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LECT2 elisa kit :: Human Leukocyte cell-derived chemotaxin-2 ELISA Kit

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Catalog # MBS765240
Unit / Price
  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 Typical Testing Data/Standard Curve (for reference only)
Product Name

Leukocyte cell-derived chemotaxin-2 (LECT2), ELISA Kit

Popular Item
Full Product Name

Human Leukocyte cell-derived chemotaxin-2 ELISA Kit

Product Synonym Names
LECT2 (Leukocyte cell-derived chemotaxin-2)/Chondromodulin II/MIM1/chm2/chm-II/hLECT2/LECT-2/leukocyte cell-derived chemotaxin 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS765240 COA
OMIM
602882
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of LECT2. No significant cross-reactivity or interference between LECT2 and analogues was observed.
Samples
serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/ml
Sensitivity
<0.094ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LECT2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LECT2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LECT2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forLECT2purchase
MBS765240 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Leukocyte cell-derived chemotaxin-2 (LECT2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LECT2. The ELISA analytical biochemical technique of the MBS765240 kit is based on LECT2 antibody-LECT2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LECT2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LECT2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LECT2 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- LECT2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- LECT2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the LECT2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of LECT2 can be calculated.

Typical Testing Data/Standard Curve (for reference only) of LECT2 elisa kit
LECT2 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS765240. Click to request current manual
NCBI/Uniprot data below describe general gene information for LECT2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
16,390 Da
NCBI Official Full Name
leukocyte cell-derived chemotaxin-2
NCBI Official Synonym Full Names
leukocyte cell derived chemotaxin 2
NCBI Official Symbol
LECT2  [Similar Products]
NCBI Official Synonym Symbols
chm2; chm-II
  [Similar Products]
NCBI Protein Information
leukocyte cell-derived chemotaxin-2
UniProt Protein Name
Leukocyte cell-derived chemotaxin-2
UniProt Gene Name
LECT2  [Similar Products]
UniProt Synonym Gene Names
LECT-2; hLECT2  [Similar Products]
UniProt Entry Name
LECT2_HUMAN
NCBI Summary for LECT2
This gene encodes a secreted, 16 kDa protein that acts as a chemotactic factor to neutrophils and stimulates the growth of chondrocytes and osteoblasts. This protein has high sequence similarity to the chondromodulin repeat regions of the chicken myb-induced myeloid 1 protein. A polymorphism in this gene may be associated with rheumatoid arthritis. [provided by RefSeq, Jul 2008]
UniProt Comments for LECT2
LECT2: Has a neutrophil chemotactic activity. Also a positive regulator of chondrocyte proliferation. Belongs to the LECT2/MIM-1 family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: cytoplasm; extracellular space

Molecular Function: identical protein binding

Biological Process: chemotaxis; skeletal development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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