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LDHB elisa kit :: Human lactate dehydrogenase B ELISA Kit

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Catalog # MBS928418
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

lactate dehydrogenase B (LDHB), ELISA Kit

Popular Item
Full Product Name

Human L-lactate dehydrogenase B chain, LDHB ELISA Kit

Product Synonym Names
Human L-lactate dehydrogenase B chain (LDHB) ELISA kit; LDH-H; TRG-5; L-lactate dehydrogenase B; OTTHUMP00000165229; lactate dehydrogenase B
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS928418 Testing Data
OMIM
gene 614128
3D Structure
ModBase 3D Structure for P07195
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human LDHB. No significant cross-reactivity or interference between human LDHB and analogues was observed.
Samples
Serum, plasma, urine, cerebrospinal fluid (CSF), tissue homogenates
Assay Type
Sandwich
Detection Range
23.5 mU/ml-1500 mU/ml.
Sensitivity
The minimum detectable dose of human LDHB is typically less than 5.8 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LDHB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forLDHBpurchase
MBS928418 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the lactate dehydrogenase B (LDHB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LDHB. The ELISA analytical biochemical technique of the MBS928418 kit is based on LDHB antibody-LDHB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LDHB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LDHB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LDHB elisa kit
Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LDHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LDHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LDHB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDHB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of LDHB elisa kit
LDHB elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS928418. Click to request current manual
NCBI/Uniprot data below describe general gene information for LDHB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
36,638 Da
NCBI Official Full Name
L-lactate dehydrogenase B chain
NCBI Official Synonym Full Names
lactate dehydrogenase B
NCBI Official Symbol
NCBI Official Synonym Symbols
LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
  [Similar Products]
NCBI Protein Information
L-lactate dehydrogenase B chain; LDH heart subunit; lactate dehydrogenase H chain; renal carcinoma antigen NY-REN-46; epididymis secretory protein Li 281
UniProt Protein Name
L-lactate dehydrogenase B chain
UniProt Synonym Protein Names
LDH heart subunit; LDH-H; Renal carcinoma antigen NY-REN-46
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
LDH-B; LDH-H  [Similar Products]
UniProt Entry Name
LDHB_HUMAN
NCBI Summary for LDHB
This gene encodes an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2010]
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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