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LATS2 recombinant protein

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Catalog # MBS515127
Unit / Price
  0.02 mg  /  $270 +1 FREE 8GB USB
  0.05 mg  /  $450 +1 FREE 8GB USB
SDS-PAGE
Product Name

LATS2, Recombinant Protein

Popular Item
Full Product Name

LATS2, Unactive recombinant protein

Product Synonym Names
KPM; FLJ13161
Product Synonym Gene Name
KPM; FLJ13161[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
480-1088
Chromosome Location
Chromosome: 13; NC_000013.10 (21547175..21635722, complement). Location: 13q11-q12
OMIM
604861
3D Structure
ModBase 3D Structure for Q9NRM7
Host
Sf9 insect cells
Purity/Purification
>80%
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.2ug/ul (lot specific)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant human LATS2 (480-1088) was expressed by baculovirus in Sf9 insect cells
Preparation and Storage
Stability: 1yr At –70oC from date of shipment

Storage & Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Shipping: ships on dry ice.
Other Notes
Small volumes of LATS2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
LATS2 recombinant protein
Recombinant human LATS2 (480-1088) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: LATS2 is a serine/threonine protein kinase belonging to the LATS tumor suppressor family (1). The kinase activity and two conserved domains within LATS2 are responsible for the suppression of tumorigenicity and inhibition of cell growth. LATS2 negatively regulates the cell cycle by controlling G1/S and/or G2/M transition. LATS2 interacts with the centrosomal proteins AURORA A and Ajuba and is required for accumulation of γ-tubulin and spindle formation at the onset of mitosis (2). LATS2 also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. LATS2 can also function as a co-repressor of androgen-responsive gene expression.
Applications Tested/Suitable for LATS2 recombinant protein
Kinase Assay, Western Blot (WB)

The purity was determined to be >80% by densitometry.
Approx. MW 95kDa
LATS2 recombinant protein SDS-PAGE image
NCBI/Uniprot data below describe general gene information for LATS2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
~98 kDa
NCBI Official Full Name
Homo sapiens large tumor suppressor kinase 2 (LATS2), mRNA
NCBI Official Synonym Full Names
large tumor suppressor kinase 2
NCBI Official Symbol
LATS2  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
serine/threonine-protein kinase LATS2; warts-like kinase; serine/threonine kinase KPM; large tumor suppressor homolog 2; serine/threonine-protein kinase kpm; LATS, large tumor suppressor, homolog 2; kinase phosphorylated during mitosis protein; LATS (large tumor suppressor, Drosophila) homolog 2
UniProt Protein Name
Serine/threonine-protein kinase LATS2
UniProt Synonym Protein Names
Kinase phosphorylated during mitosis protein; Large tumor suppressor homolog 2; Serine/threonine-protein kinase kpm; Warts-like kinase
UniProt Gene Name
LATS2  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
LATS2_HUMAN
NCBI Summary for LATS2
This gene encodes a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression. [provided by RefSeq, Jul 2008]
UniProt Comments for LATS2
Function: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ. Ref.1 Ref.6 Ref.8 Ref.11 Ref.15

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Subunit structure: Interacts with and is phosphorylated by AURKA. Binds to AR. Interacts with AJUBA during mitosis and this complex regulates organization of the spindle apparatus through recruitment of gamma-tubulin to the centrosome. Interacts (via PPxY motif) with YAP1 (via WW domains). Interacts with MOB1A and MOB1B. Interacts with LIMD1, WTIP and AJUBA. Interacts with SNAI1. Ref.7 Ref.8 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15

Subcellular location: Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cytoplasm. Cytoplasm › cytoskeleton › spindle pole. Nucleus. Note: Colocalizes with AURKA at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis. Translocates to the nucleus upon mitotic stress by nocodazole treatment. Ref.6 Ref.7 Ref.15

Tissue specificity: Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined. Ref.1 Ref.5

Post-translational modification: Autophosphorylated and phosphorylated during M-phase and the G1/S-phase of the cell cycle. Phosphorylated and activated by STK3/MST2. Ref.1 Ref.7 Ref.9 Ref.15

Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.Contains 1 AGC-kinase C-terminal domain.Contains 1 protein kinase domain.Contains 1 UBA domain.

Sequence caution: The sequence BAA92381.1 differs from that shown. Reason: Frameshift at positions 512 and 526.
Product References and Citations for LATS2 recombinant protein
1. Li, Y. et al: Lats2, a putative tumor suppressor, inhibits G1/S transition. Oncogene. 2003; 22(28):4398-405.
2. Yabuta, N. et al: Structure, expression, and chromosome mapping of LATS2, a mammalian homologue of the Drosophila tumor suppressor gene lats/warts. Genomics. 2000 63(2):263-70.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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