lasA; PA1871[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
237-418. Full length of the mature protein.
Sequence
APPSNLMQLP WRQGYSWQPN GAHSNTGSGY PYSSFDASYD WPRWGSATYS VVAAHAGTVR VLSRCQVRVT HPSGWATNYY HMDQIQVSNG QQVSADTKLG VYAGNINTAL CEGGSSTGPH LHFSLLYNGA FVSLQGASFG PYRINVGTSN YDNDCRRYYF YNQSAGTTHC AFRPLYNPGL AL
3D Structure
ModBase 3D Structure for P14789
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>=90% (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Species
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of lasA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
lasA recombinant protein
Involved in proteolysis and elastolysis (degradation of the host protein elastin). Has staphylolytic activity (degrades pentaglycine cross-links in cell wall peptidogylcan), preferring Gly-Gly-|-X substrates where X is Ala or Gly (PubMed:11179971). Enhances the elastolytic but not proteolytic activity of elastase (lasB) and elastolytic activity of other proteases (PubMed:2110137). Degradation of host elastin is likely to contribute to the pathogenicity of P.aeruginosa. While either His-317 or His-356 can abstract a proton in the hydrolysis reaction, the same residue performs both functions in a given catalytic cycle, with the other stabilizing the catalytic intermediate (PubMed:20026068).

lasA recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
NCBI/Uniprot data below describe general gene information for lasA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
35kD
NCBI Official Full Name
LasA protease
NCBI Official Symbol
NCBI Protein Information
LasA protease
UniProt Protein Name
Protease LasA
UniProt Synonym Protein Names
Staphylolytic protease
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
PA1871  [Similar Products]
UniProt Entry Name
LASA_PSEAE
UniProt Comments for lasA
Involved in proteolysis and elastolysis (degradation of the host protein elastin). Has staphylolytic activity (degrades pentaglycine cross-links in cell wall peptidogylcan), preferring Gly-Gly-|-X substrates where X is Ala or Gly (PubMed:11179971). Enhances the elastolytic but not proteolytic activity of elastase (lasB) and elastolytic activity of other proteases (PubMed:2110137). Degradation of host elastin is likely to contribute to the pathogenicity of P.aeruginosa. While either His-317 or His-356 can abstract a proton in the hydrolysis reaction, the same residue performs both functions in a given catalytic cycle, with the other stabilizing the catalytic intermediate (PubMed:20026068).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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