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LEPR elisa kit :: Human leptin receptor ELISA Kit

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Catalog # MBS703701
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $435 +1 FREE 8GB USB
  96-Strip-Wells  /  $625 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

leptin receptor (LEPR), ELISA Kit

Popular Item
Also Known As

Human Leptin receptor, LR/Ob-R ELISA Kit

Product Synonym Names
Human Leptin receptor; LR/Ob-R ELISA KIT; CD295; OBR; OB receptor; OTTHUMP00000010694; leptin receptor
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS703701 Testing Data
Sequence Length
896
OMIM
gene 614963
3D Structure
ModBase 3D Structure for P48357
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human LEPR. No significant cross-reactivity or interference between Human LEPR and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Detection Range
3.12 pg/ml-200 pg/ml
Sensitivity
0.78 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LEPR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forLEPRpurchase
MBS703701 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the leptin receptor (LEPR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LEPR. The ELISA analytical biochemical technique of the MBS703701 kit is based on LEPR antibody-LEPR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LEPR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LEPR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LEPR elisa kit
For samples: This immunoassay kit allows for the in vitro quantitative determination of human LR/Ob-R concentrations in serum, plasma, cell Culture supernates, tissue homogenates.

PRINCIPLE OF THE ASSAY The microtiter plate provided in this kit has been pre-coated with an antibody specific to LR/Ob-R. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for LR/Ob-R and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain LR/Ob-R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LR/Ob-R in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of LEPR elisa kit
LEPR elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS703701. Click to request current manual
NCBI/Uniprot data below describe general gene information for LEPR. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
132,494 Da
NCBI Official Full Name
leptin receptor isoform 3
NCBI Official Synonym Full Names
leptin receptor
NCBI Official Symbol
NCBI Official Synonym Symbols
OBR; OB-R; CD295; LEP-R; LEPRD
  [Similar Products]
NCBI Protein Information
leptin receptor; huB219; OB receptor
UniProt Protein Name
Leptin receptor
UniProt Synonym Protein Names
HuB219; OB receptor; OB-R; CD_antigen: CD295
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
DB; OBR; LEP-R; OB-R  [Similar Products]
UniProt Entry Name
LEPR_HUMAN
NCBI Summary for LEPR
The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]
UniProt Comments for LEPR
Function: Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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