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LDHB elisa kit :: Human LDHB ELISA Kit

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Catalog # MBS2500565 (SPECIAL PROMOTIONAL PRICING for a limited time)
Unit / Price
  48-Strip-Wells  /  $310 +1 FREE 8GB USB
  96-Strip-Wells  /  $355 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,435 +1 FREE 8GB USB
  10x96-Strip-Wells  /  $2,805 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

LDHB, ELISA Kit

Popular Item
Full Product Name

Human LDHB (Lactate Dehydrogenase B) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2500565 COA
OMIM
150100
3D Structure
ModBase 3D Structure for P07195
Species Reactivity
Specificity
This kit recognizes natural and recombinant Human LDHB. No significant cross-reactivity or interference between Human LDHB and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
3.125-200ng/mL
Sensitivity
Min: 1.875ng/mL; Max: 200ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LDHB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forLDHBpurchase
MBS2500565 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the LDHB, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LDHB. The ELISA analytical biochemical technique of the MBS2500565 kit is based on LDHB antibody-LDHB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LDHB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LDHB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LDHB elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human LDHB concentrations in serum, plasma and other biological fluids.

Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LDHB. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LDHB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LDHB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of LDHB. You can calculate the concentration of LDHB in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of LDHB elisa kit
LDHB elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2500565. Click to request current manual
NCBI/Uniprot data below describe general gene information for LDHB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
36,638 Da
NCBI Official Full Name
L-lactate dehydrogenase B chain
NCBI Official Synonym Full Names
lactate dehydrogenase B
NCBI Official Symbol
NCBI Official Synonym Symbols
LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
  [Similar Products]
NCBI Protein Information
L-lactate dehydrogenase B chain; LDH heart subunit; epididymis secretory protein Li 281; lactate dehydrogenase H chain; renal carcinoma antigen NY-REN-46
UniProt Protein Name
L-lactate dehydrogenase B chain
UniProt Synonym Protein Names
LDH heart subunit; LDH-H; Renal carcinoma antigen NY-REN-46
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
LDH-B; LDH-H  [Similar Products]
UniProt Entry Name
LDHB_HUMAN
NCBI Summary for LDHB
This gene encodes an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2010]
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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