ELISA is a popular format of “wet-lab” type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample.<\/p>\n[\/et_pb_blurb][et_pb_blurb title=”Western Blotting” url=”https:\/\/www.mybiosource.com\/learn\/westernblotting” image=”https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2018\/04\/coding-icon_1.jpg” icon_placement=”left” content_max_width=”1100px” _builder_version=”4.24.0″ header_level=”h3″ header_font=”|on|||” header_text_color=”#2e2545″ header_line_height=”1.5em” body_text_color=”#000000″ body_line_height=”1.9em” background_color=”#ffffff” background_size=”initial” background_position=”top_left” background_repeat=”repeat” custom_margin=”||10%|” custom_padding=”30px|40px|30px|40px” animation_direction=”bottom” box_shadow_style=”preset2″ box_shadow_horizontal=”0px” box_shadow_vertical=”0px” box_shadow_blur=”60px” box_shadow_color=”rgba(71,74,182,0.12)” locked=”off” global_colors_info=”{}”]\n
Blotting is used in molecular biology for the identification of proteins and nucleic acids and is widely used for diagnostic purposes. This technique immobilizes the molecule of interest on a support, which is a nitrocellulosic membrane or nylon. It uses hybridization techniques for the identification of the specific nucleic acids and genes.<\/p>\n[\/et_pb_blurb][et_pb_blurb title=”Southern Blotting” url=”https:\/\/www.mybiosource.com\/learn\/southern-blotting\/” image=”https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2018\/04\/coding-icon_3.jpg” icon_placement=”left” content_max_width=”1100px” _builder_version=”4.24.0″ header_level=”h3″ header_font=”|on|||” header_text_color=”#2e2545″ header_line_height=”1.5em” body_text_color=”#000000″ body_line_height=”1.9em” background_color=”#ffffff” background_size=”initial” background_position=”top_left” background_repeat=”repeat” custom_margin=”||10%|” custom_padding=”30px|40px|30px|40px” animation_direction=”bottom” box_shadow_style=”preset2″ box_shadow_horizontal=”0px” box_shadow_vertical=”0px” box_shadow_blur=”60px” box_shadow_color=”rgba(71,74,182,0.12)” locked=”off” global_colors_info=”{}”]\n
The first of these techniques developed was the Southern blot, named after Dr. Edwin Southern who developed it to identify specific DNA sequences. Southern blotting is a detection technique used to find the target DNA sequences in the DNA sample in the field of molecular biology. The process starts from electrophoresis of DNA molecules which are hybridized in a blotting membrane followed by a transfer step where DNA from gel is transferred onto the blotting membrane.<\/p>\n[\/et_pb_blurb][et_pb_blurb title=”Gene Knockout” url=”#” image=”https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2018\/04\/coding-icon_5.jpg” icon_placement=”left” content_max_width=”1100px” _builder_version=”4.24.0″ header_level=”h3″ header_font=”|on|||” header_text_color=”#2e2545″ header_line_height=”1.5em” body_text_color=”#000000″ body_line_height=”1.9em” background_color=”#ffffff” background_size=”initial” background_position=”top_left” background_repeat=”repeat” custom_margin=”||10%|” custom_padding=”30px|40px|30px|40px” animation_direction=”bottom” box_shadow_style=”preset2″ box_shadow_horizontal=”0px” box_shadow_vertical=”0px” box_shadow_blur=”60px” box_shadow_color=”rgba(71,74,182,0.12)” locked=”off” global_colors_info=”{}”]\n
When a mutation inactivates a gene function it is called as gene knockout. Gene knockout has its application both in classical genetics and modern techniques such as functional genomics. During the initial timescale, gene knockout was carried n transposon mutagenesis. The major disadvantage of that method is the laborious screening to find the knocked out gene.<\/p>\n[\/et_pb_blurb][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_blurb title=”Branched DNA Assay” url=”https:\/\/www.mybiosource.com\/learn\/branched-dna-bdna-technology\/” image=”https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2018\/04\/coding-icon_7.jpg” icon_placement=”left” content_max_width=”1100px” _builder_version=”4.24.0″ header_level=”h3″ header_font=”|on|||” header_text_color=”#2e2545″ header_line_height=”1.5em” body_text_color=”#000000″ body_line_height=”1.9em” background_color=”#ffffff” background_size=”initial” background_position=”top_left” background_repeat=”repeat” custom_margin=”-80px||10%|” custom_margin_tablet=”0px||10%|” custom_margin_phone=”” custom_margin_last_edited=”on|phone” custom_padding=”30px|40px|30px|40px” animation_direction=”bottom” box_shadow_style=”preset2″ box_shadow_horizontal=”0px” box_shadow_vertical=”0px” box_shadow_blur=”60px” box_shadow_color=”rgba(71,74,182,0.12)” locked=”off” global_colors_info=”{}”]\n
The branched DNA (bDNA) assay provides a unique and powerful tool for reliable quantification of nucleic acid molecules. Fundamentally different from target amplification methods such as PCR, the bDNA assay directly measures nucleic acid molecules at physiological levels by boosting the reporter signal, rather than replicating target sequences as the means of detection, and hence avoids the errors inherent in the extraction and amplification of target sequences.<\/p>\n[\/et_pb_blurb][et_pb_blurb title=”Polymerase Chain Reaction” url=”https:\/\/www.mybiosource.com\/learn\/polymerase-chain-reaction\/” image=”https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2018\/04\/coding-icon_2.jpg” icon_placement=”left” content_max_width=”1100px” _builder_version=”4.24.0″ header_level=”h3″ header_font=”|on|||” header_text_color=”#2e2545″ header_line_height=”1.5em” body_text_color=”#000000″ body_line_height=”1.9em” background_color=”#ffffff” background_size=”initial” background_position=”top_left” background_repeat=”repeat” custom_margin=”||10%|” custom_padding=”30px|40px|30px|40px” animation_direction=”bottom” box_shadow_style=”preset2″ box_shadow_horizontal=”0px” box_shadow_vertical=”0px” box_shadow_blur=”60px” box_shadow_color=”rgba(71,74,182,0.12)” locked=”off” global_colors_info=”{}”]\n
PCR is a technology which has its application in all molecular biology applications. The advent of PCR meant that insufficiencies in the quantity of DNA were no longer a limitation in molecular biology research or diagnostic procedures. The chemistry involved in PCR depends on the complementarity (matching) of the nucleotide bases in the double-stranded DNA helix. When a molecule of DNA is sufficiently heated, the hydrogen bonds holding together the double helix are disrupted and the molecule separates or denatures into single strands. If the DNA solution is allowed to cool, the complementary base pairs can reform to restore the original double helix.<\/p>\n[\/et_pb_blurb][et_pb_blurb title=”Gene Transfer Technique” url=”https:\/\/www.mybiosource.com\/learn\/gene-transfer-technique\/” image=”https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2018\/04\/coding-icon_4.jpg” icon_placement=”left” content_max_width=”1100px” _builder_version=”4.24.0″ header_level=”h3″ header_font=”|on|||” header_text_color=”#2e2545″ header_line_height=”1.5em” body_text_color=”#000000″ body_line_height=”1.9em” background_color=”#ffffff” background_size=”initial” background_position=”top_left” background_repeat=”repeat” custom_margin=”||10%|” custom_padding=”30px|40px|30px|40px” animation_direction=”bottom” box_shadow_style=”preset2″ box_shadow_horizontal=”0px” box_shadow_vertical=”0px” box_shadow_blur=”60px” box_shadow_color=”rgba(71,74,182,0.12)” locked=”off” global_colors_info=”{}”]\n
Gene transfer techniques for higher plants and animals are complex and costly. It is generally not done in laboratory scale experiment. However, the techniques of gene transfer in bacteria like\u00a0Escherichia coli<\/em>\u00a0(E. coli) are simple and appropriate for the teaching and learning laboratory. Plasmid-based genetic transformation is the most common used technology for genetic transfer.<\/p>\n[\/et_pb_blurb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ admin_label=”Footer” _builder_version=”4.16″ background_color=”#f7f8fc” custom_padding=”0px|0px|100px|0px” animation_style=”zoom” animation_direction=”bottom” animation_intensity_zoom=”6%” animation_starting_opacity=”100%” saved_tabs=”all” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ background_size=”initial” background_position=”top_left” background_repeat=”repeat” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ custom_padding=”|||” global_colors_info=”{}” custom_padding__hover=”|||”][et_pb_text _builder_version=”4.16″ global_colors_info=”{}”]\n MyBioSource Life Science Resources MyBioSource, Inc. is a biotechnological products distribution company formed to create a large portfolio of laboratory research reagents, both hard to find and common items, with worldwide distributions. ELISA is a popular format of “wet-lab” type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of […]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-1856","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/pages\/1856","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/comments?post=1856"}],"version-history":[{"count":4,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/pages\/1856\/revisions"}],"predecessor-version":[{"id":9653,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/pages\/1856\/revisions\/9653"}],"wp:attachment":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/media?parent=1856"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}Recent Publications<\/h2>\n[\/et_pb_text][et_pb_blog fullwidth=”off” posts_number=”25″ include_categories=”31,2,32,18″ show_author=”off” show_date=”off” show_categories=”off” show_pagination=”off” _builder_version=”4.24.0″ header_level=”h3″ header_font=”Montserrat|600|||||||” header_text_align=”center” body_font=”Bitter||||||||” text_orientation=”justified” pagination_font_size_tablet=”51″ pagination_line_height_tablet=”2″ global_colors_info=”{}”][\/et_pb_blog][\/et_pb_column][\/et_pb_row][\/et_pb_section]\n","protected":false},"excerpt":{"rendered":"