{"id":4873,"date":"2021-03-10T10:57:17","date_gmt":"2021-03-10T10:57:17","guid":{"rendered":"https:\/\/www.mybiosource.com\/learn\/?p=4873"},"modified":"2023-03-10T14:07:19","modified_gmt":"2023-03-10T14:07:19","slug":"guide-to-understanding-crispr-gene-editing","status":"publish","type":"post","link":"https:\/\/www.mybiosource.com\/learn\/guide-understanding-crispr-gene-editing\/","title":{"rendered":"A Complete Guide To Understanding CRISPR &#038; Gene Editing"},"content":{"rendered":"\r\n<table style=\"height: 997px; background-color: #ebebe6; width: 465px;\" width=\"465\">\r\n<tbody>\r\n<tr>\r\n<td style=\"width: 455.889px;\">\r\n<h2 style=\"padding-left: 80px;\"><strong><u>Table of Contents<\/u><\/strong><\/h2>\r\n<ol>\r\n<li><a href=\"#1\"><strong>Introduction<\/strong><\/a><\/li>\r\n<li><a href=\"#2\"><strong>History of gene editing<\/strong><\/a>\r\n<ul>\r\n<li>Pre-1960s with the discovery of double helix to producing DNA in a test tube<\/li>\r\n<li>1960s \u2013 new developments concerning the structure and function of prokaryotic with the progress of viral genetic material<\/li>\r\n<li>1970s \u2013 Witness the first Chimeric recombinant DNA cloning SV40 molecule into plasmid DNA<\/li>\r\n<li>1980s \u2013 The era using genetic engineering to solve issues using human DNA<\/li>\r\n<li>1990s \u2013 The birth of Genome project<\/li>\r\n<li>2000s \u2013 Genetic engineering helps understand and cure genetic diseases<\/li>\r\n<li>2010s \u2013 Sees the invention of CRISPR and the first approval of genome editing humans<\/li>\r\n<li>Present \u2013 2020 was a huge year for CRISPR<\/li>\r\n<\/ul>\r\n<\/li>\r\n<li><a href=\"#3\"><strong>CRISPR<\/strong><\/a>\r\n<ul>\r\n<li>What is CRISPR?<\/li>\r\n<li>Discovery of CRISPR<\/li>\r\n<\/ul>\r\n<\/li>\r\n<li><a href=\"#4\"><strong>What is CAS9?<\/strong><\/a>\r\n<ul>\r\n<li>When was it developed?<\/li>\r\n<li>What is the goal of CRISPR-CAS9 technology?<\/li>\r\n<\/ul>\r\n<\/li>\r\n<li><a href=\"#5\"><strong>How CRISPR gene editing work<\/strong><\/a>\r\n<ul>\r\n<li>Selecting CRISPR and gRNA<\/li>\r\n<li>Constructing the gRNA or sgRNA by synthesis and cloning<\/li>\r\n<li>Real work sickle cell experiments<\/li>\r\n<\/ul>\r\n<\/li>\r\n<li><a href=\"#6\"><strong>What is CRISPR used for?<\/strong><\/a>\r\n<ul>\r\n<li>Using CRISPR for genome editing<\/li>\r\n<li>Using CRISPR libraries for screening<\/li>\r\n<\/ul>\r\n<\/li>\r\n<li><a href=\"#7\"><strong>Benefits of CRISPR<\/strong><\/a>\r\n<ul>\r\n<li>Treating Alzheimer disease<\/li>\r\n<li>Treating HIV<\/li>\r\n<li>Developing new drugs and on cancer treatments<\/li>\r\n<li>Reduce our need for plastic<\/li>\r\n<\/ul>\r\n<\/li>\r\n<li><a href=\"#8\"><strong>Conclusion<\/strong><\/a><\/li>\r\n<\/ol>\r\n<p>&nbsp;<\/p>\r\n<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n\r\n\r\n\r\n<div class=\"wp-block-image\">\r\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1000\" height=\"401\" class=\"wp-image-4876\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/1.Introduction-Briefly-introduce-what-gene-editing.png\" alt=\"1.Introduction - Briefly introduce what gene editing\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/1.Introduction-Briefly-introduce-what-gene-editing.png 1000w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/1.Introduction-Briefly-introduce-what-gene-editing-300x120.png 300w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/1.Introduction-Briefly-introduce-what-gene-editing-768x308.png 768w\" sizes=\"(max-width: 1000px) 100vw, 1000px\" \/><\/figure>\r\n<\/div>\r\n\r\n\r\n\r\n<p id=\"1\">The history of <span id=\"urn:enhancement-77a7ae82-9a26-4dff-b5f3-47e05cbdd385\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/gene_therapy\">gene editing<\/span> may not be a long one, but it is absolutely fascinating. In less than 80 years humanity has gone from understanding next to something about our DNA to being able to selectively edit and combine genes. We can clone animals and are on our way to being able to eradicate hereditary health conditions.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>One of the most exciting developments in this field in the last decade has been the invention of <span id=\"urn:enhancement-17e4ef13-9ac7-4f4a-b061-d249fd57864f\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-CAS9 tools. These tools harness proteins to accurately remove and replace problematic genes. These systems are more accurate than anything that has come before it has cut the time and costs involved in these experiments drastically.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Today we&#8217;ll be talking you through who the <span id=\"urn:enhancement-ca186f63-ff09-4687-aad5-ca1e68b45c1b\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-CAS9 system works, how it was discovered, and what it could do for us in the future. We&#8217;ll also be taking a deep dive into the history of <span id=\"urn:enhancement-167705ea-e94d-41ce-a3dd-30ad93c19981\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/gene_therapy\">gene editing<\/span>. Studying the past will help you to understand how fast science has come in the last 80 years, and how revolutionary tools like <span id=\"urn:enhancement-4ea8d965-2db0-4ba2-a4ab-1c69b7afc0e3\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> could be.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p id=\"2\">History of gene editing\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1000\" height=\"401\" class=\"wp-image-4877\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/2.History-of-gene-editing.png\" alt=\"2.History of gene editing\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/2.History-of-gene-editing.png 1000w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/2.History-of-gene-editing-300x120.png 300w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/2.History-of-gene-editing-768x308.png 768w\" sizes=\"(max-width: 1000px) 100vw, 1000px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>The only logical place to start this guide is by looking at the history of <span id=\"urn:enhancement-cbe57bfc-0962-465f-8e5d-9c382335fb14\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/gene_therapy\">gene editing<\/span>. This is a rich history full of amazing men and women pushing science to its very limits in order to help humanity. Their studies have changed the way we understand what it means to be human, the way we treat diseases, and even the way we eat.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>We&#8217;re going to look at some of the most important moments in the history of <span id=\"urn:enhancement-cc937d07-67d9-470c-8889-21902ee40336\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/gene_therapy\">gene editing<\/span> that will help us understand why the industry looks the way it does today.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>Pre-1960s<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignright size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4884\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/1.Pre-1960s.png\" alt=\"1.Pre-1960s\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/1.Pre-1960s.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/1.Pre-1960s-300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>The history of <span id=\"urn:enhancement-bdfedc6f-9b8a-46bc-8284-8859d6ba221f\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/gene_therapy\">gene editing<\/span> really kicks off in the 1960s. However, before this point, there were two discoveries that made all of this possible. That is where we shall begin.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1953 &#8211; A team of scientists discovers the Double Helix\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>Thanks to the <a href=\"https:\/\/io9.gizmodo.com\/the-unsung-hero-who-discovered-the-double-helix-5761388\">pioneering work of Rosalind Franklin<\/a>; James Watson and Francis Crick discovered the <span id=\"urn:enhancement-16747ea2-72a9-4e24-8fcd-2c7c61ae228b\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/double-helix\">double helix<\/span> structure of our DNA.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Franklin had been producing x-rays of DNA at the time, her work led to our modern-day understanding of how our DNA works.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p><span id=\"urn:enhancement-d554b402-8db8-4a4f-bef4-65704ce0d526\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/watson-and-crick\">Watson and Crick<\/span> noted the interlocking, ladder-like structure of our curving DNA. This discovery laid the foundation for everything to come.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1958 &#8211; Arthur Kornberg produces DNA in a test tube, a world first\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>Around the same time as the <span id=\"urn:enhancement-bffdaba5-ed30-4643-ac60-9afb75739146\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/double-helix\">double helix<\/span> was discovered, it became possible to synthesize all five <span id=\"urn:enhancement-9d38e17c-dd63-46d0-a3e2-a899a5396fb3\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/nucleotides\">nucleotides<\/span>. This was when Arthur Kornberg turned his attention to the rest of the <span id=\"urn:enhancement-80048251-4460-405a-94d2-0b63cec92995\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/dna-structure\">DNA structure<\/span>. Less than a year after learning how to <span id=\"urn:enhancement-40178e5d-76b8-4218-8264-5421554a61c9\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/polymerase\">polymerase<\/span> DNA from <span id=\"urn:enhancement-61145ae7-bdaf-4823-b8a7-747a3eba8074\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/bacterial\">bacteria<\/span> Kornberg had produced the first set of <em>in vitro <\/em>DNA.\u00a0\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>This work won Kornberg <a href=\"https:\/\/www.nobelprize.org\/prizes\/chemistry\/2006\/kornberg\/facts\/\">a Nobel prize<\/a> and many other career accolades.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>1960s<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<p>Welcome to the 1960s, gene related research is just coming into fashion in Silicon Valley. New developments are being made in every direction including new discoveries concerning the structure and function of prokaryotic. Progress is even being made in the world of viral genetic material.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1962 &#8211; Green Fluorescent Protein (from Jellyfish) enables scientists to study cellular processes that were previously invisible to them\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>The <em>Aequorea Victoria <\/em>jellyfish provided scientists with <span id=\"urn:enhancement-95e494ac-7a9a-469e-8ad8-4f960eaa1ae9\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/gfp\">GFP<\/span> (<span id=\"urn:enhancement-a3082791-3311-4a66-9c97-de22d0709366\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/gfp\">green fluorescent protein<\/span>), this allowed scientists to study processes they could not previously see.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>When exposed to long wave blue light <span id=\"urn:enhancement-2f1f91ed-a9a5-453b-8e1e-001b812db536\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/gfp\">GFP<\/span> glows green. This protein was able to reveal undiscovered chemical processes that lead to Osamu Shimomura, Martin Chalfie and Roger Tsien <a href=\"https:\/\/www.nobelprize.org\/prizes\/medicine\/2008\/summary\/\">winning a Nobel prize<\/a> for their contributions to chemistry.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1967 &#8211; Scientists perfect DNA ligation links forming recombinant DNA for the first time\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>Thanks to the work done by <a href=\"https:\/\/www.jbc.org\/article\/S0021-9258(19)82019-7\/fulltext\">Gellert, Lehman, Richardson, and Hurwitz <\/a>laboratories in the early 60s, this milestone of <span id=\"urn:enhancement-cd93f15c-eb81-4394-aad4-047f2d0bf721\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/molecular-biology\">molecular biology<\/span> was reached. They discovered a way of making DNA form of a <span id=\"urn:enhancement-5818edf0-e544-4849-8c4c-1db7078df1bb\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/phosphodiester-bond\">phosphodiester bond<\/span> which allowed the parts of the helix to join together.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>This was the work that began the process of gene splicing.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1968 &#8211; Scientists discover restriction enzymes\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>One day Weren Arber noticed that a group of <span id=\"urn:enhancement-cb4d38d7-6b5b-488b-98de-1ec0284984b4\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/bacterial\">bacteria<\/span> he was studying had a fascinating method of protecting themselves against bacteriophage infection. The <span id=\"urn:enhancement-68a855a3-d2d9-4296-9f28-b68c4ff73895\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/bacterial\">bacteria<\/span> were cutting the bacteriophage off themselves using an unknown chemical. Arber began to research how the chemical knew the difference between the <span id=\"urn:enhancement-06f172bf-c057-4670-9f4c-b8d5d38dbe1d\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/bacterial\">bacteria<\/span> and the infection.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>He believed that two types of chemicals when the infection began &#8211; a &#8216;modification&#8217; enzyme that could tell what was an infection and what was part of the host, and a &#8216;restriction&#8217; enzyme that cut away the infection.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Later when studying e.coli <a href=\"https:\/\/www.nature.com\/scitable\/spotlight\/restriction-enzymes-18458113\/\">he was able to prove his theory correct.\u00a0<\/a><\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>1970s<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignleft size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4885\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/2.1970s.png\" alt=\"2.1970s\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/2.1970s.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/2.1970s-300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>After the explosion of DNA-based research in the 1960s, the 1970s continued to see vast amounts of progress being made.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Here we witnessed the first chimeric recombinant <span id=\"urn:enhancement-1a0803fd-abed-431a-b237-178b871a5210\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/dna-cloning\">DNA cloning<\/span> <span id=\"urn:enhancement-4acb6c57-30ea-4a0b-8da2-5a422cc5f311\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/sv40\">SV40<\/span> molecule into plasmid DNA.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Where the 60&#8217;s laid the foundations, we saw the 70&#8217;s start to build an impressive structure.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1970 &#8211; Hamilton Smith discovers Type II restriction enzymes\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>In 1970, whilst studying the bacterium <span id=\"urn:enhancement-d62521d7-7ff4-4b27-9de6-64774e680b4d\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/haemophilus-influenzae\">Haemophilus influenzae<\/span> Rd, Smith discovered Type II <span id=\"urn:enhancement-73a68af6-989f-4194-9a7a-54bc5b18b580\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/restriction-enzymes\">restriction enzymes<\/span> (Himd II).<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>This was quickly followed by the discovery of\u00a0 6 <span id=\"urn:enhancement-685975a7-1a78-4082-ad68-d48d346648f7\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/single-stranded\">base pair<\/span> phage <span id=\"urn:enhancement-df80fd7e-c59c-47a3-8cd3-345ed71d7ff0\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/dna-sequence\">DNA sequences<\/span> in Himd.\u00a0The work that Smith and his team did later <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4081073\/\">became the groundwork for the genetic engineering therapy that became CRISPR.\u00a0 <\/a>\u00a0<a href=\"https:\/\/apkticket.com\/netflix-mod\/\">Netflix<\/a><\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1971 &#8211; Gene Splicing breakthroughs create new opportunities to create Recombinant DNA (rDNA)<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>Using a method now known as the &#8216;cut and slice&#8217; technique,<a href=\"http:\/\/www.genomenewsnetwork.org\/resources\/timeline\/1972_Berg.php\"> Paul Berg<\/a>, broke new ground and became the first person to create recombinant DNA from two different species.\u00a0\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>This discovery that DNA from different species could be &#8216;<span id=\"urn:enhancement-77eca6a1-3ad2-4f03-a415-ed4ac41802da\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/splicing\">spliced<\/span>&#8216; together paved the way for nearly all the genetic engineering we know today. Without Berg, this field would not exist.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Berg was later awarded a Nobel prize for &#8220;his fundamental studies of the biochemistry of nucleic acids, with particular regard to recombinant DNA.&#8221;<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1972: DNA mapping is made possible by Type II restriction enzymes\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>Daniel Nathans used <span id=\"urn:enhancement-735ca17e-8cee-42e5-9540-3fef6b18d2b3\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/restriction-enzymes\">restriction enzymes<\/span> on the <span id=\"urn:enhancement-1cfa2e72-0657-4631-8420-3f9d540920f2\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/sv40\">SV40<\/span> viral genome. By doing this he discovered it was possible to slice it into 11 different parts. Taking his experiments further Nathans found that <span id=\"urn:enhancement-8c1d91a2-2eb1-4fa2-84f4-dde5d69caed5\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/restriction-enzymes\">restriction enzymes<\/span> could play a huge part in DNA mapping.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>His experiments opened doors for further researchers to fully map chromosomes within DNA and all the discoveries that came with that. Nathans was awarded <a href=\"https:\/\/www.nobelprize.org\/prizes\/medicine\/1978\/summary\/\">a Nobel prize<\/a> for his contributions to the world of medicine in 1978.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1974: Genetic engineering experiments a banned by the National Academy of Sciences, pending an ethical review\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>As the field of genetic engineering began to expand and make huge leaps forward,<a href=\"http:\/\/www.els.net\/WileyCDA\/ElsArticle\/refId-a0005613.html\"> the National Academy of Sciences called for a moratorium on the field<\/a>. The public was beginning to get nervous about the experiments, worrying that scientists would use their new discoveries to create superbugs or partake in <span id=\"urn:enhancement-992a3963-0f82-49de-a911-f33eaeacb176\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/eugenics\">eugenics<\/span>.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>During this ban Paul Berg held a conference, attended by over 100 leaders in the field, to discuss the ethical parameters of genetic engineering.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>At this conference, <a href=\"https:\/\/www.nobelprize.org\/prizes\/medicine\/1958\/lederberg\/biographical\/\">Joshua Lederberg<\/a> shared his vision for a future where genetic engineering could cure many diseases that were killing thousands at the time.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Many claim this conference is what led to the change in opinions surrounding <span id=\"urn:enhancement-2ca1d5c1-5348-498c-912a-6d6552c546f2\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/molecular-genetic\">molecular genetic<\/span> experimenting.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1975: Merging of myeloma cells leads to revolutionizing modern genetic diagnostics and immunology treatments<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>After working separately on B cell experiments, scientists <a href=\"https:\/\/www.immunology.org\/kohler-and-milsteins-hybridoma-technology-1975\">George Kohler &amp; C\u00e9sar Milstein<\/a> teamed up. Their work together led to the discovery that merging <span id=\"urn:enhancement-0f1eeec1-1d8c-49b0-8c0e-18cf73703064\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/myeloma\">myeloma<\/span> cells would lead to fast reproducing, antibody growing cells.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Their discovery led to a complete change in modern diagnostic methods and treatments.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>1980s<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignright size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4886\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/3.1980s.png\" alt=\"3.1980s\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/3.1980s.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/3.1980s-300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>The 1980s is the era of using genetic engineering to solve human problems through vaccines, particularly doing this using human DNA.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1981: DNA microinjection used on an animal for the first time\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>In 1981, <a href=\"https:\/\/ohiouniversitymedicine.com\/2016\/05\/18\/40-things-to-know-the-worlds-first-transgenic-mammal-was-developed-here\/\">Ohio University<\/a> became the first lab in the world to perform DNA microinjection on an animal.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>In this experiment, they created a transgenic animal (this simply means an animal that has had its DNA &#8216;<span id=\"urn:enhancement-305dadbb-5ecd-4cee-81d4-1a284a2a76c9\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/splicing\">spliced<\/span>&#8216; with DNA from another animal).\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>This experiment was led by Thomas Wagner.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>He and his team introduced DNA from a rabbit into a mouse. This was a founding experiment in the world of transgenic animal experiments.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1982: The first synthetic drug is manufactured and put on the market\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>Up until 1982, all the <span id=\"urn:enhancement-b87cd6a1-47bb-409b-8eb9-7926567f6fef\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/insulin\">insulin<\/span> that was being used to treat those with Type 1 <span id=\"urn:enhancement-8d32d706-e9db-4c27-beb8-cc0833f712f0\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/diabetes\">diabetes<\/span> needed to be harvested from animals. The team at Genentech, led by Dennis Kleid, was able to produce synthetic <span id=\"urn:enhancement-0ceddfa5-6931-4874-a8c7-7b87f6423765\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/insulin\">insulin<\/span> identical to human <span id=\"urn:enhancement-07d2e7bd-2263-42f5-abff-e500e049b9c7\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/insulin\">insulin<\/span> and much more effective than animal <span id=\"urn:enhancement-09301f44-d662-4cf2-b321-aa63296e5ecf\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/insulin\">insulin<\/span>.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Not only was this saving human lives, but this development was believed to save the lives of 56 million animals a year. This was a breakthrough moment for health boards approving synthetic medicine.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1983: polymerase chain reaction opens new doors\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>The <span id=\"urn:enhancement-27bf22d4-5e38-49df-90f4-c003cd6d4842\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/polymerase\">polymerase<\/span> chain reaction (PCR) was discovered in 1983. This system can be used to make specific <span id=\"urn:enhancement-0cc044cc-755f-4c8c-b3bb-412b6d91f55b\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/dna-sequence\">DNA sequences<\/span> to make copies of itself. This reaction can be used to create thousands of copies. Some sequences can even make millions of copies of themselves.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>It was this discovery that made future DNA experiences possible. Being able to do multiple experiments on identical pieces of DNA made experiments with DNA less time consuming and far less expensive. Previously, cloning DNA involved cutting the DNA into thousands of pairs, and regrowing them in <span id=\"urn:enhancement-64e12f61-9bbd-4fe7-a1f6-58db2a33370d\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/bacterial\">bacteria<\/span> colonies.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1985: Recombinant vaccines are approved by the first medical body\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>Before 1986, doctors were using a <span id=\"urn:enhancement-1ffb4495-df57-48ec-b431-2df946a4fe17\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/vaccine\">vaccine<\/span> derived from blood to treat <span id=\"urn:enhancement-657643ed-b79c-4cf7-8a4c-856142a98c7d\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/hepatitis-b\">Hepatitis B<\/span>. In around 1983,\u00a0 Pablo D. T. Valenzuela began to develop a <span id=\"urn:enhancement-602c9afe-a8bc-4330-9b69-c8a6e5e394c9\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/yeast\">yeast<\/span>-based recombinant <span id=\"urn:enhancement-ee0e3f97-d026-4510-87b5-1c64c5bfd51a\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/vaccine\">vaccine<\/span> to treat the illness.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>This <span id=\"urn:enhancement-47fc6241-f550-4b8a-9b03-3addb333d0aa\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/vaccine\">vaccine<\/span> was so successful that the blood vaccines were removed from the medical practice. This <span id=\"urn:enhancement-a9ea3385-601c-4e18-8f58-41f3d523a864\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/vaccine\">vaccine<\/span> led to a wave of newly formulated recombinant vaccines, some of which are still in use today.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1998: GMO crop trials begin\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>A <span id=\"urn:enhancement-f21261c9-8f1e-4ef8-aaa7-1ea9ead24fe7\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/genetically_modified_organism\">GMO<\/span> strain of corn was approved for sale in 1998. This corn had been modified to produce higher yields, put off pests, and be unaffected by the use of pesticides (this has come to have a negative effect on the ecosystems on American farms).\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>1990s<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignleft size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4887\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/4.1990s.png\" alt=\"4.1990s\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/4.1990s.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/4.1990s-300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>In this decade we see the birth of the Human <span id=\"urn:enhancement-737d924f-eb44-4328-8544-d671b2a9e8dd\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/genome-project\">Genome Project<\/span>.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>This project, over 13 years mapped out over 20,000 genes.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>The work done in this decade laid the groundwork for the world-changing research we are witnessing today.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1990: Human Genome Project is launched\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>This thirteen-year-long project is the foundation for nearly all modern day <span id=\"urn:enhancement-fab49ac8-d5f1-428d-b62c-01da3e8d4fab\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/molecular-biology\">molecular biology<\/span>.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>During this project over 20,000 genes were discovered and mapped out.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1993: CRISPR cells are discovered\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>In 1993 Francisco Mojica discovered <span id=\"urn:enhancement-3379996a-8d72-43ed-87f3-6e19c777e05c\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> (clusters of regularly interspaced short palindromic repeats) cells. Over the next 25 years, his work in this subject created a wealth of knowledge that was drawn upon to create the <span id=\"urn:enhancement-48b586e9-8363-4bb0-a3a4-1313d3ab7413\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-CAS9 genome editing tools.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1994: GMO tomato company tanks\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>This was the year that we saw the second major launch of <span id=\"urn:enhancement-c3eb4b00-7ddf-4eec-9484-70980e29a12e\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/genetically_modified_organism\">GMO<\/span> crops. These tomatoes were engineered to never over ripen. However, the company eventually tanked after consumers began to grow concerned about the effects <span id=\"urn:enhancement-3b8e821f-e9d3-4a9c-ade2-6851cd79ab36\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/genetically_modified_organism\">GMO<\/span> could have on their food and them.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1996: Dolly the Sheep\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>In one of the most impressive and shocking feats of genetic engineering, scientists used a <span id=\"urn:enhancement-c3a44b8e-94ba-4b1c-b7b4-d5f972580cf6\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/stem-cell\">stem cell<\/span> to clone an entire sheep. The sheep was named Dolly. In the following decade, scientists began to clone endangered animals.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>1999: The first human Chromosome is sequenced\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>As part of the Human <span id=\"urn:enhancement-cf64315b-a6e5-4676-8dd3-bbdb6666c412\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/genome-project\">Genome Project<\/span>, scientists completely map out a chromosome for the first time. This chromosome was Chromosome 22. The methods that were used to achieve this are still employed today (although they are now done electronically).\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>2000s<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignright size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4888\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/5.2000s.png\" alt=\"5.2000s\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/5.2000s.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/5.2000s-300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>In this decade we began to see the true benefits of the work done by the Human <span id=\"urn:enhancement-a1fe4dfd-b39d-4e5a-9233-76064fa75276\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/genome-project\">Genome Project<\/span>.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p><span id=\"urn:enhancement-0d3408d1-c779-422c-991d-15201c84660e\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/genetic-engineering\">Genetic engineering<\/span> turned its eyes towards understanding and curing genetic diseases.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>We see the first cloning of an endangered animal, and the approval of many life changing genetic treatments.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>2001: Gene-targeted drug therapy is approved for the first time\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>This year the FDA approved the first gene-targeting drug. This drug was a treatment for adults with Leukemia and is still used today.\u00a0\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>2003: The first GMO pet goes on sale\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>UK pet store Pets At Home begins selling &#8216;Glow-fish&#8217;. These <span id=\"urn:enhancement-88582093-9123-40c4-bc07-267380820f58\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/genetically_modified_organism\">GMO<\/span> fish were able to glow in the dark, thanks to the addition of bioluminescence. This type of technology has since been harnessed to help farmers in developing countries.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>2006: First preventative cancer vaccine approved\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>In its clinical trials, the <span id=\"urn:enhancement-5f92deef-a02c-44ab-8f8d-dd31a255aa64\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/hpv-vaccine\">HPV vaccine<\/span> was proved to be 99% effective at preventing pre-cancers from developing. These pre-cancers cause 70% of <span id=\"urn:enhancement-4df5c3b9-84d4-4b62-8354-56176ea28d99\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/cervical-cancer\">cervical cancer<\/span> cases.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>After its approval, the <span id=\"urn:enhancement-247d78c9-3bb1-4aa6-b902-9d5c918d56b3\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/vaccine\">vaccine<\/span> was rolled out to teenage women across the world. It was even administered on mass in UK schools.\u00a0 It is still currently the only approved preventative cancer <span id=\"urn:enhancement-4e12bc68-f39e-4b3c-8e89-ede089fba54d\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/vaccine\">vaccine<\/span>.\u00a0\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>2006: Stem cell research jumps forward\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>In the same year, the first iPSCs ( induced pluripotent stem cells) were created. Dr. Shinya Yamanaka and his team isolated fibroblasts and they were able to reprogram them into a <span id=\"urn:enhancement-d668404e-3815-4966-b1c8-cecd64b96cfe\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/stem-cell\">stem cell<\/span> state. This discovery opened up a new world of <span id=\"urn:enhancement-17cf9f39-a6d1-43db-8d01-8cc245043a70\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/stem-cell\">stem cell<\/span> research.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>2010s<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignleft size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4889\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/6.2010s.png\" alt=\"6.2010s\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/6.2010s.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/6.2010s-300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>In the 2010s genetic breakthroughs happen faster than the pioneers of the 1960s could have ever imagined.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>In this decade we see the invention of <span id=\"urn:enhancement-b862f132-6bb4-48a7-96db-69f7e9805ee9\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> and the first approval of genome editing on humans\u2026 with very promising results.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>2010: Synthetic bacteria becomes world&#8217;s first living synthetic creation\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>After years of experimentation, <span id=\"urn:enhancement-3cf98313-ebd4-4c1f-8eea-0a00f08a9cb2\" class=\"textannotation disambiguated wl-person\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/craig-venter\">Craig Venter<\/span> and his team were able to create an M. capricolum cell synthetically.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>This <span id=\"urn:enhancement-bcfbe5ca-b604-41a2-89a4-52fa3523d014\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/bacterial\">bacteria<\/span> was the first synthetic life form (meaning something that was not born or evolved).\u00a0\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>2011: TALEN is discovered\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>Further experiments allowed scientists to build on the revolutionary work of ZNFs, and discovered TALENs. TALENs are more accurate than ZNFs, and are less cytotoxic to the host cells.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>2012: The CRISPR tool is born\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p><span id=\"urn:enhancement-1ec50e0a-5426-4fc3-9d55-8fd61959c18b\" class=\"textannotation disambiguated wl-person\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/jennifer-doudna\">Jennifer Doudna<\/span> and Emmanuelle Charpentier develop <span id=\"urn:enhancement-e62fce7a-1b92-4eb0-b52a-aefb58c94037\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>, a genome editing tool that heralds a new age of <span id=\"urn:enhancement-17ec8da9-58eb-4fe8-8785-767d4d46ec34\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/molecular-biology\">molecular biology<\/span> and medicine. Early experiment results suggest <span id=\"urn:enhancement-20ddf8b1-1e71-4088-b4ac-bd19e11d2a56\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> treatment could replace chemotherapy when approved for human use.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>2014: Gene drives?\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>As <span id=\"urn:enhancement-7c6c4ed1-6374-4c8c-98ef-057a689e7fa8\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> continues to revolutionize the world of <span id=\"urn:enhancement-a7917a06-d819-4f3d-8a38-20aec31d77b1\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/molecular-biology\">molecular biology<\/span>, the field begins to talk about what could be done with a tool like this. Kevin Esvelt suggests that <span id=\"urn:enhancement-3cfd229f-156f-428a-806b-1b9b14a3cbe7\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> could be used to great gene drives &#8211; these could &#8216;bypass evolution&#8217;.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Esvelt suggests that they could be used to eradicate malaria from the mosquito population. But that there are many ways that gene drives could be used dangerously. And that extensive testing should take place before releasing any experience into a real ecosystem.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>2015: GMO salmon sold in Canada\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p><span id=\"urn:enhancement-9e27099b-06ad-4fc7-bc56-198f7179a117\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/genetically_modified_organism\">GMO<\/span> had been happening to crops for years, but 2015 marked the first <span id=\"urn:enhancement-a6e202fc-4300-4054-a730-9a55ea18de28\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/genetically_modified_organism\">GMO<\/span> animal product to hit the market. In Canada, a company began selling <span id=\"urn:enhancement-7cdd21ea-431a-4123-ae3e-f5f663d5f60e\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/genetically_modified_organism\">GMO<\/span> salmon and claimed that products like this could solve the world&#8217;s overfishing problems.\u00a0\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>2015: Controversial CRISPR trial takes place on human embryo\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>Scientists in China attempted to remove a hereditary blood disease from a human embryo. This was three years before any government approved human <span id=\"urn:enhancement-8a7bdf4d-bce9-47e8-927f-fc5445922cf8\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> trials. The experiment was not covered by Western journals who labeled it unethical.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>2017: CAR-T therapy is approved for human trials<\/strong>\u00a0<\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>Two different CAR-T experiments were approved in 2017. One to treat children with Leukemia and the other to treat adults with Lymphoma. Trials on animals showed that CAR-T could be a non-toxic replacement for Chemotherapy. Multiple studies showed CAR-T&#8217;s ability to completely destroy tumors in less than two weeks.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>2018: Human CRISPR trials are approved\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>Two leading <span id=\"urn:enhancement-bbe602c0-7642-482a-98b5-f879e298eb46\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> development companies are given permission to trial human <span id=\"urn:enhancement-e005cf01-b27e-48e8-b949-2bfd7b109bcd\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> treatments together. These experiments were designed to treat Sickle Cell disease. (Good news about this trial is coming later in the article)<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>2019: Prime editing experiments prove successful\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>This new extension of the <span id=\"urn:enhancement-b51cf912-e6b4-4e38-b186-10cc2796b770\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> technique halved the size of the smallest incision thought possible. This technique makes it possible to make <span id=\"urn:enhancement-b1c74843-1d55-4cc8-aa08-dee35e2b2d68\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/single-stranded\">single stranded<\/span> cuts when previously only <span id=\"urn:enhancement-7325e80f-c49d-414a-a4c4-d5cf869fd844\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/single-stranded\">double stranded<\/span> cuts were considered possible. Reducing the damage to the DNA when cut to next to nothing.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>Present\u00a0<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignright size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4890\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/7.Present-.png\" alt=\"7.Present\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/7.Present-.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/7.Present--300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>2020 was a huge year for <span id=\"urn:enhancement-bc2e1bb8-9d37-4d21-b977-2bffb3645c11\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Emmanuelle Charpentier and <span id=\"urn:enhancement-e92d35b6-2bf7-4ca9-9e90-79a6b03ccde2\" class=\"textannotation disambiguated wl-person\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/jennifer-doudna\">Jennifer Doudna<\/span> won the Nobel Prize for their time spent developing <span id=\"urn:enhancement-7bb3acbf-018a-4657-9b69-66f22efb7e30\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> and their contributions to Chemistry.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>In the middle of 2020, a series of promising <span id=\"urn:enhancement-c80179e9-7ef1-4d99-b81c-9cbfe33877da\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> trial results were released.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>The first person to undergo Sickle Cell <span id=\"urn:enhancement-fe496392-0fc3-4e73-a033-9bce48bf8567\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> treatment, Victoria Gray, was showing very positive results post-treatment.\u00a0\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Later in the year, results showed that all 10 people who had undergone this treatment were showing huge signs of improvement.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>All showed improvements in fetal <span id=\"urn:enhancement-2ae5a9c0-6ce0-4644-96a0-ddde0c3c8aad\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/hemoglobin\">hemoglobin<\/span> levels, no longer required blood transfusions, and had long pain-free periods.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p id=\"3\">CRISPR<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1000\" height=\"401\" class=\"wp-image-4878\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/3.CRISPR.png\" alt=\"3.CRISPR\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/3.CRISPR.png 1000w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/3.CRISPR-300x120.png 300w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/3.CRISPR-768x308.png 768w\" sizes=\"(max-width: 1000px) 100vw, 1000px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>Now that we have covered the history of genetic editing, it&#8217;s time to take a deeper look into <span id=\"urn:enhancement-4ba06b7f-85eb-499c-8393-bae6ece74a38\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>. In this section we will be covering all the basics you need to know about <span id=\"urn:enhancement-d4e9158c-0359-48a7-a1e7-eeb0843c9ed3\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> to understand the more complex things we will cover later in the article.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>What does it stand for?<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignleft size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4891\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/8.What-does-it-stand-for.png\" alt=\"8.What does it stand for\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/8.What-does-it-stand-for.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/8.What-does-it-stand-for-300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p><span id=\"urn:enhancement-2788d48e-089f-4f04-a4cd-e00c1ea0ed9a\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> (which is pronounced &#8220;crisper&#8221;) is both a DNA cycle and a shorthand for the <span id=\"urn:enhancement-ba3f70fb-095e-4fcc-882d-1cd7c62ea72b\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-CAS9 genome editing technology, and stands for the following:\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>C &#8211; Clustered<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>R &#8211; Regularly\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>I &#8211; Interspaced\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>S &#8211; Short\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>P &#8211; Palindromic\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>R\u00a0 &#8211; Repeats<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p><span id=\"urn:enhancement-85a7e348-c609-4567-ad43-9ebe8d693cc2\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> is the name of the <span id=\"urn:enhancement-092e259e-fb0d-4ad8-8c87-2a5240f1c727\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/bacterial\">bacterial<\/span> defense system that makes up the basis of the <span id=\"urn:enhancement-8a4d1d09-e7f7-4859-998b-ca7bcc2291b4\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-CAS9 genome editing system technology.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>What is CRISPR?<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignright size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4892\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/9.What-is-CRISPR.png\" alt=\"9.What is CRISPR\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/9.What-is-CRISPR.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/9.What-is-CRISPR-300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>As mentioned above <span id=\"urn:enhancement-eb82a380-5e23-4768-b3b8-00c205480506\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> is the moniker of the <span id=\"urn:enhancement-9530c68d-28a6-412a-9104-11e33e26d51c\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/bacterial\">bacterial<\/span> defense system which the <span id=\"urn:enhancement-bdf17dc9-4cf2-4c6a-b64a-84893f1e8a2a\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-CAS9 genome editing technology is based on.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p><span id=\"urn:enhancement-100f3482-28ad-49dd-8144-075c862c2349\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> is often used in the industry as a general name to cover any type of technology that targets and edits long stretches of DNA at specific points.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>This can lead to a little confusion when trying to view the field of genome editing from the outside.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>So, how were CRISPR cells discovered?\u00a0<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<p>Francisco Mojica first discovered <a href=\"https:\/\/www.broadinstitute.org\/what-broad\/areas-focus\/project-spotlight\/crispr-timeline\">CRISPR in archaea<\/a>. He believed that CRISPRs were a key part of <span id=\"urn:enhancement-434621a5-bf52-458e-b68d-758ed9975361\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/bacterial\">bacterial<\/span> immune systems.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>This was later proved to be correct when CRISPRs were discovered in <span id=\"urn:enhancement-d29c6d38-be05-4cf0-8fa5-96593d4b893a\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/bacterial\">bacteria<\/span>.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>They are made up of a sequence of repeating genetic codes, in between the reparations are remnants of genetic code from previous threats the <span id=\"urn:enhancement-ece00ae0-3e5c-4739-8c37-64620a551b42\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/bacterial\">bacteria<\/span> has encountered. Mojica believed that these old DNA parts were kept to allow the immune system to identify and destroy attackers quickly.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>These were later developed into a genome editing and diagnostic system. Which we will cover more next.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p id=\"4\">What is CAS9?<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>In this section, we will cover two things: what CAS9 is and what <span id=\"urn:enhancement-76a1bed0-16c9-4dad-9c2d-ea7a8e3c4fb0\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-CAS9 is.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignleft size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4893\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/10.What-is-CAS9.png\" alt=\"10.What is CAS9\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/10.What-is-CAS9.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/10.What-is-CAS9-300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>What is CAS9?\u00a0<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>CAS9 is the protein at the heart of the <span id=\"urn:enhancement-5904c514-74dc-43d6-8426-77b3b7e26887\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> system.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>CAS9 is able to take on a gRNA (guide <span id=\"urn:enhancement-953d771c-386b-4eb5-a3cf-ae9b0897c9c0\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/rna\">RNA<\/span>) and remove any genes that match this gRNA from the subject&#8217;s DNA.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Originally, this protein was a part of <span id=\"urn:enhancement-1b2438aa-99d2-4a97-8776-e7dae6a25da1\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/bacterial\">bacterial<\/span> immune systems.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Now it has harnessed to be an efficient and accurate tool in the world of genome editing.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>What is CRISPR-CAS9?<\/strong><\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p><span id=\"urn:enhancement-12823c2d-79ff-4f78-aac5-29bee9f5b14b\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-CAS9 is a trailblazing genome editing tool developed by <span id=\"urn:enhancement-49fa35b6-7e92-42fb-90eb-ef545ca61a74\" class=\"textannotation disambiguated wl-person\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/jennifer-doudna\">Jennifer Doudna<\/span>, Emmanuelle Charpentier, and their team.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>This tool harnesses the ability of the CAS9 protein. With CAS9 they are able to edit genes in a non-toxic, non-<span id=\"urn:enhancement-1d4f6a3c-d3e3-400c-9ee1-b511596768e3\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/invasive\">invasive<\/span> way. Their system has revolutionized the way we approach genome editing in the 21st century.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>\u00a0<span id=\"urn:enhancement-9fb4f14b-64f1-4cf0-be5f-094ee1b4e872\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>&#8216;s main selling point is its ability to precisely target and cuts genetic material. How precisely? Well, the difference is similar to trying to pick up a grain of rice with tweezers and 2 boxing gloves.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>When was CRISPR-CAS9 developed?\u00a0<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignright size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4895\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/12.When-was-CRISPR-developed.png\" alt=\"12.When was CRISPR developed\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/12.When-was-CRISPR-developed.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/12.When-was-CRISPR-developed-300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p><span id=\"urn:enhancement-3fab46b7-29dc-41a9-8a54-d2b6efc4aac4\" class=\"textannotation disambiguated wl-person\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/jennifer-doudna\">Jennifer Doudna<\/span>, Emmanuelle Charpentier, and their team developed their <span id=\"urn:enhancement-3bd4a75e-6f57-468f-b375-d23a1bc7c5a0\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-CAS9 in the early 21st century.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Their work builds on the discovery of <span id=\"urn:enhancement-b2af8c39-1730-4dc2-b1e0-21ca370b8fd7\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> cells in e-colli and yogurt.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>In 2012 Doudna and Charpentier launched their <span id=\"urn:enhancement-6c56f839-ca78-4789-a029-2b5a9609a046\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-CAS9 system.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>To this day there are still a huge amount of rules and regulations surrounding the use of <span id=\"urn:enhancement-d2639b3a-1a0c-43d5-a8b8-f422cee9c6f5\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>This is mostly the result of scientific research that was done in Russia and China in the early days of <span id=\"urn:enhancement-e153345c-e4ba-4f2f-b98d-c59be31d5e94\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-CAS9 that was deemed unethical by the scientific community.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Despite these restrictions, most industries are still backing <span id=\"urn:enhancement-2b761240-a46c-4ec8-abfa-429973a9498c\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> as the future of microbiology.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>And in the last 5 years, we have seen a series of human trials being approved. 2020 gives us a series of really promising results for the first set of trials.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h2 class=\"wp-block-heading\"><strong>What is the goal of CRISPR-CAS9 technology?<\/strong><\/h2>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1000\" height=\"400\" class=\"wp-image-4879\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/4.What-is-the-goal-of-CRISPR-CAS9-technology.png\" alt=\"4.What is the goal of CRISPR-CAS9 technology\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/4.What-is-the-goal-of-CRISPR-CAS9-technology.png 1000w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/4.What-is-the-goal-of-CRISPR-CAS9-technology-300x120.png 300w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/4.What-is-the-goal-of-CRISPR-CAS9-technology-768x307.png 768w\" sizes=\"(max-width: 1000px) 100vw, 1000px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p><span id=\"urn:enhancement-c7ee2d9c-eb9e-4b75-afef-96443bea8868\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> truly has a limitless potential and for that reason, every major industry is investing heavily in it. From biofuel companies to people who sell legal highs. We could see <span id=\"urn:enhancement-2663b11e-b6f8-44f5-87ef-6ca13f348e0f\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-CAS9 having an impact on our lives at every level.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p><span id=\"urn:enhancement-f97ccb13-9d1e-4830-bdd6-679e78514a3d\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> could be used for any of the following:\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<ul class=\"wp-block-list\">\r\n<li>Treating terminal diseases\u00a0<\/li>\r\n<li>Producing sustainable biofuels\u00a0<\/li>\r\n<li>For eradicating animal and insect-borne diseases\u00a0<\/li>\r\n<li>Creating <span id=\"urn:enhancement-e82898ef-bae2-4089-bd77-728afb941c9e\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/bacterial\">bacteria<\/span> that could eat and digest non-biodegradable materials like plastics\u00a0<\/li>\r\n<li>Cloning endangered and extinct species\u00a0<\/li>\r\n<li>Creating crops that can be grown in arid conditions\u00a0<\/li>\r\n<\/ul>\r\n<p>\r\n\r\n<\/p>\r\n<p>With the invention of the <span id=\"urn:enhancement-8e970be7-388e-47ad-83d1-e8082b10508e\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-CAS9 tool, <span id=\"urn:enhancement-3db3c441-e51d-4dff-8785-d631a7d4048e\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/gene_therapy\">gene editing<\/span> has become easier, cheaper, more accurate and less dangerou,s. The possibilities are truly endless.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p id=\"5\">How does CRISPR gene editing work?<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1000\" height=\"401\" class=\"wp-image-4880\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/5.How-does-CRISPR-gene-editing-work.png\" alt=\"5.How does CRISPR gene editing work\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/5.How-does-CRISPR-gene-editing-work.png 1000w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/5.How-does-CRISPR-gene-editing-work-300x120.png 300w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/5.How-does-CRISPR-gene-editing-work-768x308.png 768w\" sizes=\"(max-width: 1000px) 100vw, 1000px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>Despite taking decades to develop, <span id=\"urn:enhancement-273d9047-b350-4695-b7ad-20fb970a499a\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span> <span id=\"urn:enhancement-67127feb-75c1-46a2-b87b-7f38fc082f2b\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/gene_therapy\">gene editing<\/span> is a surprisingly simple process. In the following section we were going to talk you through the process of the <span id=\"urn:enhancement-953a5a70-5f5d-4c10-a7bf-10f92bb71e02\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-CAS9 <span id=\"urn:enhancement-101de559-8e32-4f83-b304-83218ed04e8a\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/gene_therapy\">gene editing<\/span> tool.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>As mentioned before, these systems are currently going through trials for treating sickle cell disease and leukemia in humans. We will be focusing our example around that process.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Here are some important terms to be aware of:\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p><strong>gRNA &#8211; <\/strong>guiding <span id=\"urn:enhancement-9d1e8b51-4068-448c-b5c5-f9a5d55da274\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/rna\">RNA<\/span>, this will be a copy of the gene that scientists want to remove\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p><strong>crRNA &#8211;<\/strong> a gene to replace the genes that are being removed<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p><strong>CAS9 &#8211; <\/strong>the star of the show. This is a protein that is able to target and remove the gRNA without damaging anything else in the DNA. It is incredibly accurate. It removes the gRNA copies with small pincers that can perform single stand cuts on pieces of DNA.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>Selecting an organism to manipulate\u00a0<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<p><span id=\"urn:enhancement-61e6fee4-0ca1-49a6-a7bb-a3ca8af103ae\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/crispr\">CRISPR<\/span>-CAS9 can be used on nearly any type of cell, so their choices for this section are near limitless.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>If an experiment was being done to try and cure sickle cell disease, we would begin by finding someone with sickle cell disease.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>Selecting a gene or DNA sequence we wish to manipulate<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<p>Next,we would identify the gene that causes a person to have sickle cell disease.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>The last most likely already has a copy of this on file.\u00a0\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>Selecting the CRISPR and gRNA\u00a0<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignleft size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4898\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/15.Selecting-the-CRISPR-and-gRNA-.png\" alt=\"15.Selecting the CRISPR and gRNA\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/15.Selecting-the-CRISPR-and-gRNA-.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/15.Selecting-the-CRISPR-and-gRNA--300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>Now, we will select the CAS9 protein.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>As we know this protein is incredibly accurate and is able to carry crRNA and gRNA while it moves through the body.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>The lab will then decide upon the exact gene they want the CAS9 to replace, this will be our gRNA.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>A guiding <span id=\"urn:enhancement-1eb268f4-5879-46c6-a897-8fd0b0b21ee1\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/rna\">RNA<\/span> that will dictate to the CAS9 protein which gene it should be removed from the body.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>Constructing the gRNA or sgRNA by synthesis and cloning<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignright size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4899\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/16.Constructing-the-gRNA-or-sgRNA-by-synthesis-and-cloning.png\" alt=\"16.Constructing the gRNA or sgRNA by synthesis and cloning\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/16.Constructing-the-gRNA-or-sgRNA-by-synthesis-and-cloning.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/16.Constructing-the-gRNA-or-sgRNA-by-synthesis-and-cloning-300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>The lab will then go through the process of cloning out gRNA. Once this is done the gRNA will be combined with the CAS9 protein.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Sometimes a sgRNA\/crRNA will be selected. This will be a second gene carried by the CAS9 protein.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>This gene will be inserted into the place of the gene that held the sickle cell issues.\u00a0Why do we need to use a crRNA? Isn&#8217;t removing the bad gene enough?\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Actually, the safest way to carry out this process is to replace what you remove. If you do not do this then you have no control over how the DNA repairs itself.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>It often does a bad job, as the human body isn&#8217;t used to having to do this kind of repair work. Not replacing a missing gene can cause <span id=\"urn:enhancement-e3cfeada-c903-4b51-8567-8777ef80fbed\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/mutations\">mutations<\/span> and other complications further down the line.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>The cRNA is also combined with this CAS9 protein.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>Delivering the sgRNA and CAS9 to the target cell\u00a0<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignleft size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4901\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/18.Delivering-the-sgRNA-and-CAS9-to-the-target-cell-.png\" alt=\"18.Delivering the sgRNA and CAS9 to the target cell\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/18.Delivering-the-sgRNA-and-CAS9-to-the-target-cell-.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/18.Delivering-the-sgRNA-and-CAS9-to-the-target-cell--300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>When the CAS9 cocktail is prepared it is then injected into the patient.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>The CAP9 will then use the gRNA to find the sickle cell gene.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>It will use its two pincer-like arms to slice this gene from the body, and replace it with the crRNA.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>What you should be left with is a sickle cell free, recombinant DNA sequence.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Replacing the bad gene does not only prevent <span id=\"urn:enhancement-b83624d3-bb8d-41b8-af2a-bf12334eea4d\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/mutations\">mutations<\/span>, but it allows the DNA to repair itself fast.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>Validating the manipulation\u00a0<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignright size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4902\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/19.Validating-the-manipulation-.png\" alt=\"19.Validating the manipulation\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/19.Validating-the-manipulation-.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/19.Validating-the-manipulation--300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>Finally the lab will take new samples from the patient every few months.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>They will compare them to their original samples to measure what effect the lack of sickle cell gene is having on the boy.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h4 class=\"wp-block-heading\"><strong>Real work sickle cell experiments<\/strong>\u00a0<\/h4>\r\n<p>\r\n\r\n<\/p>\r\n<p>There are currently real human sickle cell trials <a href=\"https:\/\/www.nature.com\/articles\/d41586-020-03476-x\">going on.<\/a><\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>These trials have had very promising results.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Including all 10 patients no longer needing blood transfusions and all witnessing rises in their fetal <span id=\"urn:enhancement-a4324bc7-6c84-440f-bc97-bda2fa7f60f7\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/hemoglobin\">hemoglobin<\/span> levels.\u00a0\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p id=\"6\">What is CRISPR used for?<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1001\" height=\"401\" class=\"wp-image-4881\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/7.What-is-CRISPR-used-for.png\" alt=\"7.What is CRISPR used for\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/7.What-is-CRISPR-used-for.png 1001w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/7.What-is-CRISPR-used-for-300x120.png 300w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/7.What-is-CRISPR-used-for-768x308.png 768w\" sizes=\"(max-width: 1001px) 100vw, 1001px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>Let&#8217;s look at the current applications of the CRISPR tool.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>Using CRISPR for genome editing<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignleft size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4903\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/20.Using-CRISPR-for-genome-editing..png\" alt=\"20.Using CRISPR for genome editing.\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/20.Using-CRISPR-for-genome-editing..png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/20.Using-CRISPR-for-genome-editing.-300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>As we have covered extensively in this piece, CRISPR tools have revolutionized the world of genome editing.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>The process had previously been expensive, time consuming, and dangerous for the subject.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>By using CRISPR proteins as treatments <span id=\"urn:enhancement-de7bd561-b268-4e04-8c69-22e3f5230ae3\" class=\"textannotation disambiguated wl-creative-work\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/gmo\">GMO<\/span> processes and medical treatments can become non-<span id=\"urn:enhancement-5d85df00-bd4e-46db-888d-f886d57a79dc\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/invasive\">invasive<\/span> and a lot cheaper.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>Using CRISPR libraries for screening<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignright size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4904\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/21.Using-CRISPR-libraries-for-screening..png\" alt=\"21.Using CRISPR libraries for screening.\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/21.Using-CRISPR-libraries-for-screening..png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/21.Using-CRISPR-libraries-for-screening.-300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>CRISPR libraries are a collection of gRNAs and plasmids.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Generally, the libraries that can be bought are themed around a certain type of gene (although there are a few VERY large libraries that hold over 175,000 gRNAs in one test tube).\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Introducing one of these libraries to a controlled environment will allow you to do the following three things:\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<ol class=\"wp-block-list\">\r\n<li>Confirm theories using hypothesis-directed testing<\/li>\r\n<li>Explore gene lineage (via screening with libraries)\u00a0<\/li>\r\n<li>Parsing the effects of expressing multiple genes<\/li>\r\n<\/ol>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p id=\"7\">Benefits of CRISPR<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1001\" height=\"401\" class=\"wp-image-4882\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/8.Benefits-of-CRISPR.png\" alt=\"8.Benefits of CRISPR\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/8.Benefits-of-CRISPR.png 1001w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/8.Benefits-of-CRISPR-300x120.png 300w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/8.Benefits-of-CRISPR-768x308.png 768w\" sizes=\"(max-width: 1001px) 100vw, 1001px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>We have mentioned previously that the possibilities of CRISPR application are nearly limitless. Let&#8217;s take a look at the things that CRISPR has already started to be used for:\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>Remove malaria from mosquitoes<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignleft size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4905\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/27.Remove-malaria-from-mosquitos..png\" alt=\"27.Remove malaria from mosquitos.\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/27.Remove-malaria-from-mosquitos..png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/27.Remove-malaria-from-mosquitos.-300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>One of the first CRISPR projects that captured the attention of the public with the experiments being done to try and eradicate malaria in the mosquito population.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Malaria is responsible for up to t<a href=\"https:\/\/www.medscape.com\/answers\/221134-40790\/what-is-the-mortality-rate-of-malaria\">hree million<\/a> deaths a year. Most of these deaths occur in children under 5.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Using CRISPR-CAS9 it could be possible to remove malaria-carrying gene from a group of mosquitoes.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>With the hope that malaria-free mosquitoes would become the dominant form of the species.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>If this experiment was successful it could change the lives of billions of people.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>Treating Alzheimer\u2019s disease<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignright size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4906\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/28.Treating-Alzheimers-disease.-.png\" alt=\"28.Treating Alzheimer\u2019s disease.\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/28.Treating-Alzheimers-disease.-.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/28.Treating-Alzheimers-disease.--300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>The more research we do into degenerative diseases like <span id=\"urn:enhancement-cd05e859-330f-4c82-a862-cdf11389f8b4\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/alzheimers\">Alzheimer&#8217;s<\/span> the more we have come to understand not only what causes them, but also ways we can prevent them.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>The cause of <span id=\"urn:enhancement-6e653cc6-58c2-4db7-a9f6-1cf6ac0df1d3\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/alzheimers\">Alzheimer&#8217;s<\/span> hasn&#8217;t been fully discovered yet.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>However, <span id=\"urn:enhancement-1bb2f453-c73d-4205-a4b0-77089ea99637\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/gene-editing\">gene editing<\/span> simulations with CRISPR are allowing scientists to study how removing certain genes from brain cells affects the disease&#8217;s development.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Discovering the gene that causes the disease would quickly lead to an effective CRISPR-based treatment being developed.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>Treating HIV<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignleft size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4907\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/29.Treating-HIV.png\" alt=\"29.Treating HIV\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/29.Treating-HIV.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/29.Treating-HIV-300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>In studies on monkeys, scientists have proved that CRISPR -CAS9 is capable of removing <span id=\"urn:enhancement-2ace7032-3e6d-43c8-8e29-439c3ae0b00f\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/hiv\">HIV<\/span> from cells.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>More recent studies have shown that in extracted human cells CAS9 can effectively destroy <span id=\"urn:enhancement-30713d09-ae60-4ca5-949a-8fedca960616\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/hiv\">HIV<\/span> in humans.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>The UN believes there are currently 3.5 million people living with <span id=\"urn:enhancement-2754b230-e2c5-4b33-8b81-28fbac619f6a\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/hiv\">HIV<\/span> in the world right now.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Currently, there is no cure for it, the only medication that can prevent the disease from mutating into more serious AIDs.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Being able to remove <span id=\"urn:enhancement-2adad4e9-bd36-4fdb-b17d-53d3846ca07f\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/hiv\">HIV<\/span> through a CRISPR-CAS9 would not only change lives but remove a lot of the fear and stigmatization surrounding the disease.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>Develop new drugs<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<p>Many large pharmaceutical companies are investing large amounts of money into CRISPR.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>With the hope that the technology could be used to replace expensive, painful, and <span id=\"urn:enhancement-dad62841-9fc8-4a96-8ef5-832fefcffbb5\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/invasive\">invasive<\/span> medical treatments.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>Livestock<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignright size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4909\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/31.Livestock.png\" alt=\"31.Livestock\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/31.Livestock.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/31.Livestock-300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>Chinese scientists have been working on ways to increase wool growth rates in sheep by editing their genes.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>They have also been looking at ways to increase the fat carried by cattle.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>If sheep were able to grow wool faster it is possible that less space would have to be given over to farming.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>Agricultural crops\u00a0<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<p>We have seen GMOs in crops for many decades. CRISPR offers the opportunity to irradcate disease within our crops.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Leading perhaps to more stable harvests around the world and less of a need to use pesticides.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>Develop new cancer treatments\u00a0<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignleft size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4911\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/33.Develop-new-cancer-treatments.png\" alt=\"33.Develop new cancer treatments\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/33.Develop-new-cancer-treatments.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/33.Develop-new-cancer-treatments-300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>We mentioned above that human trials are currently taking place to see if it is possible to use CRISPR to treat cancer.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Early CRISPR trials in rats have suggested that CRISPR could be used to replace chemotherapy.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>What would be the benefit of that?<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Well, CRISPR can be delivered in a single injection, it&#8217;s not toxic and much more accurate than chemotherapy.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>CRISPR can target the exact genes causing cancer.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>Unlike chemotherapy, CRISPR doesn&#8217;t cause nausea or hair loss, or any of the other traumatic side effects of chemo.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<h3 class=\"wp-block-heading\"><strong>Reduce our need for plastic<\/strong><\/h3>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"alignright size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"351\" height=\"301\" class=\"wp-image-4912\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/34.Reduce-our-need-for-plastic.png\" alt=\"34.Reduce our need for plastic\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/34.Reduce-our-need-for-plastic.png 351w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/34.Reduce-our-need-for-plastic-300x257.png 300w\" sizes=\"(max-width: 351px) 100vw, 351px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>CRISPR has presented the opportunity for scientists to edit the genes of natural materials to make them act more like plastic.<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>By changing their protein structure we can make things like <span id=\"urn:enhancement-87d5b6f7-6bf0-4e3e-8105-d09e34cc9246\" class=\"textannotation disambiguated wl-thing\" itemid=\"https:\/\/data.wordlift.io\/wl1503301\/entity\/yeast\">yeast<\/span> as strong as oil-based plastics but allow them to retain their biodegradable ability.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>It is even possible that we could genetically engineer bacteria to eat and digest plastic in the future.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p id=\"8\">Conclusion\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<div class=\"wp-block-image\">\r\n<figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1001\" height=\"401\" class=\"wp-image-4883\" src=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/9.Conclusion-Discuss-how-revolutionary-it-is-for-genome-editing-.png\" alt=\"9.Conclusion - Discuss how revolutionary it is for genome editing\" srcset=\"https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/9.Conclusion-Discuss-how-revolutionary-it-is-for-genome-editing-.png 1001w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/9.Conclusion-Discuss-how-revolutionary-it-is-for-genome-editing--300x120.png 300w, https:\/\/www.mybiosource.com\/learn\/wp-content\/uploads\/2021\/03\/9.Conclusion-Discuss-how-revolutionary-it-is-for-genome-editing--768x308.png 768w\" sizes=\"(max-width: 1001px) 100vw, 1001px\" \/><\/figure>\r\n<\/div>\r\n<p>\r\n\r\n<\/p>\r\n<p>Nothing ever has, and possibly ever will revolutionize genome editing the way CRISPR has. One of the major contributing factors to the power of CRISPR is its accessibility. Not only is it one of the cheapest forms of genetic engineering available right now, but there are huge databases that can post to you everything you need to perform your studies.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>With a tool like CRISPR genome experiments and electronic genome, screening has become possible for most labs. Even those on a very small budget. This is something that will benefit us all in the future.\u00a0<\/p>\r\n<p>\r\n\r\n<\/p>\r\n<p>CRISPR has the potential to change every area of our lives. From the food we eat, how we treat our illnesses, to forcing cross-species diseases like malaria into extinction. It&#8217;s hard to imagine all the things that CRISPR will be used for in the future. But we do know that CRISPR is already making our world a better place!\u00a0<\/p>\r\n<p><\/p>","protected":false},"excerpt":{"rendered":"<p>Table of Contents Introduction History of gene editing Pre-1960s with the discovery of double helix to producing DNA in a test tube 1960s \u2013 new developments concerning the structure and function of prokaryotic with the progress of viral genetic material 1970s \u2013 Witness the first Chimeric recombinant DNA cloning SV40 molecule into plasmid DNA 1980s [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":4875,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"off","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[3],"tags":[],"class_list":["post-4873","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-conditions"],"_links":{"self":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/posts\/4873","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/comments?post=4873"}],"version-history":[{"count":0,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/posts\/4873\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/media\/4875"}],"wp:attachment":[{"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/media?parent=4873"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/categories?post=4873"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.mybiosource.com\/learn\/wp-json\/wp\/v2\/tags?post=4873"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}