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LAT1 elisa kit :: Human Large Neutral Amino Acid Transporter 1 (LAT1) ELISA Kit

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Catalog # MBS2022184 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $270 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $665 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,715 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,895 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Large Neutral Amino Acid Transporter 1 (LAT1), ELISA Kit

Popular Item
Also Known As

Large Neutral Amino Acid Transporter 1 (LAT1) ELISA Kit

Product Synonym Names
CD98; MDU1; 4T2HC; 4F2; NACAE; SLC7A5; MPE16; Solute Carrier Family 7 Member 5; Cationic Amino Acid Transporter,y+ System; L-Type Amino Acid Transporter
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
507
OMIM
600182
3D Structure
ModBase 3D Structure for Q01650
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of LAT1. No significant cross-reactivity or interference between LAT1 and analogues was observed.
Samples
Tissue homogenates, cell lysates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.058ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LAT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LAT1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LAT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forLAT1purchase
MBS2022184 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Large Neutral Amino Acid Transporter 1 (LAT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LAT1. The ELISA analytical biochemical technique of the MBS2022184 kit is based on LAT1 antibody-LAT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LAT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LAT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LAT1 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LAT1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to LAT1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to LAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LAT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of LAT1 elisa kit
LAT1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2022184. Click to request current manual
NCBI/Uniprot data below describe general gene information for LAT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
55,010 Da
NCBI Official Full Name
large neutral amino acids transporter small subunit 1
NCBI Official Synonym Full Names
solute carrier family 7 (amino acid transporter light chain, L system), member 5
NCBI Official Symbol
SLC7A5  [Similar Products]
NCBI Official Synonym Symbols
E16; CD98; LAT1; 4F2LC; MPE16; hLAT1; D16S469E
  [Similar Products]
NCBI Protein Information
large neutral amino acids transporter small subunit 1
UniProt Protein Name
Large neutral amino acids transporter small subunit 1
UniProt Synonym Protein Names
4F2 light chain; 4F2 LC; 4F2LC; CD98 light chain; Integral membrane protein E16; L-type amino acid transporter 1; hLAT1; Solute carrier family 7 member 5; y+ system cationic amino acid transporter
UniProt Gene Name
SLC7A5  [Similar Products]
UniProt Synonym Gene Names
CD98LC; LAT1; MPE16; 4F2 LC; 4F2LC; hLAT1  [Similar Products]
UniProt Entry Name
LAT1_HUMAN
UniProt Comments for LAT1
SLC7A5: Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood- brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L- nitrosocysteine (L-CNSO) across the transmembrane. May play an important role in high-grade gliomas. Mediates blood-to-retina L- leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts. Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc. Expression induced in quiescent peripheral blood lymphocytes after treatment with phorbol myristate acetate (PMA) and phytohemagglutinin (PHA). Expression and the uptake of leucine is stimulated in mononuclear, cytotrophoblast-like choriocarcinoma cells by combined treatment with PMA and calcium ionophore. Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2. Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family; Transporter

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: intracellular membrane-bound organelle; membrane; cytoplasm; apical plasma membrane; integral to membrane; plasma membrane; nucleolus; cytosol; nucleus

Molecular Function: L-amino acid transmembrane transporter activity; peptide antigen binding; neutral amino acid transmembrane transporter activity; amino acid transmembrane transporter activity

Biological Process: neutral amino acid transport; amino acid metabolic process; nervous system development; amino acid transport; transport; ion transport; blood coagulation; cell differentiation; transmembrane transport; leukocyte migration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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