{"id":9770,"date":"2026-09-11T08:09:48","date_gmt":"2026-09-11T08:09:48","guid":{"rendered":"https:\/\/www.mybiosource.com\/learn\/?page_id=9770"},"modified":"2026-09-11T08:14:29","modified_gmt":"2026-09-11T08:14:29","slug":"what-is-immunoprecipitation","status":"publish","type":"page","link":"https:\/\/www.mybiosource.com\/learn\/what-is-immunoprecipitation\/","title":{"rendered":"What Is Immunoprecipitation? Principles, Methods, and Applications"},"content":{"rendered":"\n<style>.mbs-page, .mbs-page * { margin: 0; padding: 0; box-sizing: border-box; } html { scroll-behavior: smooth; } .mbs-page { font-family: -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, sans-serif; line-height: 1.65; color: #1f2937; background: #fff; } .mbs-page img { max-width: 100%; } .mbs-page .wrap { max-width: 1140px; margin: 0 auto; padding: 0 1.25rem; } .mbs-page .nav { position: sticky; top: 0; z-index: 50; background: rgba(255,255,255,0.92); backdrop-filter: blur(8px); border-bottom: 1px solid #e5e7eb; } .mbs-page .nav .wrap { height: 4rem; display: flex; align-items: center; justify-content: space-between; gap: 1rem; } .mbs-page .nav-links { display: none; gap: 0.25rem; } .mbs-page .nav-links a { color: #374151; text-decoration: none; font-size: 0.875rem; font-weight: 500; padding: 0.45rem 0.9rem; border-radius: 999px; } .mbs-page .nav-links a:hover { background: #eff6ff; color: #1d4ed8; } .mbs-page .nav-btn { background: none; 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text-decoration: none; } .mbs-page .site-footer a:hover { color: #60a5fa; } .mbs-page .site-footer .bottom { max-width: 1200px; margin: 2.5rem auto 0; padding: 1.5rem 1.25rem 0; border-top: 1px solid rgba(255,255,255,0.12); font-size: 0.8125rem; color: #6b7280; } @media (min-width: 768px) {.mbs-page .site-footer .wrap { grid-template-columns: 2fr 1fr 1fr; } } .mbs-page .eyebrow { margin-bottom: 1rem; } .mbs-page .strip .n { font-size: 2rem; font-weight: 800; color: #bfdbfe; line-height: 1; margin-bottom: 0.5rem; } .mbs-page .flow-step .step { font-size: 0.75rem; letter-spacing: 0.12em; text-transform: uppercase; color: #2563eb; font-weight: 700; margin-bottom: 0.5rem; } .mbs-page .flow-step a, .mbs-page .tl-item a, .mbs-page .faq-item a, .mbs-page .cta p a, .mbs-page .section-head a { color: #1d4ed8; text-decoration: none; font-weight: 500; } .mbs-page .tile p + p { margin-top: 0.75rem; } .mbs-page .pick p + p { margin-top: 0.75rem; } .mbs-page .prose ul { margin: 0 0 1rem 1.25rem; max-width: 46rem; } .mbs-page .prose li { font-size: 1.0625rem; color: #374151; margin-bottom: 0.5rem; } .mbs-page .related p { display: contents; } .mbs-page .related a { flex-direction: row; } .mbs-page .related a .txt { flex: 1; display: block; } .mbs-page .related a .txt span { display: block; } .mbs-page .igtable td.hd { font-weight: 700; color: #0f172a; white-space: nowrap; } .mbs-page h1, .mbs-page h2, .mbs-page h3, .mbs-page h4 { padding: 0; } .mbs-page p { padding: 0; } .mbs-page a { text-decoration: none; } .mbs-page .cta h2 { color: #fff; line-height: 1.2; } .mbs-page .section-head h2, .mbs-page .tl-item h3, .mbs-page .flow-step h3, .mbs-page .pick h3, .mbs-page .tile h3, .mbs-page .faq-item h3 { line-height: 1.2; }<\/style>\n<p><script type=\"application\/ld+json\"> { \"@context\": \"https:\/\/schema.org\", \"@type\": \"FAQPage\", \"mainEntity\": [ { \"@type\": \"Question\", \"name\": \"Can I use the same antibody for immunoprecipitation and western blot detection?\", \"acceptedAnswer\": { \"@type\": \"Answer\", \"text\": \"Sometimes, but not automatically. IP requires an antibody to recognize its epitope under native or near-native conditions, whereas western blot detection often works with a denatured, linearized protein on a membrane. An antibody validated for western blot alone may bind a conformational epitope that no longer exists once the protein is denatured for IP. Check the antibody's datasheet for IP-specific validation before assuming cross-application performance.\" } }, { \"@type\": \"Question\", \"name\": \"Should I incubate the antibody with beads first or with the lysate first?\", \"acceptedAnswer\": { \"@type\": \"Answer\", \"text\": \"Both orders work, and the choice depends on what matters most for a given experiment. Adding the antibody directly to the lysate first, then adding beads afterward, often gives a higher capture yield because the antibody has unrestricted access to its target. Pre-binding the antibody to beads before adding lysate reduces the amount of nonspecific protein the beads pick up on their own, which can produce a cleaner result when background is the bigger concern than yield.\" } }, { \"@type\": \"Question\", \"name\": \"What is the difference between immunoprecipitation and co-immunoprecipitation?\", \"acceptedAnswer\": { \"@type\": \"Answer\", \"text\": \"Standard immunoprecipitation isolates a single target protein and typically uses a lysis buffer that doesn't need to preserve anything beyond that protein's structure. Co-immunoprecipitation isolates the target protein along with any binding partners still attached to it, so the lysis conditions must remain gentle enough to preserve those protein-protein interactions throughout the capture and wash process.\" } } ] } <\/script><\/p>\n<div class=\"mbs-page\">\n<header class=\"nav\">\n<div class=\"wrap\">\n<div class=\"logo\"><a href=\"https:\/\/www.mybiosource.com\/\"><img decoding=\"async\" src=\"https:\/\/cdn.mybiosource.com\/antibody-protein-elisa-kit.png\" alt=\"MyBioSource\" style=\"height:2rem;display:block;\" \/><\/a><\/div>\n<nav class=\"nav-links\"><a href=\"#the-principle-behind-immunoprecipitation\">Principle<\/a><a href=\"#key-variations-on-the-ip-protocol\">Variations<\/a><a href=\"#practical-considerations\">Protocol<\/a><a href=\"#troubleshooting-common-ip-problems\">Troubleshooting<\/a><a href=\"#from-ip-to-downstream-analysis\">Downstream<\/a><a href=\"#faqs\">FAQ<\/a><\/nav>\n<div class=\"nav-btn-wrap\"><button class=\"nav-btn\" type=\"button\" onclick=\"document.getElementById('nav-mobile').classList.toggle('open')\" aria-label=\"Menu\">&#9776;<\/button><\/div>\n<\/div>\n<div class=\"nav-mobile\" id=\"nav-mobile\"><a href=\"#the-principle-behind-immunoprecipitation\">Principle<\/a><a href=\"#key-variations-on-the-ip-protocol\">Variations<\/a><a href=\"#practical-considerations\">Protocol<\/a><a href=\"#troubleshooting-common-ip-problems\">Troubleshooting<\/a><a href=\"#from-ip-to-downstream-analysis\">Downstream<\/a><a href=\"#faqs\">FAQ<\/a><\/div>\n<\/header>\n<section class=\"hero\">\n<div class=\"wrap\">\n<div>\n<p class=\"eyebrow\">Protein Methods Explained<\/p>\n<h1>What Is Immunoprecipitation? Principles, Methods, and Applications<\/h1>\n<p class=\"lead\">Immunoprecipitation (IP) is a technique that uses an antibody to isolate a specific protein from a complex biological sample, such as a cell or tissue lysate, so it can be studied on its own. The antibody binds its target antigen in solution, and a solid-phase support, either agarose or magnetic beads coated with Protein A or Protein G, captures the antibody-antigen complex, allowing researchers to wash it free of everything else in the sample. What&#8217;s left behind is a concentrated, purified version of the protein a researcher wants to analyze, ready for western blotting, mass spectrometry, or further biochemical characterization.<\/p>\n<div class=\"meta\"><span>MyBioSource Learning Library<\/span><span>&middot;<\/span><span>Antibodies &amp; Protein Analysis<\/span><\/div>\n<\/div>\n<div class=\"hero-img\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2026\/09\/what-is-immunoprecipitation.jpg\" alt=\"Six clear microcentrifuge tubes with white caps arranged in a metal sample rack, containing clear solution with visible material or precipitate at the bottom of each tube\" width=\"1200\" height=\"800\" \/><\/div>\n<\/div>\n<\/section>\n<section class=\"strip\" aria-label=\"Key takeaways\">\n<div class=\"wrap\">\n<div class=\"item\">\n<div class=\"n\">01<\/div>\n<p>Immunoprecipitation captures a target protein from a complex sample by binding it to an antibody attached to agarose or magnetic beads, and then separating the antibody-antigen complex from the rest of the lysate.<\/p>\n<\/div>\n<div class=\"item\">\n<div class=\"n\">02<\/div>\n<p>Co-immunoprecipitation (Co-IP) preserves native protein complexes to study protein-protein interactions, while ChIP captures protein-DNA binding and RIP captures protein-RNA binding.<\/p>\n<\/div>\n<div class=\"item\">\n<div class=\"n\">03<\/div>\n<p>The choice of lysis buffer determines the outcome: nondenaturing buffers preserve protein complexes for Co-IP, while RIPA buffer works well when a single target protein is the goal.<\/p>\n<\/div>\n<div class=\"item\">\n<div class=\"n\">04<\/div>\n<p>Pre-clearing a lysate with unconjugated beads before adding the antibody reduces nonspecific binding that appears as background on a western blot.<\/p>\n<\/div>\n<\/div>\n<\/section>\n<section class=\"section \">\n<div class=\"wrap\">\n<div class=\"section-head\">\n<h2 id=\"the-principle-behind-immunoprecipitation\">The Principle Behind Immunoprecipitation<\/h2>\n<\/div>\n<div class=\"prose columns\">\n<p>So how does immunoprecipitation work at the molecular level? The immunoprecipitation principle depends on the same lock-and-key specificity that makes antibodies useful reagents throughout the lab. An antibody&#8217;s paratope binds a matching epitope on the target antigen with enough affinity to hold the two together through multiple wash steps, but not so tightly that elution becomes impossible later.<\/p>\n<p>That antibody-antigen pair still needs a way to drop out of solution, which is where the &#8220;precipitation&#8221; half of the name comes from. Protein A or Protein G, immobilized on agarose or magnetic beads, binds the constant region of the antibody rather than its antigen-binding site, so the whole complex, antibody plus captured antigen, gets pulled down together when researchers spin down the beads or pull them out with a magnet. Rinse the beads, elute the bound protein, and the target is isolated from thousands of other proteins that were present in the same lysate a few steps earlier.<\/p>\n<\/div>\n<\/div>\n<\/section>\n<section class=\"section section-alt\">\n<div class=\"wrap\">\n<div class=\"section-head\">\n<h2 id=\"key-variations-on-the-ip-protocol\">Key Variations on the IP Protocol<\/h2>\n<p>Standard IP isolates one protein. Three related methods extend the same antibody-capture principle to answer different biological questions.<\/p>\n<\/div>\n<div class=\"picks\">\n<div class=\"pick\">\n<h3>Co-IP<\/h3>\n<p><strong>Co-immunoprecipitation (Co-IP)<\/strong> captures a target protein along with any binding partners still attached to it, which makes it the go-to method for confirming protein-protein interactions in a native cellular context. Because the interaction itself is the point, Co-IP depends on keeping those complexes intact through lysis and washing.<\/p>\n<\/div>\n<div class=\"pick\" style=\"border-top-color:#16a34a;\">\n<h3>ChIP<\/h3>\n<p><strong>Chromatin immunoprecipitation (<\/strong><strong>ChIP<\/strong><strong>)<\/strong> maps where a protein binds along genomic DNA. Researchers typically crosslink cells with formaldehyde to fix protein-DNA contacts, then shear the chromatin by sonication. As a result, an antibody against the protein of interest pulls down the associated DNA fragments for qPCR or sequencing.<\/p>\n<\/div>\n<div class=\"pick\" style=\"border-top-color:#9333ea;\">\n<h3>RIP<\/h3>\n<p><strong>RNA immunoprecipitation (RIP) <\/strong>follows a similar logic for protein-RNA interactions, capturing an RNA-binding protein together with its bound transcripts to study post-transcriptional regulation.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<section class=\"section \">\n<div class=\"wrap\">\n<div class=\"section-head\">\n<h2 id=\"practical-considerations\">Practical Considerations for a Successful IP Protocol<\/h2>\n<p>Four decisions shape whether an IP protocol delivers a clean, specific pulldown.<\/p>\n<\/div>\n<div class=\"timeline\">\n<div class=\"tl-item\" data-n=\"1\">\n<h3>Lysis Buffer Selection<\/h3>\n<p>Lysis buffer selection is the decision that determines whether the rest of the protocol succeeds. A nondenaturing buffer containing mild detergents such as NP-40 or Triton X-100 keeps protein complexes folded and associated, which is required for Co-IP. RIPA buffer, which includes stronger ionic detergents, works well for standard single-protein IP where preserving a binding partner isn&#8217;t the goal, but it can strip away the interactions Co-IP depends on.<\/p>\n<\/div>\n<div class=\"tl-item\" data-n=\"2\">\n<h3>Pre-Clearing the Lysate<\/h3>\n<p>Pre-clearing the lysate with beads alone, before adding the antibody, removes proteins that stick to the bead matrix or to Protein A\/G, regardless of antibody specificity. Skipping this step is one of the most common sources of background bands on a downstream western blot.<\/p>\n<\/div>\n<div class=\"tl-item\" data-n=\"3\">\n<h3>Bead Format<\/h3>\n<p>Bead format matters for speed and yield. Agarose beads offer a larger binding surface and are often less expensive at scale, while magnetic beads allow faster washes without centrifugation and tend to produce cleaner pulldowns with lower sample loss, which matters when the starting material is limited.<\/p>\n<\/div>\n<div class=\"tl-item\" data-n=\"4\">\n<h3>Choosing the Right Antibody<\/h3>\n<p>Choosing the right antibody matters as much as the buffer. Our <a href=\"https:\/\/www.mybiosource.com\/monoclonal\">monoclonal antibodies<\/a> offer consistent, single-epitope binding that&#8217;s useful when lot-to-lot reproducibility is critical. Our <a href=\"https:\/\/www.mybiosource.com\/polyclonal\">polyclonal antibodies<\/a> recognize multiple epitopes on the same target and can improve capture efficiency for low-abundance proteins. For detection after elution, a validated <a href=\"https:\/\/www.mybiosource.com\/secondary\">secondary antibody<\/a> still serves the same amplification role as in western blotting or ELISA.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<section class=\"section section-dark\">\n<div class=\"wrap\">\n<div class=\"section-head\">\n<h2 id=\"troubleshooting-common-ip-problems\">Troubleshooting Common IP Problems<\/h2>\n<\/div>\n<div class=\"tools\">\n<div class=\"img\"><img decoding=\"async\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2026\/09\/ip-protocol.jpg\" alt=\"Hands in blue gloves holding a 96-well plate displaying a color gradient of samples from clear green to purple and deep blue during high-throughput immunoprecipitation or protein assay\" width=\"1200\" height=\"480\" loading=\"lazy\" \/><\/div>\n<div class=\"tile featured span2\">\n<h3>Weak Signal or High Background<\/h3>\n<p>A weak or absent signal usually traces back to one of three causes: an antibody that isn&#8217;t validated for IP applications specifically, insufficient antibody concentration, or epitope masking caused by the lysis buffer. Excess background typically means the pre-clear step was skipped, wash stringency was too low, or the bead-to-lysate ratio left too much unbound protein in the mix.<\/p>\n<\/div>\n<div class=\"tile\">\n<h3>Run an Isotype Control<\/h3>\n<p>Running a control immunoprecipitation with an isotype-matched, nonspecific antibody in parallel isolates whether a band is a real signal or an artifact of the capture chemistry.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<section class=\"section section-alt\">\n<div class=\"wrap\">\n<div class=\"section-head\">\n<h2 id=\"from-ip-to-downstream-analysis\">From Immunoprecipitation to Downstream Analysis<\/h2>\n<\/div>\n<div class=\"prose columns\">\n<p>An immunoprecipitation experiment produces a purified protein sample, not a finished result on its own. Western blotting remains the most common next step, confirming the identity and relative abundance of the captured protein; see our <a href=\"https:\/\/www.mybiosource.com\/learn\/westernblotting\/\">western blotting guide<\/a> for details on transfer conditions and antibody dilution.<\/p>\n<p>Mass spectrometry identifies unknown binding partners pulled down in a Co-IP, turning a single pulldown into a map of an entire protein complex. Some labs pair IP with <a href=\"https:\/\/www.mybiosource.com\/learn\/elisa-testing\/\">ELISA-based quantification<\/a> to measure the amount of target protein recovered. Projects that need a defined positive control alongside the pulldown often turn to <a href=\"https:\/\/www.mybiosource.com\/rec-purified-protein\">recombinant purified protein<\/a> standards to confirm assay performance. For the full bench protocol, our <a href=\"https:\/\/www.mybiosource.com\/learn\/testing-procedures\/immunoprecipitation\/\">immunoprecipitation methods<\/a> page walks through the step-by-step procedure in more depth.<\/p>\n<\/div>\n<\/div>\n<\/section>\n<section class=\"cta\">\n<div class=\"wrap\">\n<div>\n<h2>Find the Right Antibody for Your Immunoprecipitation Protocol<\/h2>\n<p>We supply monoclonal and polyclonal antibodies validated for IP, Co-IP, ChIP, and RIP applications, along with secondary antibodies and purified protein standards to complete a pulldown workflow. Browse our catalog to find a reagent matched to your target, or reach out to our team if your project needs a custom antibody build.<\/p>\n<\/div>\n<div class=\"btns\"><a class=\"primary\" href=\"https:\/\/www.mybiosource.com\/contact-us\">Contact Us<\/a><a class=\"ghost\" href=\"https:\/\/www.mybiosource.com\/monoclonal\" target=\"_blank\" rel=\"noopener noreferrer\">Browse Monoclonal Antibodies<\/a><a class=\"ghost\" href=\"https:\/\/www.mybiosource.com\/polyclonal\" target=\"_blank\" rel=\"noopener noreferrer\">Browse Polyclonal Antibodies<\/a><\/div>\n<\/div>\n<\/section>\n<section class=\"section \">\n<div class=\"wrap\">\n<div class=\"section-head\">\n<h2 id=\"faqs\">Frequently Asked Questions About Immunoprecipitation<\/h2>\n<\/div>\n<div class=\"faq-grid\">\n<div class=\"faq-item\">\n<h3><span>Q<\/span>Can I use the same antibody for immunoprecipitation and western blot detection?<\/h3>\n<p>Sometimes, but not automatically. IP requires an antibody to recognize its epitope under native or near-native conditions, whereas western blot detection often works with a denatured, linearized protein on a membrane. An antibody validated for western blot alone may bind a conformational epitope that no longer exists once the protein is denatured for IP. Check the antibody&#8217;s datasheet for IP-specific validation before assuming cross-application performance.<\/p>\n<\/div>\n<div class=\"faq-item\">\n<h3><span>Q<\/span>Should I incubate the antibody with beads first or with the lysate first?<\/h3>\n<p>Both orders work, and the choice depends on what matters most for a given experiment. Adding the antibody directly to the lysate first, then adding beads afterward, often gives a higher capture yield because the antibody has unrestricted access to its target. Pre-binding the antibody to beads before adding lysate reduces the amount of nonspecific protein the beads pick up on their own, which can produce a cleaner result when background is the bigger concern than yield.<\/p>\n<\/div>\n<div class=\"faq-item\">\n<h3><span>Q<\/span>What is the difference between immunoprecipitation and co-immunoprecipitation?<\/h3>\n<p>Standard immunoprecipitation isolates a single target protein and typically uses a lysis buffer that doesn&#8217;t need to preserve anything beyond that protein&#8217;s structure. Co-immunoprecipitation isolates the target protein along with any binding partners still attached to it, so the lysis conditions must remain gentle enough to preserve those protein-protein interactions throughout the capture and wash process.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n<section class=\"section section-alt\" style=\"padding-top:3rem;padding-bottom:3rem;\">\n<div class=\"wrap\">\n<div class=\"section-head\" style=\"margin-bottom:1.25rem;\">\n<h2 style=\"font-size:1.25rem;\">Related Articles<\/h2>\n<\/div>\n<div class=\"related\"><a href=\"https:\/\/www.mybiosource.com\/learn\/what-is-dna\/\"><span class=\"txt\"><b>What Is DNA? Everything You Need To Know<\/b><span>Get an in-depth look at DNA and its role in research.<\/span><\/span><i>&rarr;<\/i><\/a><a href=\"https:\/\/www.mybiosource.com\/learn\/southern-blotting\/\"><span class=\"txt\"><b>Southern Blotting<\/b><span>Learn how Southern blotting helps to detect specific sequences of DNA in DNA samples.<\/span><\/span><i>&rarr;<\/i><\/a><a href=\"https:\/\/www.mybiosource.com\/learn\/branched-dna-bdna-technology\/\"><span class=\"txt\"><b>Branched DNA (bDNA) Technology<\/b><span>Discover how this unique and powerful tool provides reliable quantification of nucleic acid molecules.<\/span><\/span><i>&rarr;<\/i><\/a><\/div>\n<\/div>\n<\/section>\n<footer class=\"site-footer\">\n<div class=\"wrap\">\n<div>\n<div class=\"logo\"><img decoding=\"async\" src=\"https:\/\/cdn.mybiosource.com\/antibody-protein-elisa-kit.png\" alt=\"MyBioSource\" style=\"height:2rem;margin-bottom:1rem;\" \/><\/div>\n<p>Your trusted partner in life science research. Providing high-quality ELISA kits, antibodies, and research tools to scientists worldwide.<\/p>\n<\/div>\n<div>\n<h4>Products &amp; Services<\/h4>\n<ul>\n<li><a href=\"https:\/\/www.mybiosource.com\/elisa-kits\" target=\"_blank\" rel=\"noopener noreferrer\">ELISA Kits<\/a><\/li>\n<li><a href=\"https:\/\/www.mybiosource.com\/antibodies\" target=\"_blank\" rel=\"noopener noreferrer\">Antibodies<\/a><\/li>\n<li><a href=\"https:\/\/www.mybiosource.com\/protein\" target=\"_blank\" rel=\"noopener noreferrer\">Proteins &amp; Peptides<\/a><\/li>\n<li><a href=\"https:\/\/www.mybiosource.com\/assay-kits\" target=\"_blank\" rel=\"noopener noreferrer\">Assay Kits<\/a><\/li>\n<\/ul>\n<\/div>\n<div>\n<h4>Resources<\/h4>\n<ul>\n<li><a href=\"https:\/\/www.mybiosource.com\/learn\/\">Learning Library<\/a><\/li>\n<li><a href=\"https:\/\/www.mybiosource.com\/learn\/nucleotide-vs-nucleoside\/\">Nucleotide vs. Nucleoside<\/a><\/li>\n<li><a href=\"https:\/\/www.mybiosource.com\/learn\/understanding-the-building-blocks-of-dna\/\">Building Blocks of DNA<\/a><\/li>\n<li><a href=\"https:\/\/www.mybiosource.com\/learn\/what-do-antibodies-do\/\">What Do Antibodies Do?<\/a><\/li>\n<\/ul>\n<\/div>\n<\/div>\n<div class=\"bottom\">&copy; 2026 MyBioSource. All rights reserved. | Educational Resource<\/div>\n<\/footer>\n<p><script>document.querySelectorAll(\".nav-mobile a\").forEach(function(a){a.addEventListener(\"click\",function(){document.getElementById(\"nav-mobile\").classList.remove(\"open\");});});<\/script><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Learn what immunoprecipitation is, how the antibody-antigen principle works, and how to build an IP protocol for Co-IP, ChIP, and RIP applications.<\/p>\n","protected":false},"author":16,"featured_media":9766,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-template-blank.php","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-9770","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/pages\/9770","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/comments?post=9770"}],"version-history":[{"count":3,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/pages\/9770\/revisions"}],"predecessor-version":[{"id":9779,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/pages\/9770\/revisions\/9779"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/media\/9766"}],"wp:attachment":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/media?parent=9770"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}