{"id":5347,"date":"2022-09-17T10:27:11","date_gmt":"2022-09-17T10:27:11","guid":{"rendered":"https:\/\/www.mybiosource.com\/learn\/?page_id=5347"},"modified":"2023-03-02T13:14:43","modified_gmt":"2023-03-02T13:14:43","slug":"elisa-formats","status":"publish","type":"page","link":"https:\/\/www.mybiosource.com\/learn\/elisa-formats\/","title":{"rendered":"ELISA Formats"},"content":{"rendered":"<h2><strong>Who invented ELISA?<\/strong><\/h2>\n<p>Two different teams led by Stratis Avrameas and G. B. Pierce in the 1960s laid the groundwork for the <span id=\"urn:enhancement-b7075c48-8a59-4ca5-9001-d295d6161284\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/enzyme\">enzyme<\/span> linking process. In the same period, immunosorbent preparation techniques were published by Wide and <span id=\"urn:enhancement-b798eb0a-c38f-4d8b-a561-d0a44c3b362a\" class=\"textannotation disambiguated wl-person\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/jerker-porath\">Jerker Porath<\/span>.\u00a0Following which, scientists in Sweden (Peter Perlmann and <span id=\"urn:enhancement-d8a42c6c-57a2-4685-a7f7-df22a6a4ec11\" class=\"textannotation disambiguated wl-person\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/eva-engvall\">Eva Engvall<\/span> at <span id=\"urn:enhancement-a817fe3f-8432-494d-8b81-01a2beb7624f\" class=\"textannotation disambiguated wl-organization\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/stockholm-university\">Stockholm University<\/span>) and the Netherlands (<span id=\"urn:enhancement-82fb1b3d-82be-4ebe-99c8-89a68bde79e2\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/anton\">Anton<\/span> Schuurs and Bauke van Weemen) generated knowledge that goes into making ELISAs.<\/p>\n<p>Before <span id=\"urn:enhancement-7176c7f4-d4c6-488f-81bd-0602aedf0859\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/elisa\">ELISA<\/span>, radioimmunoassay employing radioactively labeled antigens and antibodies were used. <span id=\"urn:enhancement-1c9c901d-b2fc-4f39-aa0a-7480c749711f\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/radioactivity\">Radioactivity<\/span> served as the reporter signal indicating specific <span id=\"urn:enhancement-63f462c1-0644-4bd6-be38-dc981bc77898\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/antigen\">antigen<\/span> or antibody. As radioimmunoassay posed significant health risks to researchers, <span id=\"urn:enhancement-fbc9ac7a-76fc-455a-8acd-426216927555\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/alternatives\">alternatives<\/span> were sought.<\/p>\n<h2><strong>H2: What is the principle behind ELISA?<\/strong><\/h2>\n<p><span id=\"urn:enhancement-058d2fb9-ce39-47a2-b1e9-163f0a14deb1\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/elisa\">ELISA<\/span> works by coupling antibodies or <span id=\"urn:enhancement-0b7a3cba-e2ea-4e81-abee-1e33c3eabd8d\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/antigen\">antigen<\/span> to assay <span id=\"urn:enhancement-75947687-4504-4fec-a9f3-6d355193b7d1\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/enzyme\">enzyme<\/span>. The assay combines the specificity of antibody and sensitivity of assay enzymes to primarily detect antigens through assay antibodies or antibodies through assay antigens.<\/p>\n<p>The sensitivity and <span id=\"urn:enhancement-99e9ab34-5290-4454-aadf-a5159ca8435c\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/precision\">precision<\/span> of the assay is <span id=\"urn:enhancement-72356b0f-9b1f-4b89-89e3-45720f180264\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/enhanced\">enhanced<\/span> by coating the plate with high-<span id=\"urn:enhancement-414faed5-be98-4556-8866-5a223ded9d42\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/affinity\">affinity<\/span> antibodies.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Who invented ELISA? Two different teams led by Stratis Avrameas and G. B. Pierce in the 1960s laid the groundwork for the enzyme linking process. In the same period, immunosorbent preparation techniques were published by Wide and Jerker Porath.\u00a0Following which, scientists in Sweden (Peter Perlmann and Eva Engvall at Stockholm University) and the Netherlands (Anton [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-5347","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/pages\/5347","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/comments?post=5347"}],"version-history":[{"count":0,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/pages\/5347\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/media?parent=5347"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}