{"id":8701,"date":"2023-04-16T18:26:01","date_gmt":"2023-04-16T18:26:01","guid":{"rendered":"https:\/\/www.mybiosource.com\/learn\/?page_id=8701"},"modified":"2023-04-17T04:39:14","modified_gmt":"2023-04-17T04:39:14","slug":"future-directions-and-innovations-in-pcr-technology","status":"publish","type":"page","link":"https:\/\/www.mybiosource.com\/learn\/future-directions-and-innovations-in-pcr-technology\/","title":{"rendered":"Future Directions and Innovations in PCR Technology"},"content":{"rendered":"<p><span id=\"urn:enhancement-aa5a9da1-017e-408e-90fd-a011413b1827\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> is a <span id=\"urn:enhancement-e11b23b4-0a98-48ce-9784-aee6a21e98f1\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/molecular-biology\">molecular biology<\/span> technique that amplifies specific DNA sequences from a sample. It has revolutionized the field by enabling rapid and efficient <span id=\"urn:enhancement-1fca60b3-dfa8-49b4-8f40-b5a3e39b16ac\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/dna-replication\">DNA amplification<\/span> and is widely used in research and industry.<\/p>\n<p><span id=\"urn:enhancement-63ca9dd7-b558-4ff4-bb04-e61611de898b\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> has advanced genetics by identifying mutations, analyzing gene <span id=\"urn:enhancement-eef703fe-ba44-4412-aafa-6725cfe0f366\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/expression\">expression<\/span>, and sequencing DNA. In forensics, <span id=\"urn:enhancement-9f86f9ba-5edf-406f-843b-0768225e4565\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> is used to match DNA from crime scenes to suspects or victims. <span id=\"urn:enhancement-92abc0bf-bf4d-414d-bf87-4a35ec3eeedd\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> is applied in medicine to diagnose genetic disorders, infectious diseases, and cancers.<\/p>\n<p><span id=\"urn:enhancement-fedb14b3-a990-4739-ae8e-3ad416339f9c\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/biotechnology\">Biotechnology<\/span> uses <span id=\"urn:enhancement-1ab3f027-1a80-4818-b689-7f57aafc50f0\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> to clone genes, produce recombinant proteins, and develop gene therapies. <span id=\"urn:enhancement-74ad983a-3303-404e-9e0c-a3c551feb732\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> is also used in environmental science to monitor <span id=\"urn:enhancement-ad46f1c9-eee7-430a-9165-b118145997cf\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/microbial\">microbial<\/span> populations, detect pollutants, and study <span id=\"urn:enhancement-5e576c6c-f1e1-4f46-a1dd-9c3a60e84e36\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/microbial\">microbial<\/span> diversity. Ongoing developments in <span id=\"urn:enhancement-0f3df7cf-0f81-4428-b335-6792e474d936\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> technology aim to improve DNA amplification, sensitivity, and accuracy. These advances are crucial for progress in the research industry, and to address new challenges in medicine, agriculture, and ecology.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-8709\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2023\/04\/10.2.jpeg\" alt=\"\" width=\"264\" height=\"307\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2023\/04\/10.2.jpeg 430w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2023\/04\/10.2-258x300.jpeg 258w\" sizes=\"(max-width: 264px) 100vw, 264px\" \/><\/p>\n<p style=\"text-align: center;\">Improvements in PCR Instrumentation<\/p>\n<p><strong><u>Multiplex <span id=\"urn:enhancement-c6881676-afec-418e-b3ee-9d4dc2bc2256\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> and High-Throughput Sequencing<\/u><\/strong><\/p>\n<ul>\n<li>Multiplex <span id=\"urn:enhancement-9a6632db-77e3-4329-ae8b-c5a48b2f1286\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> and High-Throughput Sequencing are two widely used <span id=\"urn:enhancement-b06e2585-1c2b-4aec-8cfd-197d94888f4b\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/molecular-biology\">molecular biology<\/span> techniques in genomics research.<\/li>\n<li>Multiplex <span id=\"urn:enhancement-1c418965-b9be-4a07-9f5e-32e7bfe7cb2f\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> allows the simultaneous amplification of multiple target <span id=\"urn:enhancement-c8adf0ca-147d-44e0-b00d-ade90fbd5ab3\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/dna-sequence\">DNA sequences<\/span> in a single reaction by using specific primer sets for each target sequence. The amplified products from Multiplex <span id=\"urn:enhancement-deea3d06-632a-4643-ba1e-4b1227ece233\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> can be detected using different methods like sequencing, gel electrophoresis, etc. This technique is useful in several applications like detecting mutations, genotyping, and identifying pathogens in complex samples.<\/li>\n<li>High-Throughput Sequencing enables the rapid sequencing of large quantities of DNA or RNA in parallel, using platforms like Illumina, Oxford Nanopore Technologies, and more. It is useful in applications like genome sequencing, <span id=\"urn:enhancement-5fd4bbef-13a8-4bfe-9e91-41eb88993a65\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/transcriptomics\">transcriptomics<\/span>, metagenomics, and epigenomics. High-Throughput Sequencing involves fragmenting DNA or RNA into smaller pieces and then sequencing them in parallel.<\/li>\n<li>Multiplex <span id=\"urn:enhancement-ad77baac-86ed-462e-be33-fa17ec612d65\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> and High-Throughput Sequencing are used together in several genomics research applications like characterizing <span id=\"urn:enhancement-580fa592-3e7a-4772-8132-6538c0d36cd8\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/microbial\">microbial<\/span> communities, analyzing gene <span id=\"urn:enhancement-de431f9e-b869-4003-b4da-4773dae1ca2a\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/expression\">expression<\/span> patterns, and identifying disease-causing mutations. These techniques have revolutionized genomics research by allowing faster, more efficient, and more comprehensive analysis of DNA and RNA.<\/li>\n<\/ul>\n<p>Overall, Multiplex <span id=\"urn:enhancement-13df1aae-9dc9-4f2b-b47a-5598e239d8c8\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> and High-Throughput Sequencing have significantly contributed to our understanding of genetics and the development of personalized medicine.<\/p>\n<p><strong><u>Isothermal <span id=\"urn:enhancement-5c2ef34f-8b9c-4010-b784-de5e56fc1552\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> and Digital <span id=\"urn:enhancement-7810adb3-a9c6-42ac-93f8-671a1b5d3c14\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span><\/u><\/strong><\/p>\n<p>Unlike conventional <span id=\"urn:enhancement-ae76faa5-72d6-43cd-8cb5-3f3b2bafa561\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span>, real-time <span id=\"urn:enhancement-4a633cfa-eb93-45c3-abbd-b77baa33ee5e\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> is used to quantify the amount of <span id=\"urn:enhancement-ed869b0a-11a0-40ad-a66e-caf819421fc0\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/nucleic-acid\">nucleic acid<\/span> in a given sample whereas absolute quantification requires the use of known standards to calculate the concentration for a sample, while relative quantification uses a &#8220;calibrant&#8221; to determine the fold change in the sample.<\/p>\n<p><strong>Real-time <span id=\"urn:enhancement-5efa425e-85e9-4e32-b966-5433a4ee7b38\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span><\/strong> can be used for direct measurement of DNA targets or it can be coupled with RT-<span id=\"urn:enhancement-1e3a472e-63bb-462e-8018-ccd2b71ebff6\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> to quantify RNA targets. Digital <span id=\"urn:enhancement-10236136-5f0b-470a-be71-c01c1d61d3ea\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> has only recently become widely available and provides a means to quantify targets in a sample based on direct estimation rather than by making estimates from standard curves. Many isothermal amplification methods have been developed to amplify <span id=\"urn:enhancement-a3dd2767-303d-4ee5-89d2-772dde5846f5\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/nucleic-acid\">nucleic acid<\/span> targets without the need for thermal cycler technologies<strong>.\u00a0<\/strong><\/p>\n<p><strong>Droplet Digital <span id=\"urn:enhancement-7b8de69d-9d50-42fc-8ca8-54c7bae00fa8\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> (ddPCR)<\/strong> is a promising technique for the non-<span id=\"urn:enhancement-815d048a-7991-47c8-ab41-42a7fb7f9dbd\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/invasive\">invasive<\/span> prenatal detection of fetal single-gene point mutations in maternal plasma. It offers high sensitivity, specificity, and reproducibility, allowing for reliable detection and quantification of mutant alleles. Further studies and validation are needed to fully establish its clinical utility and cost-effectiveness.<\/p>\n<p><strong><u>Challenges and Limitations in the Development of <span id=\"urn:enhancement-4204bd12-6011-49af-98cf-77e38807e7a5\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> Technology<\/u><\/strong><\/p>\n<ul>\n<li>RT-<span id=\"urn:enhancement-1302ccd4-c59e-41e8-9f07-13d132a7306a\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> tests have played a crucial role in identifying COVID-19 cases, and their accuracy has been <span id=\"urn:enhancement-ad7baf0e-62a5-4766-afb2-9ca7566d1352\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/enhanced\">enhanced<\/span> with recent developments.<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-8703\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2023\/04\/10.1-Schematic-illustration-of-the-operating-process-of-the-miniature-RT-PCR-system-a_1-1.jpeg\" alt=\"\" width=\"292\" height=\"239\" \/><\/p>\n<ul>\n<li>Nevertheless, obstacles such as the possibility of inaccurate negative outcomes, problems with gathering and shipping samples, and the requirement for ongoing refining and uniformity of testing procedures still exist.<\/li>\n<li>The continuous progress and improvement of RT-<span id=\"urn:enhancement-44ef2646-603c-4e44-bdf8-05d6da053cf2\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> testing are essential in boosting their precision and accessibility, in the battle against the pandemic.<\/li>\n<\/ul>\n<p><strong><u>Recent Innovations in <span id=\"urn:enhancement-442416ef-72da-4089-82ad-67b5ff3fd117\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> Technology<\/u><\/strong><\/p>\n<p><span id=\"urn:enhancement-251593b1-2747-4e31-a164-162019ee4216\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> technology has undergone several innovative developments in recent years, further enhancing its efficiency and applications. Some of the recent innovations in <span id=\"urn:enhancement-019ca48a-05c0-4726-9d2c-09f7f91bcc6a\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> technology are:<\/p>\n<p><span id=\"urn:enhancement-f5aba568-3e75-4b1c-8360-523d83adc569\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-Cas technology has revolutionized the field of genome editing, and\u00a0<strong><span id=\"urn:enhancement-84508ece-8206-4fc3-b8e7-c405eaaebf4c\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-Cas-assisted <span id=\"urn:enhancement-aa24c829-ea60-4a72-b86c-92bf82ddf09e\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span><\/strong>\u00a0enables more efficient and precise <span id=\"urn:enhancement-1093663a-5565-44d2-bb69-d24b76cb9459\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/gene-editing\">gene editing<\/span>. Ongoing research is expected to lead to continued advancements in <span id=\"urn:enhancement-0c0a788b-62ad-4740-8643-f81fcdacc4ba\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-based diagnostics, improving accuracy, sensitivity, and ease of use. <span id=\"urn:enhancement-6cf453b5-2fe2-4787-94d3-14b83e5fe5c2\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-based COVID-19 testing has the potential to revolutionize infectious disease detection and management, especially for next-generation point-of-care diagnostics.<\/p>\n<p><strong>Loop-mediated isothermal amplification (LAMP)\u00a0<\/strong>is a novel isothermal amplification method that amplifies DNA with high specificity and speed. It is used for the detection of infectious agents and genetic disorders.<\/p>\n<p><strong><span id=\"urn:enhancement-59575195-68b5-4928-82e1-fff961256879\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-based diagnostic tests\u00a0<\/strong>use <span id=\"urn:enhancement-39af59bc-3a46-4620-bfcb-5a7e0147e6c6\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-Cas technology to detect specific <span id=\"urn:enhancement-f95460dc-5478-4d52-b74e-6f7b444426f8\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/nucleic-acid\">nucleic acid<\/span> sequences. They are highly specific and sensitive, enabling the detection of pathogens and genetic disorders with high accuracy.<\/p>\n<p><strong><u>Final Thoughts on the Future of <span id=\"urn:enhancement-216eb601-5a14-4e76-aa59-0e0db9b06e0a\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> Technology<\/u><\/strong><\/p>\n<p><span id=\"urn:enhancement-38ce4d1e-0687-42c1-b9cf-ead8e643b4bc\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> technology has transformed the field of <span id=\"urn:enhancement-18483dce-0ec5-4bb7-869c-0e1eaabc3e2b\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/molecular-biology\">molecular biology<\/span> and has emerged as a potent tool in numerous areas. Like any other technology, <span id=\"urn:enhancement-38ec30eb-0376-4f92-bfec-3bd7a1243d86\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> is continually progressing, with several advancements anticipated in the future. One such area of progress is the miniaturization of <span id=\"urn:enhancement-e27d2149-c442-4601-aa3a-76fa917a833d\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span>, which allows <span id=\"urn:enhancement-870b4757-fb68-40d8-b7ce-f37ed982aa7c\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/dna-samples\">DNA samples<\/span> to be analyzed in lesser quantities and at higher rates. Another promising development is the use of <span id=\"urn:enhancement-eee5e547-b248-4aeb-977b-516a28736c14\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> for detecting pathogens and infectious diseases, resulting in rapid diagnosis and treatment. The creation of new diagnostic tests based on <span id=\"urn:enhancement-cc3ed0a0-7e28-4858-80b0-205d69d6eff1\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> has been particularly crucial during the ongoing COVID-19 pandemic. Furthermore, the addition of artificial intelligence (AI) and machine learning algorithms to <span id=\"urn:enhancement-e721617b-acf3-4913-a2a1-a8f15f016912\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> data analysis could assist in automating the process and improving the accuracy of outcomes.<\/p>\n<p>Overall, the future looks bright for <span id=\"urn:enhancement-85263bda-21a4-4b03-bd28-87de87e8af84\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> technology, with further advancements expected to enhance its effectiveness, speed, and precision, making it an increasingly useful tool in a diverse range of fields.<\/p>\n<p><strong>References<\/strong><\/p>\n<ol>\n<li>Hanliang Zhu et.al,\u00a0\u00a0https:\/\/www.future-science.com\/doi\/10.2144\/btn-2020-0057.<\/li>\n<li>Microfluidic Systems Integrated With a Sample Pretreatment Device for Fast <span id=\"urn:enhancement-c9317e9a-90a5-487f-b50e-5dfe18bd52a7\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/nucleic-acid\">Nucleic-Acid<\/span> Amplification May 2008, DOI:10.1109\/JMEMS.2008.916295<\/li>\n<li><span id=\"urn:enhancement-75960985-cffb-4986-9154-6c5e626b2308\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span>, Real-Time <span id=\"urn:enhancement-42c607da-0165-4d8d-834f-11552951be76\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span>, Digital <span id=\"urn:enhancement-715c3bf5-bb23-4886-85b9-9c56fa6f0cbe\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span>, and Isothermal Amplification Rachel A. Bartholomew, et.al; First published: 01 September 2015,<\/li>\n<li>Elisabetta D\u2019Aversa, et.al; Droplet Digital <span id=\"urn:enhancement-a57dfda6-1034-4d25-915a-9e170b37cb6c\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> for Non-<span id=\"urn:enhancement-963d9179-fec3-46cb-92fb-5ebcabff0f10\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/invasive\">Invasive<\/span> Prenatal Detection of Fetal Single-Gene Point Mutations in Maternal Plasma, https:\/\/doi.org\/10.3390%2Fijms23052819<\/li>\n<li>Manoucher Teymouri et.al; &#8220;Recent advances and challenges of RT-<span id=\"urn:enhancement-7d426e16-facb-4c98-9a7e-1753f9347866\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/pcr\">PCR<\/span> tests for the diagnosis of COVID-19&#8243;, Pathol Res Pract. 2021 May; 221: 153443, Published online 2021 Apr 14. DOI10.1016\/j.prp.2021.153443<\/li>\n<li>&#8220;<span id=\"urn:enhancement-1d80bd20-fc16-477d-8924-f4a6e1aa77d6\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-Based COVID -19 Testing: Toward Next \u2013Generation Point\u2013of\u2013Care Diagnostics&#8221;, Front. Cell. Infect. Microbiol., 30 April 2021, Sec. Clinical <span id=\"urn:enhancement-5c3ecb59-7f43-4f0f-92c0-f9e516a5413a\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/microbiology\">Microbiology<\/span>, Volume 11 \u2013 2021, https:\/\/doi.org\/10.3389\/fcimb.2021.663949<\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>PCR is a molecular biology technique that amplifies specific DNA sequences from a sample. It has revolutionized the field by enabling rapid and efficient DNA amplification and is widely used in research and industry. PCR has advanced genetics by identifying mutations, analyzing gene expression, and sequencing DNA. In forensics, PCR is used to match DNA [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-8701","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/pages\/8701","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/comments?post=8701"}],"version-history":[{"count":0,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/pages\/8701\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/media?parent=8701"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}