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LEP recombinant protein :: leptin antagonist Recombinant Protein

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Catalog # MBS400036
Unit / Price
  0.05 mg  /  $145 +1 FREE 8GB USB
  0.1 mg  /  $175 +1 FREE 8GB USB
  0.5 mg  /  $515 +1 FREE 8GB USB
  1 mg  /  $785 +1 FREE 8GB USB
  5 mg  /  $2,910 +3 FREE 8GB USB
  10 mg  /  $5,015 +6 FREE 8GB USB
LEP recombinant protein
Product Name

leptin antagonist, Recombinant Protein

Full Product Name

Ovine leptin antagonist (mutant L39A/D40A/F41A)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
The sequence of the first five N-terminal amino acids of ovine recombinan t leptin antagonist was determined and was found to be Ala-Val-Pr o-Ile-Arg
OMIM
164160
3D Structure
ModBase 3D Structure for P41159
Host
Escherichia Coli
Purity/Purification
The purity of ovine recombinant leptin antagonist is greater than 98.0% as determined by:
(a) Gel filtration analysis.
(b) Analysis by reducing and non-reducing SDS-PAGE gel.
Form/Format
White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized recombinant leptin antagonist in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
Protein quantitation of ovine recombinant leptin antagonist was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Dimers and Aggregates
The purified ovine recombinant leptin antagonist (16K) consists of > 95% monomers as determined by gel-filtration chromatography.
Endotoxin
The endotoxin content of ovine recombinant leptin antagonist is less than 0.1 ng/ug (IEU/ug) of ovine leptin antagonist.
Formulation
The recombinant leptin antagonist was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Biological Activity
ovine recombinant leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of ovine leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.
Preparation and Storage
Lyophilized ovine recombinant leptin antagonist although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution of ovine recombinant leptin antagonist at > 0.1 mg/ml and up to 2 mg and filter sterilization ovine recombinant leptin antagonist can be stored at +4 degree C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
Other Notes
Small volumes of LEP recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
LEP recombinant protein
Recombinant ovine leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, oLEP was mutated, resulting in L39A/D40A/F41A mutant was purified by proprietary chromatographic techniques. Preparation of recombinant leptin antagonists was recently published - Niv-Spector et al, Biochem. J 291;221-230 (2005).
Product Categories/Family for LEP recombinant protein
NCBI/Uniprot data below describe general gene information for LEP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
18,641 Da[Similar Products]
NCBI Official Full Name
leptin
NCBI Official Synonym Full Names
leptin
NCBI Official Symbol
NCBI Official Synonym Symbols
OB; OBS; LEPD
  [Similar Products]
NCBI Protein Information
leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); obese protein; obese, mouse, homolog of; obesity factor
UniProt Protein Name
Leptin
UniProt Synonym Protein Names
Obese protein; Obesity factor
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
OB; OBS  [Similar Products]
UniProt Entry Name
LEP_HUMAN
NCBI Summary for LEP
This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]
UniProt Comments for LEP
leptin: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the leptin family.

Protein type: Hormone; Secreted, signal peptide; Cell development/differentiation; Secreted

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: peptide hormone receptor binding; growth factor activity; hormone activity

Biological Process: circadian rhythm; response to dietary excess; positive regulation of myeloid cell differentiation; regulation of fat cell differentiation; regulation of steroid biosynthetic process; female pregnancy; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; negative regulation of appetite; positive regulation of luteinizing hormone secretion; response to insulin stimulus; positive regulation of tyrosine phosphorylation of Stat3 protein; response to vitamin E; regulation of cholesterol absorption; positive regulation of MAPKKK cascade; regulation of blood pressure; positive regulation of cell proliferation; positive regulation of ion transport; central nervous system neuron development; placenta development; positive regulation of cytokine production; cholesterol metabolic process; positive regulation of developmental growth; eating behavior; bile acid metabolic process; glucose metabolic process; adult feeding behavior; ovulation from ovarian follicle; leptin-mediated signaling pathway; negative regulation of vasoconstriction; tyrosine phosphorylation of STAT protein; fatty acid beta-oxidation; glycerol biosynthetic process; insulin secretion; response to hypoxia; energy reserve metabolic process; hormone metabolic process; regulation of gluconeogenesis; positive regulation of insulin receptor signaling pathway; positive regulation of follicle-stimulating hormone secretion; leukocyte tethering or rolling; regulation of insulin secretion; negative regulation of apoptosis

Disease: Leptin Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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