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LDHC elisa kit :: Human lactate dehydrogenase C ELISA Kit

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Catalog # MBS906075
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Typical Testing Data/Standard Curve (for reference only)
Product Name

lactate dehydrogenase C (LDHC), ELISA Kit

Popular Item
Full Product Name

Human L-lactate dehydrogenase C chain, LDHC ELISA Kit

Product Synonym Names
Human L-lactate dehydrogenase C chain (LDHC) ELISA kit; CT32; LDH3; LDHX; MGC111073; L-lactate dehydrogenase C; cancer/testis antigen 32; lactate dehydrogenase C
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS906075 Testing Data
3D Structure
ModBase 3D Structure for P07864
Species Reactivity
This assay has high sensitivity and excellent specificity for detection of human LDHC. No significant cross-reactivity or interference between human LDHC and analogues was observed.
Serum, plasma, tissue homogenates, urine
Assay Type
Detection Range
6.25 mU/ml-400 mU/ml.
The minimum detectable dose of human LDHC is typically less than 1.56 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LDHC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for LDHCpurchase
MBS906075 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the lactate dehydrogenase C (LDHC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LDHC. The ELISA analytical biochemical technique of the MBS906075 kit is based on LDHC antibody-LDHC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LDHC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LDHC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LDHC elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LDHC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LDHC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LDHC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDHC bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of LDHC elisa kit
LDHC elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS906075. Click to request current manual
NCBI/Uniprot data below describe general gene information for LDHC. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
36,311 Da
NCBI Official Full Name
L-lactate dehydrogenase C chain
NCBI Official Synonym Full Names
lactate dehydrogenase C
NCBI Official Symbol
NCBI Official Synonym Symbols
  [Similar Products]
NCBI Protein Information
L-lactate dehydrogenase C chain; LDH-C; LDH-X; LDH testis subunit; cancer/testis antigen 32; lactate dehydrogenase C4; lactate dehydrogenase c variant 1; lactate dehydrogenase c variant 3; lactate dehydrogenase c variant 4
UniProt Protein Name
L-lactate dehydrogenase C chain
UniProt Synonym Protein Names
Cancer/testis antigen 32; CT32; LDH testis subunit; LDH-X
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
LDH3; LDHX; LDH-C; CT32  [Similar Products]
UniProt Entry Name
NCBI Summary for LDHC
Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region. [provided by RefSeq, Jul 2008]
UniProt Comments for LDHC
LDH-C: Possible role in sperm motility. Belongs to the LDH/MDH superfamily. LDH family.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - cysteine and methionine; Cancer Testis Antigen (CTA); Oxidoreductase

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: cytoplasm; nucleus

Molecular Function: L-lactate dehydrogenase activity

Biological Process: sperm motility; ATP biosynthetic process; cellular carbohydrate metabolic process; lactate oxidation; lactate biosynthetic process from pyruvate
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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